摘要
【目的】了解普通油茶(Camellia oleifera)果实发育过程中种仁代谢物的变化。【方法】以普通油茶不同发育阶段(F1~F5)的种仁为材料,结合液相色谱与质谱联用(LC-MS/MS)代谢组学技术,分析普通油茶种仁发育过程中代谢物的变化规律。【结果】共鉴定到15类1006种代谢物,主要包括脂类和类脂分子,苯丙素类和聚酮类,有机酸及其衍生物,芳烃类,有机氧化合物,杂环化合物,核苷、核苷酸和类似物;被注释的主要代谢通路包括代谢途径、次生代谢产物的生物合成、植物次生代谢产物的生物合成、氨基酸的生物合成、2-草酸代谢。不同发育时期普通油茶种仁的代谢物有显著差异,F1和F2的种仁代谢物最少,仅有3-甲基-1-(2,4,6-三羟基苯基)-1-丁酮、L-谷胱甘肽、蔗糖,说明种仁前期缓慢生长;F3的种仁代谢物最多,尤其是苯丙素类和聚酮类、芳烃类、脂类和类脂分子、有机酸及其衍生物、有机杂环化合物的相对含量变化明显,其中黑色素、酪氨酸、苯丙氨酸、L-谷胱甘肽、1-甲基鸟嘌呤、3,4-二氢-2H-1-苯并吡喃-2-酮、(S)-匹诺森布林、山柰酚-3-O-芸香糖苷的相对含量较高,说明其在果实快速膨大期种仁成型发挥重要作用;F4和F5的代谢物逐渐减少,种仁进入油脂积累期。【结论】本研究运用代谢组学方法了解普通油茶果实发育过程中种仁代谢物的变化规律,可为普通油茶果优质丰产栽培和适时采收提供理论依据。
[Purpose]To understand the changes of kernel metabolites during the development of Camellia oleifera fruits.[Methods]Using different developmental stages of C.oleifera kernels as materials,combined with liquid chromatography mass spectrometry(LC-MS/MS) metabolomics techniques,the metabolic changes of C.oleifera kernels during development were analyzed.[Results]A total of 1 006 differential metabolites were identified,belonging 15 categories,mainly including lipids and lipid-like molecules,phenylpropane and polyketones,organic acids and their derivatives,benzenoids,organic oxygen compounds,organoheterocyclic compounds,nucleosides,nucleotides and analogs.The annotated main metabolic pathways included metabolic pathways,biosynthesis of secondary metabolites,biosynthesis of plant secondary metabolites,biosynthesis of amino acids,and metabolism of 2-oxalic acid.There were significant differences in metabolites in the kernels of C.oleifera at different developmental stages.F1 and F2 periods had the least metabolites,only including 3-methyl-1-(2,4,6-trihydroxyphenyl)-1-butanone,L-glutathione,and sucrose,indicating slow growth of the kernels in the early stages.In F3 period,there were the largest number of kernel metabolites,especially the relative contents of phenylpropane and polyketones,benzenoids,lipids and lipid molecules,organic acids and their derivatives,and organic heterocyclic compounds changed obviously;the relative contents of melanin,tyrosine,phenylalanine,L-glutathione,1-methylnenenebc guanine,3,4-dihydro-2H-1-benzopyran-2-one,(S)-pinocenbrin,and kaempferol-3-O-rutoside were relatively high,indicating that it played an important role in the formation of kernels during the rapid expansion period of fruits.The metabolites in F4 and F5 periods gradually decreased,and the kernel entered the oil accumulation period.[Conclusion]This study uses metabolomics methods to understand the changes in kernel metabolites during the development process of C.oleifera fruits,which can provide a theoretical basis for high-quality and high-yield cultivation and timely harvesting of C.oleifera fruits.
作者
龙雪燕
闫道良
李万春
郑炳松
胡玉玲
LONG Xueyan;YAN Daoliang;LI Wanchun;ZHENG Bingsong;HU Yuling(State Key Laboratory of Subtropical Silviculture,Zhejiang Provincial Key Laboratory of Forest Aromatic Plants-based Healthcare Function,School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China;College of Agriculture and Forestry Science and Technology,Hunan Applied Technology University,Changde 415000,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第3期116-126,共11页
Journal of Yunnan Agricultural University:Natural Science
基金
湖南应用技术学院博士科研启动项目(2023HYBS01)
常德市经济林生态过程调控与高值化利用工程技术研究中心项目
湖南省油茶产业科研示范项目
湖南省教育厅重点项目(22A0714,22C1211)
湖南省“十四五”应用特色学科(林学)(湘教通[2022]351)。
关键词
普通油茶
果实发育进程
种仁
代谢组
Camellia oleifera
development process of fruit
seed kernel
metabolome
作者简介
龙雪燕(1998-),女,贵州铜仁人,在读硕士研究生,主要从事经济林木发育与栽培生理研究。E-mail:2363078950@qq.com;通信作者:郑炳松(1972-),男,浙江龙游人,博士,教授,主要从事林木培育与利用研究。E-mail:bszheng@zafu.edu.cn;通信作者:胡玉玲(1977-),男,浙江温州人,博士,教授,主要从事油茶育种与栽培研究。E-mail:huyulin@126.com。
