After current zero,which is the moment when the vacuum circuit breaker interrupts a vacuum arc,sheath development is the first process in the dielectric recovery process.An axial magnetic field（AMF） is widely used i...After current zero,which is the moment when the vacuum circuit breaker interrupts a vacuum arc,sheath development is the first process in the dielectric recovery process.An axial magnetic field（AMF） is widely used in the vacuum circuit breaker when the high-current vacuum arc is interrupted.Therefore,it is very important to study the influence of different AMF amplitudes on the sheath development.The objective of this paper is to study the influence of different AMF amplitudes on the sheath development from a micro perspective.Thus,the particle in cell-Monte Carlo collisions（PIC-MCC） method was adopted to develop the sheath development model.We compared the simulation results with the experimental results and then validated the simulation.We also obtained the speed of the sheath development and the energy density of the ions under different AMF amplitudes.The results showed mat the larger the AMF amplitudes are,the faster the sheath develops and the lower the ion energy density is,meaning the breakdown is correspondingly more difficult.展开更多
A multi-body model of engine system with flexible crankshaft was presented in this paper to analyze the dynamic behavior of an internal combustion engine. The flexible crankshaft structural dynamics was coupled with t...A multi-body model of engine system with flexible crankshaft was presented in this paper to analyze the dynamic behavior of an internal combustion engine. The flexible crankshaft structural dynamics was coupled with the main beating hydrodynamic lubrication in this model by a system approach. An application of an 14 engine was given to show this sophisticated simulation model and to predict the loads and the orbit plots in the journal beatings by the dynamic response of the multi-body engine system with flexible crankshaft. The numerical results show the capabilities and significance of the flexible crankshaft in this system. The objective of the research is to provide the scientific guidance for design and maintenance of the internal combustion engine.展开更多
In this paper,using the existence and comparison result for the quasi-monotone increasing system developed by C V Pao,the upper and lower solutions principle and an iterative method,we investigate the existence of the...In this paper,using the existence and comparison result for the quasi-monotone increasing system developed by C V Pao,the upper and lower solutions principle and an iterative method,we investigate the existence of the positive solutions of the Volterra-Lotka cooperating model.展开更多
In this paper, a kind of multi-level formal concept is introduced. Based on the proposed multi-level formal concept,we present a pair of rough fuzzy set approximations within fuzzy formal contexts.By the proposed roug...In this paper, a kind of multi-level formal concept is introduced. Based on the proposed multi-level formal concept,we present a pair of rough fuzzy set approximations within fuzzy formal contexts.By the proposed rough fuzzy set approximations,we can approximate a fuzzy set according to different precision level.We discuss the properties of the proposed approximation operators in detail.展开更多
Objective To explore the feasibility and efficiency of a novel magnetic compression anastomats(MCAs) in intestinal anastomosis.Methods A total of 36 male mongrel canines underwent intestinal anastomosis using traditio...Objective To explore the feasibility and efficiency of a novel magnetic compression anastomats(MCAs) in intestinal anastomosis.Methods A total of 36 male mongrel canines underwent intestinal anastomosis using traditional hand-sewn(n=18) or a novel MCAs(n=18).We compared the anastomosis time,postoperative complications,bursting strength of anastomoses,gross appearance,and pathology between two groups at each time-point of follow-up.Results The mean anastomosis time with MCAs was significantly less than that with hand-sewn(8.50±1.95 vs.31.1±4.32 minutes,P<0.001).The blood stools and intussusceptions occurred in both groups during follow-up period.Only 1 mongrel canine receiving intestinal anastomosis by MCAs experienced anastomotic leakage.The average bursting pressure of anastomoses obtained from mongrel canines undergoing intestinal anastomosis by MCAs was significantly higher than that by traditional hand-sewn at 1 week's follow-up time(P<0.05).Gross appearance of the anastomoses constructed by MCAs was relatively smoother and flatter.Pathological evalution of anastomoses revealed that general inflammation was greater in hand-sewn anastomoses than magnetic anastomosis.Conclusion The magnetic compression anastomat is a safe and effective device of sutureless intestinal anastomosis in canine models.展开更多
The Molten Salt Reactor (MSR) can meet the demand of transmutation and breeding. In this study, theoretical calculation of steady thermal hydraulic characteristics of a graphite-moderated channel type MSR is conducted...The Molten Salt Reactor (MSR) can meet the demand of transmutation and breeding. In this study, theoretical calculation of steady thermal hydraulic characteristics of a graphite-moderated channel type MSR is conducted. The DRAGON code is adopted to calculate the axial and radial power factor firstly. The flow and heat transfer model in the fuel salt and graphite are developed on basis of the fundamental mass, momentum and energy equations. The results show the detailed flow distribution in the core, and the temperature profiles of the fuel salt, inner and outer wall in the nine typical elements along the axial flow direction are also obtained.展开更多
