摘要
目的观察急性淋巴细胞白血病患儿细胞因子水平与疾病状态的关系。方法选取2018年1月至2019年12月贵阳市妇幼保健院收治的212例急性淋巴细胞白血病患儿纳入急性淋巴细胞白血病组,根据患儿不同疾病状态将其分为初诊组(128例)、缓解组(84例),并将同期在该院常规体检的200例健康儿童纳入对照组,同期收治的50例急性髓细胞白血病患儿纳入急性髓细胞白血病组,对比各组血清白细胞介素(IL)-2、IL-10、肿瘤坏死因子(TNF)-α、白细胞计数(WBC)水平,并分析初诊急性淋巴细胞白血病患儿血清IL-2、IL-10、TNF-α、WBC水平与临床特征的相关性。随访6个月,根据急性淋巴细胞白血病患儿有无复发分为复发组(72例)与未复发组(140例),比较各组细胞因子水平。结果急性淋巴细胞白血病组TNF-α、IL-10、WBC水平高于急性髓细胞白血病组与对照组,IL-2水平低于急性髓细胞白血病组与对照组,差异有统计学意义(P<0.05)。初诊组IL-2水平明显低于缓解组,TNF-α、IL-10、WBC水平高于缓解组,差异有统计学意义(P<0.05)。初诊急性淋巴细胞白血病患儿血清IL-2、IL-10、TNF-α、WBC水平与患儿年龄、性别及肝大等无关(P>0.05),与患儿疾病危险程度分型有关(P<0.05)。与未复发组相比,复发组血清IL-2水平降低,IL-10、WBC、TNF-α水平升高,差异有统计学意义(P<0.05)。结论急性淋巴细胞白血病患儿血清IL-2水平降低,TNF-α、IL-10、WBC水平升高,以上指标水平变化能够反映出患儿免疫功能好坏,可作为疾病状态及预后的评估指标,判断患儿疾病复发风险。
Objective To observe the relationship between the cytokines levels and disease status in children with acute lymphoblastic leukemia(ALL).Methods A total of 212 children patients with ALL admitted to Guiyang Municipal Maternal and Child Health Care Hospital from January 2018 to December 2019 were selected in the ALL group.According to their different disease statuses,the cases were divided into the newly diagnosed group(128 cases)and remission group(84 cases).Contemporaneous 200 healthy children undergoing the routine physical examination in this hospital were included in the control group and 50 children patients with acute myeloid leukemia(AML)were included in the AML group.The levels of serum interleukin(IL-2),IL-10,tumor necrosis factor(TNF)-αand white blood cell count(WBC)were compared among the groups.The correlation between serum IL-2,IL-10,TNF-αand WBC levels with the clinical characteristics in newly diagnosed children patients with ALL was analyzed.After 6-month follow-up,the children patients with ALL were divided into the recurrent group(72 cases)and non-recurrent group(140 cases)according to whether there was recurrence or not,and the cytokine levels were compared between the groups.Results The levels of TNF-α,IL-10 and WBC in the ALL group were higher than those in the AML group and control group,while the IL-2 level was lower than that in the AML group and control group,and the differences were statistically significant(P<0.05).The IL-2 level in the newly diagnosed group was significantly lower than that in the remission group,while the levels of TNF-α,IL-10 and WBC were higher than those in the remission group,and the differences were statistically significant(P<0.05).The levels of serum IL-2,IL-10,TNF-αand WBC in the children patients with newly diagnosed ALL had no correlation with the age,sex and hepatomegaly,etc(P>0.05),but had close correlation with the risk degree of disease(P<0.05).Compared with the non-recurrence group,the serum IL-2 level in the recurrence group was decreased,while the levels of IL-10,WBC and TNF-αwere increased,and the differences were statistically significant(P<0.05).Conclusion The serum IL-2 level in the children patients with ALL is decreased,while the serum TNF-α,IL-10 and WBC levels are increased.The levels change of above indexes can reflect the good or bad status of immune function in children patients,which can serve as the evaluation indexes of disease status and prognosis to judge the risk of disease recurrence.
作者
宋晓梅
李化
舒莉萍
SONG Xiaomei;LI Hua;SHU Liping(State and Local Joint Engineering Laboratory of Cell Engineering and Biomedical Medical Technology/Guizhou Provincial Key Laboratory of Regenerative Medicine/Teaching and Researching Section of Immunology,School of Basic Medicine,Guizhou Medical University,Guiyang,Guizhou 550004,China;Department of Clinical Laboratory,Guiyang Municipal Maternal and Child Health Care Hospital,Guiyang,Guizhou 550004,China;Key Laboratory of Adult Stem Cell Transformation,Chinese Academy of Medical Sciences,Guiyang,Guizhou 550004,China)
出处
《国际检验医学杂志》
CAS
2021年第15期1816-1820,共5页
International Journal of Laboratory Medicine
基金
贵州省科学技术厅科技项目(黔科合平台人才[2017]5611号,黔科合基础[2019]1436号,黔科中引地[2019]4008号)。
作者简介
宋晓梅,女,主管技师,主要从事干细胞与血液免疫研究;通信作者:舒莉萍,E-mail:gyslp-456@163.com。
