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Simultaneous identification of multiple animal-derived components in meat and meat products by using MNP marker based on high-throughput sequencing
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作者 Yan Yi Zhanyue Jiang +9 位作者 Lixia Ma Xiaoni Hou Lun Li Deping Ye Juanlan Du Hai Peng Guoquan Han Huaiping Li Jiangwen Tang Lihua Zhou 《Food Science and Human Wellness》 2025年第4期1566-1575,共10页
In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology bas... In order to save manpower and time costs,and to achieve simultaneous detection of multiple animal-derived components in meat and meat products,this study used multiple nucleotide polymorphism(MNP)marker technology based on the principle of high-throughput sequencing,and established a multi-locus 10 animalderived components identification method of cattle,goat,sheep,donkey,horse,chicken,duck,goose,pigeon,quail in meat and meat products.The specific loci of each species could be detected and the species could be accurately identified,including 5 loci for cattle and duck,3 loci for sheep,9 loci for chicken and horse,10 loci for goose and pigeon,6 loci for quail and 1 locus for donkey and goat,and an adulteration model was established to simulate commercially available samples.The results showed that the method established in this study had high throughput,good repeatability and accuracy,and was able to identify 10 animalderived components simultaneously with 100%repeatability accuracy.The detection limit was 0.1%(m/m)in simulated samples of chicken,duck and horse.Using the method established in this study to test commercially available samples,4 samples from 14 commercially available samples were detected to be inconsistent with the labels,of which 2 did not contain the target ingredient and 2 were adulterated with small amounts of other ingredients. 展开更多
关键词 Meat and meat products Multiple nucleotide polymorphism marker method High-throughput sequencing Animal-derived component identification
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Measurement of air kerma rate and ambient dose equivalent rate using the G(E) function with hemispherical CdZnTe detector
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作者 Ping Huang 《Nuclear Science and Techniques》 SCIE CAS CSCD 2018年第3期71-77,共7页
Since the room-temperature detector CdZnTe(CZT) has advantages in terms of detection efficiency,energy resolution, and size, it has been extensively used to detect X-rays and gamma-rays. So far, nuclear radiation dete... Since the room-temperature detector CdZnTe(CZT) has advantages in terms of detection efficiency,energy resolution, and size, it has been extensively used to detect X-rays and gamma-rays. So far, nuclear radiation detectors such as cerium chloride doped with lanthanum bromide(LaBr_3(Ce)), thallium doped with cesium iodide(sI(Tl)), thallium doped with sodium iodide(NaI(Tl)),and high-purity germanium(HPGe) primarily use the spectroscopy-dose rate function(G(E)) to achieve the accurate measurement of air kerma rate(K_a) and ambient dose equivalent rate(H*(10)). However, the spectroscopy-dose rate function has been rarely measured for a CZT detector. In this study, we performed spectrum measurement using a hemispherical CZT detector in a radiation protection standards laboratory. The spectroscopy-dose rate function G(E) of the CZT detector was calculated using the least-squares method combined with the standard dose rate at the measurement position. The results showed that the hemispherical CZT detector could complete the measurement of air kerma rate(K_a) and ambient dose equivalent rate(H*(10)) by using the G(E) function at energies between 48 keV and 1.25 MeV, and the relative intrinsic errors were, respectively, controlled within ± 2. 3 and ± 2. 1%. 展开更多
关键词 CDZNTE Spectroscopy-dose RATE G(E) FUNCTION Air kerma RATE AMBIENT DOSE equivalent RATE Relative intrinsic error
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Photon energy response optimization using few-channel spectroscopy dose method for Si-PIN photodetector applied in personal dose equivalent measurements 被引量:1
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作者 Ping Huang Shu-Qiong Li +3 位作者 Run-Dong Yang Yun-Tian Sun Yong Yang Wu Gao 《Nuclear Science and Techniques》 SCIE CAS CSCD 2020年第4期37-46,共10页