Chattering phenomenon and singularity are still the main problems that hinder the practical application of sliding mode control. In this paper, a fixed time integral sliding mode controller is designed based on fixed ...Chattering phenomenon and singularity are still the main problems that hinder the practical application of sliding mode control. In this paper, a fixed time integral sliding mode controller is designed based on fixed time stability theory, which ensures precise convergence of the state variables of controlled system, and overcomes the drawback of convergence time growing unboundedly as the initial value increases in finite time controller. It makes the controlled system converge to the control objective within a fixed time bounded by a constant as the initial value grows, and convergence time can be changed by adjusting parameters of controllers properly. Compared with other fixed time controllers, the fixed time integral sliding mode controller proposed in this paper achieves chattering-free control, and integral expression is used to avoid singularity generated by derivation. Finally, the controller is used to stabilize four-order chaotic power system. The results demonstrate that the controller realizes the non-singular chattering-free control of chaotic oscillation in the power system and guarantees the fixed time convergence of state variables, which shows its higher superiority than other finite time controllers.展开更多
Objective To construct an expression vector of small interfering RNA （siRNA） against survivin and observe its effects on survivin expression and proliferation of human pancreatic cancer cell line PC-2 and breast can...Objective To construct an expression vector of small interfering RNA （siRNA） against survivin and observe its effects on survivin expression and proliferation of human pancreatic cancer cell line PC-2 and breast cancer cell line MCF-7. Methods Constructed an expression vector of siRNA against survivin and transfected it into PC-2 and MCF-7 cells using lipofectamine^TM 2000. The expression of survivin was detected by semi-quanfifive RT-PCR and immunohistochemistry, and its effects on proliferation of PC-2 and MCF-7 cells were detected by MTT assay. Results The introduction of sequence-specific siRNA could efficiently suppress survivin expression at both mRNA and protein levels in the two cancer cell lines. In PC-2 cells, the expression inhibition rates were 81.25% at mRNA level and 74.24% at protein level In MCF-7 cells, the expression inhibition rates were 64.91% at mRNA level and 79. 72% at protein level The proliferation of PC-2 and MCF-7 cells was also suppressed, and24 and 48 hours after the cells were reseeded, the proliferation inhibition rates of PC-2 cells were 28. 00% and 33. 38%, and that of MCF-7 cells were 31.58% and 33.02%, respectively. Conclusions The expression vector of siRNA against survivin can block survivin expression in PC-2 and MCF-7 cells efficiently and specifically. Down regulation of survivin expression can suppress proliferation of PC-2 and MCF-7 cells. Survivin RNAi may have potential value in gene therapy of human cancers.展开更多
In this paper a mathematical model is built for a buried hot crude oil pipeline during shutdown, and an unstructured grid and polar coordinate grid are respectively applied to generating grids for the soil region and ...In this paper a mathematical model is built for a buried hot crude oil pipeline during shutdown, and an unstructured grid and polar coordinate grid are respectively applied to generating grids for the soil region and the three layers in the pipe （wax layer, pipe wall, and corrosion-inhibiting coating）. The governing equations are discretized using the finite volume method. The variations in temperatures of static oil and soil were investigated during pipeline shutdown in both summer and winter, in which some important parameters of the soil and crude oils of a Northeast pipeline are employed.展开更多
Background The objective of this study was to assess the clinical safety and efficacy of vena cava filter （VCF） placement, with particular emphasis on the incidence and risk factors of inferior vena cava thrombosis ...Background The objective of this study was to assess the clinical safety and efficacy of vena cava filter （VCF） placement, with particular emphasis on the incidence and risk factors of inferior vena cava thrombosis （VCT） after VCF placement. Methods Clinical data of patients with venous thromboembolism （VTE）, with or without placement of VCF, were analyzed in a retrospective single-center audit of medical records from January 2005 to June 2009. The collected data included demographics, procedural details, filter type, indications, and complications. Results A total of 168 cases of VTE （82 with VCF; 86 without VCF） were examined. Over a median follow-up of 24.2 months, VCT occurred in 18 of 82 patients with VCFs （11 males, 7 females, mean age 55.4 years）. In 86 patients without VCFs, VCT occurred in only 6 individuals （4 males, 2 females） during the study period. VCT was observed more frequently in patients fitted with VCFs than in those without VCFs （22% vs. 7.0%）. Conclusions The incidence of VCT in patients with VTE after VCF implantation was 22% approximately. Anticoagulation therapy should be continued for all patients with VCF placement, unless there is a specific contraindication. Almost all instances of VCT in patients with VCF implants in our study occurred after stopping anticoagulation treatment. The use of VCFs is increasing, and more trials are needed to confirm their benefit and accurately assess their safety.展开更多
