摘要
以抗寒性不同的白菜型冬油菜品种陇油7号与陇油9号为亲本构建的F2群体为材料,对白菜型冬油菜抗寒性相关的生理指标进行了QTL定位分析。在白菜型油菜的10条染色体上定位了SOD活性、POD活性、CAT活性、MDA含量、游离脯氨酸含量和可溶性蛋白含量共6个性状的24个QTL位点,可解释表型变异为11.1783%~81.1753%;10个显性效应表现为显性正效应,LOD值在3.2787~163.7958之间。染色体2A的Br ID90127-Br ID10421区、Br ID10421-Br ID10709区及Br ID10709-Br ID101165区,5A的BRMS034(R5)-Ra3-H10区、6A的Ra1-F06-Ra2-D04区、8A的Br ID10839-Ra2-E12区和10A的Br ID90115-Ra2-E07区是多个性状QTL共享的标记区间。研究结果对冬油菜抗寒性基因精确定位具有一定价值。
The superior cold resistance B. rapa variety ＂Longyou 7＂and general cold variety ＂Longyou 9＂were used as parents and a F2 mapping population was constructed. The results showed that 39 primers were selected for further analysis of F2 population. Based on molecular marker genetic map and QTL software,a total of 24 QTLs were detected for SOD activity,POD activity,CAT activity,MDA content,free proline content and soluble protein content from 10 chromosomes. Those QTLs were able to explain 11. 1783% - 81. 1753% of phenotypic variation. The dominant effects of10 QTLs were positive effects,LOD values were between 3. 2787 - 163. 7958. The data showed that the regions Br ID90127-Br ID10421,Br ID10421-Br ID10709 and Br ID10709-Br ID101165 on 2A,BRMS034（ R5）-Ra3-H10 on5 A,Ra1-F06-Ra2-D04 on 6A,Br ID10839-Ra2-E12 on 8A,Br ID90115-Ra2-E07 on 10 A were common regions shared by multi-traits. The result of present study is useful for the accurate position of winter rapa.
作者
武军艳
孔德晶
方彦
孙万仓
刘海卿
刘自刚
李学才
WU Jun-yan KONG De-jing FANG Yan SUN Wan-cang LIU Hai-qing LIU Zi-gang LI Xue-cai(College of Agronomy, C.ansu Agricultural University, C.ansu Key Lab of Crop Improvement and Germplasm Enhancement, C.ansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou , C.ansu 730070, Chin)
出处
《干旱地区农业研究》
CSCD
北大核心
2017年第1期205-210,共6页
Agricultural Research in the Arid Areas
基金
甘肃省青年科技基金计划(145RJYA253)
农业部产业技术体系建设资金项目(CARS-13)
甘肃省自然科学基金项目(145RJZG050)
关键词
白菜型冬油菜
抗寒性
QTL
北方旱寒区
winter rapeseed（Brassica rapa L.）
cold resistance
QTL
northern arid-cold area
作者简介
武军艳（1981-），女，甘肃平川人，讲师，在读博士，主要从事油菜的育种工作。E-mail：wujuny@gsau．edu．cn。
通信作者：孙万仓（1957-），男，教授，博士，主要从事油菜的育种工作。E-mail：18293121851@163．com．cn。
