OBJECTIVE To study the dysregulation of metallothioneins(MT)and circadian genes in achieved human hepatocellular carcinoma(HCC),Peri-HCC tissues as compared to normal human livers,which may open up a new avenue for ta...OBJECTIVE To study the dysregulation of metallothioneins(MT)and circadian genes in achieved human hepatocellular carcinoma(HCC),Peri-HCC tissues as compared to normal human livers,which may open up a new avenue for targeting circadian clock to prevent and treatment of HCC.METHODS Total RNA was extracted,purified,and reverse transcribed.Realtime quantitative polymerase chain reaction(RT-q PCR)was performed to determine the expression of genes of metallothionein-1(MT-1),MT-2,and metal transcription factor-1(MFT-1)and circadian clock genes including core clock genes,circadian locomotor output cycles kaput(Clock)and brain and muscle Arnt-like protein 1(Bmal1),the clock feedback control genes,period(Per1,Per2)and cryptochrome(Cry1,Cry2),and clock target genes,nuclear orphan receptor factor protein(Nr1d1)and D-box-binding protein(Dbp)in human HCC,Peri-HCC and normal livers.RESULTS The expression of MT-1,MT-2,MFT-1 in human HCC was decreased,which were previously shown to have circadian rhythms.Similarly,the expression of the core clock genes(Bmal1,Clock),the clock feedback genes(Per1,Per2,Cry1and Cry2)was decreased in human HCC,as compared to Peri-HCC and normal livers.However,the expression of clock target genes(Nr1d1and Dbp)was increased in human HCC,as compared to Peri-HCC and normal livers.CONCLUSION These data clearly demonstrated the dysregulation of MTs and circadian clock genes in HCC,which could provide new insights into the relationship of circadian clock disruption and loss of MT in hepatocarcinogenesis,and could help a new strategy of targeting MT and circadian clock in the prevention and treatment of HCC.展开更多
文摘OBJECTIVE To study the dysregulation of metallothioneins(MT)and circadian genes in achieved human hepatocellular carcinoma(HCC),Peri-HCC tissues as compared to normal human livers,which may open up a new avenue for targeting circadian clock to prevent and treatment of HCC.METHODS Total RNA was extracted,purified,and reverse transcribed.Realtime quantitative polymerase chain reaction(RT-q PCR)was performed to determine the expression of genes of metallothionein-1(MT-1),MT-2,and metal transcription factor-1(MFT-1)and circadian clock genes including core clock genes,circadian locomotor output cycles kaput(Clock)and brain and muscle Arnt-like protein 1(Bmal1),the clock feedback control genes,period(Per1,Per2)and cryptochrome(Cry1,Cry2),and clock target genes,nuclear orphan receptor factor protein(Nr1d1)and D-box-binding protein(Dbp)in human HCC,Peri-HCC and normal livers.RESULTS The expression of MT-1,MT-2,MFT-1 in human HCC was decreased,which were previously shown to have circadian rhythms.Similarly,the expression of the core clock genes(Bmal1,Clock),the clock feedback genes(Per1,Per2,Cry1and Cry2)was decreased in human HCC,as compared to Peri-HCC and normal livers.However,the expression of clock target genes(Nr1d1and Dbp)was increased in human HCC,as compared to Peri-HCC and normal livers.CONCLUSION These data clearly demonstrated the dysregulation of MTs and circadian clock genes in HCC,which could provide new insights into the relationship of circadian clock disruption and loss of MT in hepatocarcinogenesis,and could help a new strategy of targeting MT and circadian clock in the prevention and treatment of HCC.