Drying （conditioning） is an important procedure to prevent hydrate formation during gas pipeline gas-up and to protect pipelines against corrosion. The air-drying method is preferred in offshore gas pipelines pre-co...Drying （conditioning） is an important procedure to prevent hydrate formation during gas pipeline gas-up and to protect pipelines against corrosion. The air-drying method is preferred in offshore gas pipelines pre-commissioning. The air-drying process of gas pipelines commonly includes two steps, air purging and soak test. The mass conservation and the phase equilibrium theory are applied to setting up the mathematical models of air purging, which can be used to simulate dry airflow rate and drying time. Fick diffusion law is applied to setting up the mathematical model of soak test, which can predict the water vapor concentration distribution. The results calculated from the purging model and the soak test model are in good agreement with the experimental data in the DF 1-1 offshore production pipeline conditioning. The models are verified to be available for the air-drying project design of offshore gas pipelines. Some proposals for airdrying engineering and operational procedures are put forward by analyzing the air-drying process of DFI-1 gasexporting pipelines.展开更多
Chokes are one of the most important components of downhole flow-control equipment. The particle erosion mathematical model, which considers particle-particle interaction, was established and used to simulate solid pa...Chokes are one of the most important components of downhole flow-control equipment. The particle erosion mathematical model, which considers particle-particle interaction, was established and used to simulate solid particle movement as well as particle erosion characteristics of the solid-liquid two-phase flow in a choke. The corresponding erosion reduction approach by setting ribs on the inner wall of the choke was advanced. This mathematical model includes three parts： the flow field simulation of the continuous carrier fluid by an Eulerian approach, the particle interaction simulation using the discrete particle hard sphere model by a Lagrangian approach and calculation of erosion rate using semiempirical correlations. The results show that particles accumulated in a narrow region from inlet to outlet of the choke and the dominating factor affecting particle motion is the fluid drag force. As a result, the optimization of rib geometrical parameters indicates that good anti-erosion performance can be achieved by four ribs, each of them with a height （H） of 3 mm and a width （B） of 5 mm equaling the interval between ribs （L）.展开更多
Needs in scintimammography applications,especially for small animal cardiac imaging,lead to develop a small field of view,high spatial resolution gamma camera with a pinhole collimator.However the ideal pinhole collim...Needs in scintimammography applications,especially for small animal cardiac imaging,lead to develop a small field of view,high spatial resolution gamma camera with a pinhole collimator.However the ideal pinhole collimator must keep a compromise between spatial resolution and sensitivity.In order to design a pinhole collimator with an optimized sensitivity and spatial resolution,the spatial resolution and the geometric sensitivity response as a function of the source to collimator distance has been obtained by means of Monte-Carlo simulation for a small field of view gamma camera with a pinhole collimator of various-hole diameters.The results show that the camera with pinhole of 1 mm,1.5 mm and 2 mm diameter has respectively spatial resolution of 1.5 mm,2.25 mm and 3 mm and geometric sensitivity of 0.016%,0.022%and 0.036%,while the source to collimator distance is 3 cm.We chose the pinhole collimator with hole diameter size of 1.2 mm for our the gamma camera designed based on the trade-off between sensitivity and resolution.展开更多
In this paper,work was conducted to reveal electrical tree behaviors（initiation and propagation）of silicone rubber（SIR） under an impulse voltage with high temperature.Impulse frequencies ranging from 10 Hz to 1 k ...In this paper,work was conducted to reveal electrical tree behaviors（initiation and propagation）of silicone rubber（SIR） under an impulse voltage with high temperature.Impulse frequencies ranging from 10 Hz to 1 k Hz were applied and the temperature was controlled between 30 °C and 90 °C.Experimental results show that tree initiation voltage decreases with increasing pulse frequency,and the descending amplitude is different in different frequency bands.As the pulse frequency increases,more frequent partial discharges occur in the channel,increasing the tree growth rate and the final shape intensity.As for temperature,the initiation voltage decreases and the tree shape becomes denser as the temperature gets higher.Based on differential scanning calorimetry results,we believe that partial segment relaxation of SIR at high temperature leads to a decrease in the initiation voltage.However,the tree growth rate decreases with increasing temperature.Carbonization deposition in the channel under high temperature was observed under microscope and proven by Raman analysis.Different tree growth models considering tree channel characteristics are proposed.It is believed that increasing the conductivity in the tree channel restrains the partial discharge,holding back the tree growth at high temperature.展开更多
