In this work, Staphylococcus epidermidis (S. epidermidis) was used to prepare the fermentation broths with antioxidant activity. Through the optimization of the carbon source, three kinds of S. epidermidis fermentatio...In this work, Staphylococcus epidermidis (S. epidermidis) was used to prepare the fermentation broths with antioxidant activity. Through the optimization of the carbon source, three kinds of S. epidermidis fermentation broth were obtained and designated as SFB, Gly-SFB, and Glu-SFB, which were cultivated in beef protein medium and the beef protein medium supplemented with glycerol or glucose, respectively. The differences in antioxidant efficacy of SFB, Gly-SFB and Glu-SFB were investigated by evaluating intracellular ROS fluorescence intensity, SOD enzyme activity and MDA concentration. Gly-SFB and Glu-SFB exhibited a greater capacity to eliminate ROS as compared to that of SFB. The intracellular SOD enzyme activity increased as the concentrations of SFB and Gly-SFB increased. Nevertheless, the intracellular SOD enzyme activity was the highest after the treatment with Glu-SFB at the low concentrations. The intracellular MDA content reached a lower value after the treatment with Gly-SFB and Glu-SFB at lower concentrations, which was opposite to the case after the treatment with SFB. WB indicated that the S. epidermidis fermentation broth regulated the expression of relevant proteins in the Nrf2-Keap1 signaling pathway to exhibit the antioxidant effects. This indicates that the S. epidermidis fermentation broth promotes the expression of relevant proteins in the Nrf2-Keap1 signaling pathway, consequently, antioxidant benefits were exerted. The fermentation broth that were prepared by incorporating glycerol or glucose into the culture medium can augment their antioxidant activity.展开更多
This study reports the synthesis of oleic acid sterol ester with liquid crystalline properties and its enhanced stability and UV-blocking performance through microencapsulation.Oleic acid sterol ester was synthesized ...This study reports the synthesis of oleic acid sterol ester with liquid crystalline properties and its enhanced stability and UV-blocking performance through microencapsulation.Oleic acid sterol ester was synthesized via the esterification of phytosterol and oleic acid,whose structure was characterized using Fourier-transform infrared spectroscopy(FTIR)and mass spectrometry(MS).Its liquid crystalline behavior was confirmed via the polarized optical microscopy(POM),thermogravimetric analysis(TGA),differential scanning calorimetry(DSC),wide-angle X-ray scattering(WAXS),and small-angle X-ray scattering(SAXS).UV absorption tests were conducted to assess the UV-blocking performance of the oleic acid sterol ester liquid crystals.To improve the stability of its liquid crystalline structure,the oleic acid sterol ester was encapsulated into microcapsules through the emulsion polymerization.SPF measurements were performed on the sunscreen formulations containing liquid crystal microcapsules.The oleic acid sterol ester displayed cholesteric liquid crystalline behavior and strong UVA absorption,which indicates its suitability as a natural UV absorber.Microencapsulation further enhanced its stability and UV-blocking properties.SPF testing showed that the formulations with microcapsules achieved an SPF value of 7.01,which surpasses the nano titanium dioxide(SPF=6.23)and significantly outperform the unencapsulated liquid crystal formulations(SPF=2.65).This study highlights the potential of microencapsulated oleic acid sterol ester as a novel UV absorber in the sunscreen formulations,offers the enhanced stability and effective UV protection,and showcases its application potential in the innovative cosmetic products.展开更多
文摘In this work, Staphylococcus epidermidis (S. epidermidis) was used to prepare the fermentation broths with antioxidant activity. Through the optimization of the carbon source, three kinds of S. epidermidis fermentation broth were obtained and designated as SFB, Gly-SFB, and Glu-SFB, which were cultivated in beef protein medium and the beef protein medium supplemented with glycerol or glucose, respectively. The differences in antioxidant efficacy of SFB, Gly-SFB and Glu-SFB were investigated by evaluating intracellular ROS fluorescence intensity, SOD enzyme activity and MDA concentration. Gly-SFB and Glu-SFB exhibited a greater capacity to eliminate ROS as compared to that of SFB. The intracellular SOD enzyme activity increased as the concentrations of SFB and Gly-SFB increased. Nevertheless, the intracellular SOD enzyme activity was the highest after the treatment with Glu-SFB at the low concentrations. The intracellular MDA content reached a lower value after the treatment with Gly-SFB and Glu-SFB at lower concentrations, which was opposite to the case after the treatment with SFB. WB indicated that the S. epidermidis fermentation broth regulated the expression of relevant proteins in the Nrf2-Keap1 signaling pathway to exhibit the antioxidant effects. This indicates that the S. epidermidis fermentation broth promotes the expression of relevant proteins in the Nrf2-Keap1 signaling pathway, consequently, antioxidant benefits were exerted. The fermentation broth that were prepared by incorporating glycerol or glucose into the culture medium can augment their antioxidant activity.
文摘This study reports the synthesis of oleic acid sterol ester with liquid crystalline properties and its enhanced stability and UV-blocking performance through microencapsulation.Oleic acid sterol ester was synthesized via the esterification of phytosterol and oleic acid,whose structure was characterized using Fourier-transform infrared spectroscopy(FTIR)and mass spectrometry(MS).Its liquid crystalline behavior was confirmed via the polarized optical microscopy(POM),thermogravimetric analysis(TGA),differential scanning calorimetry(DSC),wide-angle X-ray scattering(WAXS),and small-angle X-ray scattering(SAXS).UV absorption tests were conducted to assess the UV-blocking performance of the oleic acid sterol ester liquid crystals.To improve the stability of its liquid crystalline structure,the oleic acid sterol ester was encapsulated into microcapsules through the emulsion polymerization.SPF measurements were performed on the sunscreen formulations containing liquid crystal microcapsules.The oleic acid sterol ester displayed cholesteric liquid crystalline behavior and strong UVA absorption,which indicates its suitability as a natural UV absorber.Microencapsulation further enhanced its stability and UV-blocking properties.SPF testing showed that the formulations with microcapsules achieved an SPF value of 7.01,which surpasses the nano titanium dioxide(SPF=6.23)and significantly outperform the unencapsulated liquid crystal formulations(SPF=2.65).This study highlights the potential of microencapsulated oleic acid sterol ester as a novel UV absorber in the sunscreen formulations,offers the enhanced stability and effective UV protection,and showcases its application potential in the innovative cosmetic products.
