Glial precursor cells are a group of glial cells surrounding the sensory neurons in dorsal root ganglia(DRG).In our previous study,we isolated and induced glial precursor cells derived from DRG to adopt a neuronal phe...Glial precursor cells are a group of glial cells surrounding the sensory neurons in dorsal root ganglia(DRG).In our previous study,we isolated and induced glial precursor cells derived from DRG to adopt a neuronal phenotype in vitro.But the RNA level change during the neuronal differentiation of glial precursor cells has not been clarified.In this study,sequencing was performed followed by enrichment analysis employing GO terms and KEGG pathways,and then identified genes were subjected to PPI network analysis during the neuronal differentiation process.Gene expression was verified by qRT-PCR and evaluated after RNA interference.We found that Wnt and neurotrophin signaling pathways were associated with neuronal differentiation by GO terms and KEGG pathways.展开更多
Ischemic stroke(IS)is a major disease threatening human health,and reperfusion therapy are necessary.However,this can cause ischemia/reperfusion(I/R)injury,and the mechanism is complicated.Pyroptosis is an inflammatio...Ischemic stroke(IS)is a major disease threatening human health,and reperfusion therapy are necessary.However,this can cause ischemia/reperfusion(I/R)injury,and the mechanism is complicated.Pyroptosis is an inflammation related programmed cell death mode between necrosis and apoptosis,which plays an essential role in the pathological process of I/R injury.Competitive endogenous RNA(ceRNA)has been proven to be an essential regulatory mechanism in many diseases.展开更多
文摘Glial precursor cells are a group of glial cells surrounding the sensory neurons in dorsal root ganglia(DRG).In our previous study,we isolated and induced glial precursor cells derived from DRG to adopt a neuronal phenotype in vitro.But the RNA level change during the neuronal differentiation of glial precursor cells has not been clarified.In this study,sequencing was performed followed by enrichment analysis employing GO terms and KEGG pathways,and then identified genes were subjected to PPI network analysis during the neuronal differentiation process.Gene expression was verified by qRT-PCR and evaluated after RNA interference.We found that Wnt and neurotrophin signaling pathways were associated with neuronal differentiation by GO terms and KEGG pathways.
文摘Ischemic stroke(IS)is a major disease threatening human health,and reperfusion therapy are necessary.However,this can cause ischemia/reperfusion(I/R)injury,and the mechanism is complicated.Pyroptosis is an inflammation related programmed cell death mode between necrosis and apoptosis,which plays an essential role in the pathological process of I/R injury.Competitive endogenous RNA(ceRNA)has been proven to be an essential regulatory mechanism in many diseases.