A thermogravimetric analysis (TG) was conducted to study the thermal decomposition behavior and kinetics of composites from coal and high density polyethylene (HDPE), linear low density polyethylene (LLDPE) or low den...A thermogravimetric analysis (TG) was conducted to study the thermal decomposition behavior and kinetics of composites from coal and high density polyethylene (HDPE), linear low density polyethylene (LLDPE) or low density polyethylene (LDPE). The results show that coal facilitates melting of the polyethylene before temperatures reach 700 K in nitrogen due to the exothermic effect of coal. Above 700 K, adding coal into the polyethylene will result in smaller maximum rates of mass loss and higher initial mass loss temperatures of the composites. Hence, some chemical interactions, occurring between liquid compounds released in the pyrolysis of the coal and polymer, depend on several factors, such as coal rank and the molecular structure of polymers. Synergetic effects in coal and polymers were also found. Both chemical interactions and synergetic effects control the entire thermal decomposition behavior of compos- ites. The larger the amount of coal in the composites, the greater the decomposition temperature spans and the higher the maximum decomposition temperature, the smaller the devolatilization rates. The effect of coal on the thermal stability of composites lies in the hydrogen acceptor effect of the coals. Thermal decomposition of the coals, the polymers and related composites can be modelled via first order parallel reactions between 563 K and 763 K.展开更多
The method of characteristic(MOC) was adopted to analyze the check valve-induced water hammer behaviors for a Parallel Pumps Feedwater System(PPFS) during the alternate startup process.The motion of check valve disc w...The method of characteristic(MOC) was adopted to analyze the check valve-induced water hammer behaviors for a Parallel Pumps Feedwater System(PPFS) during the alternate startup process.The motion of check valve disc was simulated using inertial valve model.Transient parameters including the pressure oscillation,local flow velocity and slamming of the check valve disc etc.have been obtained.The results showed that severe slamming between the valve disc and valve seat occurred during the alternate startup of parallel pumps.The induced maximum pressure vibration amplitude is up to 5.0 MPa.The scheme of appending a damping torque to slow down the check valve closing speed was also performed to mitigate of water hammer.It has been numerically approved to be an effective approach.展开更多
Although the two-grid finite element decoupled scheme for mixed Navier-Stokes/ Darcy model in literatures has given the numerical results of optimal convergence order, the theoretical analysis only obtain the optimal ...Although the two-grid finite element decoupled scheme for mixed Navier-Stokes/ Darcy model in literatures has given the numerical results of optimal convergence order, the theoretical analysis only obtain the optimal error order for the porous media flow and a non-optimal error order for the fluid flow. In this article, we give a more rigorous of the error analysis for the fluid flow and obtain the optimal error estimates of the velocity and the pressure.展开更多
Objective To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants. Methods Forty mice, thirty-two rats, and twenty-four rabbits were randomly and equally divided into control grou...Objective To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants. Methods Forty mice, thirty-two rats, and twenty-four rabbits were randomly and equally divided into control group (saline) and three ellagic acid (EA)-treated groups (low, middle, and high doses). In the mice, bleeding time (BT) was estimated with tail transaction, and clotting time (CT) with template method. Prothrombin time (PT) and the activated partial thromboplastin time (APTT) in rats and rabbits were measured by means of Quick's one-stage assay and modified APTT assay respectively. In addition, thrombin activity was estimated in rats with PT assay using a hemagglutination analyzer. The circulating platelet aggregates were de- tected in rabbits through platelet counting and presented as the circulating platelet aggregate ratio (CPAR). Results EA shortened BT and CT in mice, PT and APTT in rats, and increased thrombin activity and CPAR, all in a dose-dependent manner. EA also brought reduction of PT and APTT in rabbits in dose- and time-dependent manners. Conclusion EA could induce hypercoagulable state through activating coagulation system and platelets in mice, rats, and rabbits.展开更多
Objective To investigate the levels of cardiovascular disease risk factors and their relations to clinical phenotype associated with coronary artery disease(CAD).Methods The subjects were recruited from five independe...Objective To investigate the levels of cardiovascular disease risk factors and their relations to clinical phenotype associated with coronary artery disease(CAD).Methods The subjects were recruited from five independent cardiovascular centers.Coronary angiography was employed to define the CAD with stenosis in each major vessel ≥70% and control with stenosis <10% in every lesion.The classic risk factors including family history,body mass index,smoking habits,hypertension,diabetes mellitus,and serum lipid levels were surveyed according to established criteria.Associations between risk levels and clinical phenotypes were assessed by case control and correlation analysis.Results A total of 762 individuals were collected,including 481 men and 281 women,aged from 17 to 81(mean 60±10) years.The patients with CAD accounted for 55.5% of all participants,and controls 44.5%,respectively.Compared with the pattern in published data,our study showed that mean serum high density lipoprotein cholesterol(HDL-C) level was significantly lower(P<0.001) and triglycerides was significantly higher(P<0.001),while total cholesterol(TC) and low density lipoprotein cholesterol levels were comparative(both P>0.05).The prevalence of low HDL-C(<40 g/L) and hypertriglyceridemia(>150 g/L) were 27.2% and 41.4%,respectively.Mean serum levels of HDL-C and apolipoprotein A1 were significantly higher in female subjects than in male(P<0.001).Lower HDL-C functioned as an independent risk factor for CAD only in men(RR=2.8,95%CI:1.5-4.2,P<0.001),yet increased non-HDL cholesterol combined with diabetes mellitus and obesity seemed to play a key role in the development ofCAD in women.Similarity in risk association with CAD was found for hypertension and TC/HDL ratio in male and female subjects,while family history had no relationship with the presence of CAD.Conclusion It is remarkable that emphasis of intervention in future should be given on the prevalent low serum HDL-C and its strong risk correlation with the presence of CAD in male subjects of Chinese Han population.展开更多
