Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study...Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study was to clarify whether the introduction of Bt gene directly reduces the K-use efficiency of cotton to induce K deficiency.Results:The cotton variety,Jihe 321(wild type,WT)and its two Bt(Cry1Ac)-transgenic overexpression lines(OE-29317,OE-29312)were studied in field with low soil-test K+(47.8 mg·kg^(−1)).In the field with low soil-test K+,only OE-29317 had less biomass and K+accumulation than the WT at some growth stages.Both Bt lines produced similar or even greater seed cotton yield than WT in the field.When the Bt gene(~70%)in OE-29317 and OE-29312 plants was silenced by virus-induced gene silencing(VIGS),the VIGS-Bt plants did not produce more biomass than VIGSgreen fluorescent protein(control)plants.Conclusions:The introduction of Bt gene did not necessarily hinder the K use efficiency of the cotton lines under this study.展开更多
Background:Gossypium hirsutum is highly susceptible to Verticillium wilt,and once infected Verticillium wilt,its yield is greatly reduced.But G.barbadense is highly resistant to Verticillium wilt.It is possible that t...Background:Gossypium hirsutum is highly susceptible to Verticillium wilt,and once infected Verticillium wilt,its yield is greatly reduced.But G.barbadense is highly resistant to Verticillium wilt.It is possible that transferring some disease-resistant genes from G.barbadense to G.hirsutum may contribute to G.hirsutum resistance to Verticillium wilt.Result:Here,we described a new gene in G.barbadense encoding AXMN Toxin Induced Protein-11,Gb At11,which is specifically induced by Verticillium dahliae in G.barbadense and enhances Verticillium wilt resistance in G.hirsutum.Overexpression in G.hirsutum not only significantly improves resistance to Verticillium wilt,but also increases the boll number per plant.Transcriptome analysis and real-time polymerase chain reaction showed that Gb At11 overexpression can simultaneously activate FLS2,BAK1 and other genes,which are involved in ETI and PTI pathways in G.hirsutum.Conclusion:These data suggest that Gb At11 plays a very important role in resistance to Verticillium wilt in cotton.And it is significant for improving resistance to Verticillium wilt and breeding high-yield cotton cultivars.展开更多
文摘Background:Potassium(K)deficiency has become a common field production problem following the widespread adoption of Bacillus thuringiensis(Bt)transgenic cotton(Gossypium hirsutum L.)worldwide.The purpose of this study was to clarify whether the introduction of Bt gene directly reduces the K-use efficiency of cotton to induce K deficiency.Results:The cotton variety,Jihe 321(wild type,WT)and its two Bt(Cry1Ac)-transgenic overexpression lines(OE-29317,OE-29312)were studied in field with low soil-test K+(47.8 mg·kg^(−1)).In the field with low soil-test K+,only OE-29317 had less biomass and K+accumulation than the WT at some growth stages.Both Bt lines produced similar or even greater seed cotton yield than WT in the field.When the Bt gene(~70%)in OE-29317 and OE-29312 plants was silenced by virus-induced gene silencing(VIGS),the VIGS-Bt plants did not produce more biomass than VIGSgreen fluorescent protein(control)plants.Conclusions:The introduction of Bt gene did not necessarily hinder the K use efficiency of the cotton lines under this study.
基金financially supported by the National Key Research and Development Program of China(2016YFD0101904)。
文摘Background:Gossypium hirsutum is highly susceptible to Verticillium wilt,and once infected Verticillium wilt,its yield is greatly reduced.But G.barbadense is highly resistant to Verticillium wilt.It is possible that transferring some disease-resistant genes from G.barbadense to G.hirsutum may contribute to G.hirsutum resistance to Verticillium wilt.Result:Here,we described a new gene in G.barbadense encoding AXMN Toxin Induced Protein-11,Gb At11,which is specifically induced by Verticillium dahliae in G.barbadense and enhances Verticillium wilt resistance in G.hirsutum.Overexpression in G.hirsutum not only significantly improves resistance to Verticillium wilt,but also increases the boll number per plant.Transcriptome analysis and real-time polymerase chain reaction showed that Gb At11 overexpression can simultaneously activate FLS2,BAK1 and other genes,which are involved in ETI and PTI pathways in G.hirsutum.Conclusion:These data suggest that Gb At11 plays a very important role in resistance to Verticillium wilt in cotton.And it is significant for improving resistance to Verticillium wilt and breeding high-yield cotton cultivars.