Si-PIN photodetectors having features such as low cost,small size,low weight,low voltage,and low power consumption are widely used as radiation detectors in electronic personal dosimeters(EPDs).The technical parameter... Si-PIN photodetectors having features such as low cost,small size,low weight,low voltage,and low power consumption are widely used as radiation detectors in electronic personal dosimeters(EPDs).The technical parameters of EPDs based on the Si-PIN photodetectors include photon energy response(PER),angular response,inherent error,and dose rate linearity.Among them,PER is a key parameter for evaluation of EPD measurement accuracy.At present,owing to the limitations of volume,power consumption,and EPD cost,the PER is usually corrected by a combination of single-channel counting techniques and filtering material methods.However,the above-mentioned methods have problems such as poor PER and low measurement accuracy.To solve such problems,in this study,a 1024-channel spectrometry system using a Si-PIN photodetector was developed and fullspectrum measurement in the reference radiation fields was conducted for radiation protection.The measurement results using the few-channel spectroscopy dose method showed that the PER could be controlled within±14%and±2%under the conditions of two and three energy intervals,respectively,with different channel numbers.The PER measured at 0°angle of radiation incidence meets the-29%to+67%requirements of IEC 61526:2010.Meanwhile,the channel number and counts-to-dose conversion factors formed in the experiment can be integrated into an EPD. 展开更多
关键词 Electronic PERSONAL dosimeter Si-PIN PHOTODETECTOR PERSONAL DOSE equivalent Photon energy response Few-channel SPECTROSCOPY DOSE method
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Scanning transmission proton microscopy tomography of reconstruction cells from simulated data
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作者 ZHANG Conghua LI Min HOU Qing 《Nuclear Science and Techniques》 SCIE CAS CSCD 2011年第2期89-94,共6页
For scanning transmission proton microscopy tomography,to compare cell images of the proton stopping power and relative electron density,two cell phantoms are designed and simulated by code FLUKA.The cell images are r... For scanning transmission proton microscopy tomography,to compare cell images of the proton stopping power and relative electron density,two cell phantoms are designed and simulated by code FLUKA.The cell images are reconstructed by the filtered back projection algorithm,and compared with their tomography imaging.The images of stopping power and relative electron density slightly vary with proton energies,but the internal images are of clear with high resolution.The organic glass image of relative electron density reveals the resolution power of proton tomography.Also,the simulation results reflect effects of the boundary enhancement,the weak artifacts,and the internal structure border extension by multiple scattering.So using proton tomography to analyze internal structure of a cell is a superior. 展开更多
关键词 细胞图像 断层扫描 显微成像 质子 模拟 数据传输 滤波反投影算法 电子密度
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Energy response correction for an electronic personal dosimeter using the channel ratio method
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作者 Ping Huang You-De Zhang +3 位作者 Yong Yang Cong-Hua Zhang Lan Gong Qian Yang 《Nuclear Science and Techniques》 SCIE CAS CSCD 2017年第7期65-72,共8页
An electronic personal dosimeter mainly uses a Si-PIN photodiode as X-and gamma-ray detectors.The photon energy response of this instrument is inconsistent in the case of no correction,which seriously affects the accu... An electronic personal dosimeter mainly uses a Si-PIN photodiode as X-and gamma-ray detectors.The photon energy response of this instrument is inconsistent in the case of no correction,which seriously affects the accurate monitoring of personal dose equivalent H_p(10)parameters for radiation workers.For this reason,in this paper we propose a method of combining composite screen detection technology,multichannel measurement technology,and the channel ratio method to achieve accurate measurement of the personal dose equivalent parameters.According to China National Standard GB/T 13161-2003 and National Verification Regulation JJG 1009-2006,the instrument was tested in the energy range between 48 keV and 1.25 MeV.The experimental results showed that the difference of energy response to ^(137)C_S corrected by the new method was almost constant within ±6.0%,which fulfilled the ±30% requirement of GB/T 13161-2003 and JJG1009-2006.Meanwhile,the method proposed obtained energy information regarding the radiation field. 展开更多
关键词 能量响应 多通道 个人剂量计 正电子 PIN光电二极管 个人剂量当量 电子个人剂量仪 Γ射线探测器
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