基金supported by the National Key Basic Research Program of China(973 Program) 2015CB251002National Natural Science Foundation of China under Grant 51521065, 51577145,51377128,51323012,51607135+3 种基金Program of State Grid Electrical Power Research Institute GY71-14-004the Science and Technology Project Funds of the Grid State Corporation(Medium voltage DC distribution protection) (SGSNKYOOKJJS1501564)the Science and Technology Project Funds of Hubei Electric Power Company(SGRIZLKJ (2016)325)State Key Laboratory of Electrical Insulation and Power Equipment(EIPE17305)
文摘After current zero,which is the moment when the vacuum circuit breaker interrupts a vacuum arc,sheath development is the first process in the dielectric recovery process.An axial magnetic field（AMF） is widely used in the vacuum circuit breaker when the high-current vacuum arc is interrupted.Therefore,it is very important to study the influence of different AMF amplitudes on the sheath development.The objective of this paper is to study the influence of different AMF amplitudes on the sheath development from a micro perspective.Thus,the particle in cell-Monte Carlo collisions（PIC-MCC） method was adopted to develop the sheath development model.We compared the simulation results with the experimental results and then validated the simulation.We also obtained the speed of the sheath development and the energy density of the ions under different AMF amplitudes.The results showed mat the larger the AMF amplitudes are,the faster the sheath develops and the lower the ion energy density is,meaning the breakdown is correspondingly more difficult.
基金Project 50375115 supported by the National Natural Science Foundation of China
文摘A multi-body model of engine system with flexible crankshaft was presented in this paper to analyze the dynamic behavior of an internal combustion engine. The flexible crankshaft structural dynamics was coupled with the main beating hydrodynamic lubrication in this model by a system approach. An application of an 14 engine was given to show this sophisticated simulation model and to predict the loads and the orbit plots in the journal beatings by the dynamic response of the multi-body engine system with flexible crankshaft. The numerical results show the capabilities and significance of the flexible crankshaft in this system. The objective of the research is to provide the scientific guidance for design and maintenance of the internal combustion engine.
文摘In this paper,using the existence and comparison result for the quasi-monotone increasing system developed by C V Pao,the upper and lower solutions principle and an iterative method,we investigate the existence of the positive solutions of the Volterra-Lotka cooperating model.
文摘In this paper, a kind of multi-level formal concept is introduced. Based on the proposed multi-level formal concept,we present a pair of rough fuzzy set approximations within fuzzy formal contexts.By the proposed rough fuzzy set approximations,we can approximate a fuzzy set according to different precision level.We discuss the properties of the proposed approximation operators in detail.
基金Support by the National Natural Science Foundation of China (30830099)
文摘Objective To explore the feasibility and efficiency of a novel magnetic compression anastomats(MCAs) in intestinal anastomosis.Methods A total of 36 male mongrel canines underwent intestinal anastomosis using traditional hand-sewn(n=18) or a novel MCAs(n=18).We compared the anastomosis time,postoperative complications,bursting strength of anastomoses,gross appearance,and pathology between two groups at each time-point of follow-up.Results The mean anastomosis time with MCAs was significantly less than that with hand-sewn(8.50±1.95 vs.31.1±4.32 minutes,P<0.001).The blood stools and intussusceptions occurred in both groups during follow-up period.Only 1 mongrel canine receiving intestinal anastomosis by MCAs experienced anastomotic leakage.The average bursting pressure of anastomoses obtained from mongrel canines undergoing intestinal anastomosis by MCAs was significantly higher than that by traditional hand-sewn at 1 week's follow-up time(P<0.05).Gross appearance of the anastomoses constructed by MCAs was relatively smoother and flatter.Pathological evalution of anastomoses revealed that general inflammation was greater in hand-sewn anastomoses than magnetic anastomosis.Conclusion The magnetic compression anastomat is a safe and effective device of sutureless intestinal anastomosis in canine models.
基金the National Natural Science Fundation of China (Grant No. 10575079)
文摘The Molten Salt Reactor (MSR) can meet the demand of transmutation and breeding. In this study, theoretical calculation of steady thermal hydraulic characteristics of a graphite-moderated channel type MSR is conducted. The DRAGON code is adopted to calculate the axial and radial power factor firstly. The flow and heat transfer model in the fuel salt and graphite are developed on basis of the fundamental mass, momentum and energy equations. The results show the detailed flow distribution in the core, and the temperature profiles of the fuel salt, inner and outer wall in the nine typical elements along the axial flow direction are also obtained.
基金Project supported by the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(Grant No.51521065)
文摘Chattering phenomenon and singularity are still the main problems that hinder the practical application of sliding mode control. In this paper, a fixed time integral sliding mode controller is designed based on fixed time stability theory, which ensures precise convergence of the state variables of controlled system, and overcomes the drawback of convergence time growing unboundedly as the initial value increases in finite time controller. It makes the controlled system converge to the control objective within a fixed time bounded by a constant as the initial value grows, and convergence time can be changed by adjusting parameters of controllers properly. Compared with other fixed time controllers, the fixed time integral sliding mode controller proposed in this paper achieves chattering-free control, and integral expression is used to avoid singularity generated by derivation. Finally, the controller is used to stabilize four-order chaotic power system. The results demonstrate that the controller realizes the non-singular chattering-free control of chaotic oscillation in the power system and guarantees the fixed time convergence of state variables, which shows its higher superiority than other finite time controllers.
基金Supported by the Key Science and Technology Research Project ofShaanxi Province [2003K10-G35,2004K13-G11(1)].
文摘Objective To construct an expression vector of small interfering RNA （siRNA） against survivin and observe its effects on survivin expression and proliferation of human pancreatic cancer cell line PC-2 and breast cancer cell line MCF-7. Methods Constructed an expression vector of siRNA against survivin and transfected it into PC-2 and MCF-7 cells using lipofectamine^TM 2000. The expression of survivin was detected by semi-quanfifive RT-PCR and immunohistochemistry, and its effects on proliferation of PC-2 and MCF-7 cells were detected by MTT assay. Results The introduction of sequence-specific siRNA could efficiently suppress survivin expression at both mRNA and protein levels in the two cancer cell lines. In PC-2 cells, the expression inhibition rates were 81.25% at mRNA level and 74.24% at protein level In MCF-7 cells, the expression inhibition rates were 64.91% at mRNA level and 79. 72% at protein level The proliferation of PC-2 and MCF-7 cells was also suppressed, and24 and 48 hours after the cells were reseeded, the proliferation inhibition rates of PC-2 cells were 28. 00% and 33. 38%, and that of MCF-7 cells were 31.58% and 33.02%, respectively. Conclusions The expression vector of siRNA against survivin can block survivin expression in PC-2 and MCF-7 cells efficiently and specifically. Down regulation of survivin expression can suppress proliferation of PC-2 and MCF-7 cells. Survivin RNAi may have potential value in gene therapy of human cancers.
基金supported by National High-tech R&D Program of China (No. 2006AA09Z357)the National Science Foundation of China (No. 50876114, No. 10602043)+1 种基金the Program for New Century Excellent Talents in University (NCET-07-0843) and SRF for ROCS, SEMsupported by the State Key Laboratory of Multiphase Flow in Power Engineering (Xi'an Jiaotong University)
文摘In this paper a mathematical model is built for a buried hot crude oil pipeline during shutdown, and an unstructured grid and polar coordinate grid are respectively applied to generating grids for the soil region and the three layers in the pipe （wax layer, pipe wall, and corrosion-inhibiting coating）. The governing equations are discretized using the finite volume method. The variations in temperatures of static oil and soil were investigated during pipeline shutdown in both summer and winter, in which some important parameters of the soil and crude oils of a Northeast pipeline are employed.
文摘Background The objective of this study was to assess the clinical safety and efficacy of vena cava filter （VCF） placement, with particular emphasis on the incidence and risk factors of inferior vena cava thrombosis （VCT） after VCF placement. Methods Clinical data of patients with venous thromboembolism （VTE）, with or without placement of VCF, were analyzed in a retrospective single-center audit of medical records from January 2005 to June 2009. The collected data included demographics, procedural details, filter type, indications, and complications. Results A total of 168 cases of VTE （82 with VCF; 86 without VCF） were examined. Over a median follow-up of 24.2 months, VCT occurred in 18 of 82 patients with VCFs （11 males, 7 females, mean age 55.4 years）. In 86 patients without VCFs, VCT occurred in only 6 individuals （4 males, 2 females） during the study period. VCT was observed more frequently in patients fitted with VCFs than in those without VCFs （22% vs. 7.0%）. Conclusions The incidence of VCT in patients with VTE after VCF implantation was 22% approximately. Anticoagulation therapy should be continued for all patients with VCF placement, unless there is a specific contraindication. Almost all instances of VCT in patients with VCF implants in our study occurred after stopping anticoagulation treatment. The use of VCFs is increasing, and more trials are needed to confirm their benefit and accurately assess their safety.
文摘Drying （conditioning） is an important procedure to prevent hydrate formation during gas pipeline gas-up and to protect pipelines against corrosion. The air-drying method is preferred in offshore gas pipelines pre-commissioning. The air-drying process of gas pipelines commonly includes two steps, air purging and soak test. The mass conservation and the phase equilibrium theory are applied to setting up the mathematical models of air purging, which can be used to simulate dry airflow rate and drying time. Fick diffusion law is applied to setting up the mathematical model of soak test, which can predict the water vapor concentration distribution. The results calculated from the purging model and the soak test model are in good agreement with the experimental data in the DF 1-1 offshore production pipeline conditioning. The models are verified to be available for the air-drying project design of offshore gas pipelines. Some proposals for airdrying engineering and operational procedures are put forward by analyzing the air-drying process of DFI-1 gasexporting pipelines.
基金supported by the Fund of Innovation Research Group of National Natural Science Foundation of China (Grant NO.5052160450323001)Major Program of National Natural Science Foundation of China (Grant No.50536020)
文摘Chokes are one of the most important components of downhole flow-control equipment. The particle erosion mathematical model, which considers particle-particle interaction, was established and used to simulate solid particle movement as well as particle erosion characteristics of the solid-liquid two-phase flow in a choke. The corresponding erosion reduction approach by setting ribs on the inner wall of the choke was advanced. This mathematical model includes three parts： the flow field simulation of the continuous carrier fluid by an Eulerian approach, the particle interaction simulation using the discrete particle hard sphere model by a Lagrangian approach and calculation of erosion rate using semiempirical correlations. The results show that particles accumulated in a narrow region from inlet to outlet of the choke and the dominating factor affecting particle motion is the fluid drag force. As a result, the optimization of rib geometrical parameters indicates that good anti-erosion performance can be achieved by four ribs, each of them with a height （H） of 3 mm and a width （B） of 5 mm equaling the interval between ribs （L）.
基金Supported by National Foundation of Nature Science of China(No.10275063)
文摘Needs in scintimammography applications,especially for small animal cardiac imaging,lead to develop a small field of view,high spatial resolution gamma camera with a pinhole collimator.However the ideal pinhole collimator must keep a compromise between spatial resolution and sensitivity.In order to design a pinhole collimator with an optimized sensitivity and spatial resolution,the spatial resolution and the geometric sensitivity response as a function of the source to collimator distance has been obtained by means of Monte-Carlo simulation for a small field of view gamma camera with a pinhole collimator of various-hole diameters.The results show that the camera with pinhole of 1 mm,1.5 mm and 2 mm diameter has respectively spatial resolution of 1.5 mm,2.25 mm and 3 mm and geometric sensitivity of 0.016%,0.022%and 0.036%,while the source to collimator distance is 3 cm.We chose the pinhole collimator with hole diameter size of 1.2 mm for our the gamma camera designed based on the trade-off between sensitivity and resolution.
基金supported in part by National Basic Research Program of China(973 Project)(No.2014CB239501)National Natural Science Foundation of China(Nos.51707100,51377089)+1 种基金State Key Laboratory of Electrical Insulation and Power Equipment(No.EIPE16208)China Postdoctoral Science Foundation(No.2016M591176)
文摘In this paper,work was conducted to reveal electrical tree behaviors（initiation and propagation）of silicone rubber（SIR） under an impulse voltage with high temperature.Impulse frequencies ranging from 10 Hz to 1 k Hz were applied and the temperature was controlled between 30 °C and 90 °C.Experimental results show that tree initiation voltage decreases with increasing pulse frequency,and the descending amplitude is different in different frequency bands.As the pulse frequency increases,more frequent partial discharges occur in the channel,increasing the tree growth rate and the final shape intensity.As for temperature,the initiation voltage decreases and the tree shape becomes denser as the temperature gets higher.Based on differential scanning calorimetry results,we believe that partial segment relaxation of SIR at high temperature leads to a decrease in the initiation voltage.However,the tree growth rate decreases with increasing temperature.Carbonization deposition in the channel under high temperature was observed under microscope and proven by Raman analysis.Different tree growth models considering tree channel characteristics are proposed.It is believed that increasing the conductivity in the tree channel restrains the partial discharge,holding back the tree growth at high temperature.
基金Project 06JK244 supported by the Special Foundation of Education Department of Shaanxi Province
文摘A thermogravimetric analysis (TG) was conducted to study the thermal decomposition behavior and kinetics of composites from coal and high density polyethylene (HDPE), linear low density polyethylene (LLDPE) or low density polyethylene (LDPE). The results show that coal facilitates melting of the polyethylene before temperatures reach 700 K in nitrogen due to the exothermic effect of coal. Above 700 K, adding coal into the polyethylene will result in smaller maximum rates of mass loss and higher initial mass loss temperatures of the composites. Hence, some chemical interactions, occurring between liquid compounds released in the pyrolysis of the coal and polymer, depend on several factors, such as coal rank and the molecular structure of polymers. Synergetic effects in coal and polymers were also found. Both chemical interactions and synergetic effects control the entire thermal decomposition behavior of compos- ites. The larger the amount of coal in the composites, the greater the decomposition temperature spans and the higher the maximum decomposition temperature, the smaller the devolatilization rates. The effect of coal on the thermal stability of composites lies in the hydrogen acceptor effect of the coals. Thermal decomposition of the coals, the polymers and related composites can be modelled via first order parallel reactions between 563 K and 763 K.
基金Supported by the national key laboratory on Bubble Physics and Natural Circulation (BNPC)the Program for New Century Excellent Talents in University (NCET-06-0837)
文摘The method of characteristic(MOC) was adopted to analyze the check valve-induced water hammer behaviors for a Parallel Pumps Feedwater System(PPFS) during the alternate startup process.The motion of check valve disc was simulated using inertial valve model.Transient parameters including the pressure oscillation,local flow velocity and slamming of the check valve disc etc.have been obtained.The results showed that severe slamming between the valve disc and valve seat occurred during the alternate startup of parallel pumps.The induced maximum pressure vibration amplitude is up to 5.0 MPa.The scheme of appending a damping torque to slow down the check valve closing speed was also performed to mitigate of water hammer.It has been numerically approved to be an effective approach.
基金Subsidized by NSFC(11571274 and 11171269)the Ph.D.Programs Foundation of Ministry of Education of China(20110201110027)
文摘Although the two-grid finite element decoupled scheme for mixed Navier-Stokes/ Darcy model in literatures has given the numerical results of optimal convergence order, the theoretical analysis only obtain the optimal error order for the porous media flow and a non-optimal error order for the fluid flow. In this article, we give a more rigorous of the error analysis for the fluid flow and obtain the optimal error estimates of the velocity and the pressure.
文摘Objective To establish and evaluate a hypercoagulable animal model for the assessment of anticoagulants. Methods Forty mice, thirty-two rats, and twenty-four rabbits were randomly and equally divided into control group (saline) and three ellagic acid (EA)-treated groups (low, middle, and high doses). In the mice, bleeding time (BT) was estimated with tail transaction, and clotting time (CT) with template method. Prothrombin time (PT) and the activated partial thromboplastin time (APTT) in rats and rabbits were measured by means of Quick's one-stage assay and modified APTT assay respectively. In addition, thrombin activity was estimated in rats with PT assay using a hemagglutination analyzer. The circulating platelet aggregates were de- tected in rabbits through platelet counting and presented as the circulating platelet aggregate ratio (CPAR). Results EA shortened BT and CT in mice, PT and APTT in rats, and increased thrombin activity and CPAR, all in a dose-dependent manner. EA also brought reduction of PT and APTT in rabbits in dose- and time-dependent manners. Conclusion EA could induce hypercoagulable state through activating coagulation system and platelets in mice, rats, and rabbits.
基金Supported by a grant from Desert Foundation (2003),Salt Lake City,USA
文摘Objective To investigate the levels of cardiovascular disease risk factors and their relations to clinical phenotype associated with coronary artery disease(CAD).Methods The subjects were recruited from five independent cardiovascular centers.Coronary angiography was employed to define the CAD with stenosis in each major vessel ≥70% and control with stenosis <10% in every lesion.The classic risk factors including family history,body mass index,smoking habits,hypertension,diabetes mellitus,and serum lipid levels were surveyed according to established criteria.Associations between risk levels and clinical phenotypes were assessed by case control and correlation analysis.Results A total of 762 individuals were collected,including 481 men and 281 women,aged from 17 to 81(mean 60±10) years.The patients with CAD accounted for 55.5% of all participants,and controls 44.5%,respectively.Compared with the pattern in published data,our study showed that mean serum high density lipoprotein cholesterol(HDL-C) level was significantly lower(P<0.001) and triglycerides was significantly higher(P<0.001),while total cholesterol(TC) and low density lipoprotein cholesterol levels were comparative(both P>0.05).The prevalence of low HDL-C(<40 g/L) and hypertriglyceridemia(>150 g/L) were 27.2% and 41.4%,respectively.Mean serum levels of HDL-C and apolipoprotein A1 were significantly higher in female subjects than in male(P<0.001).Lower HDL-C functioned as an independent risk factor for CAD only in men(RR=2.8,95%CI:1.5-4.2,P<0.001),yet increased non-HDL cholesterol combined with diabetes mellitus and obesity seemed to play a key role in the development ofCAD in women.Similarity in risk association with CAD was found for hypertension and TC/HDL ratio in male and female subjects,while family history had no relationship with the presence of CAD.Conclusion It is remarkable that emphasis of intervention in future should be given on the prevalent low serum HDL-C and its strong risk correlation with the presence of CAD in male subjects of Chinese Han population.
