Background:Soil salt stress seriously restricts the yield and quality of cotton worldwide.To investigate the molecular mechanism of cotton response to salt stress,a main cultivated variety Gossypium hirsutum L.acc.Xin...Background:Soil salt stress seriously restricts the yield and quality of cotton worldwide.To investigate the molecular mechanism of cotton response to salt stress,a main cultivated variety Gossypium hirsutum L.acc.Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis.Results:Through transcriptome analysis in cotton leaves under salt stress for 0 h(T0),3 h(T3)and 12 h(T12),we identified 8436,11628 and 6311 differentially expressed genes(DEGs)in T3 vs.T0,T12 vs.T0 and T12 vs.T3,respectively.A total of 459 differentially expressed proteins(DEPs)were identified by proteomic analysis,of which 273,99 and 260 DEPs were identified in T3 vs.T0,T12 vs.T0 and T12 vs.T3,respectively.Metabolic pathways,biosynthesis of secondary metabolites,photosynthesis and plant hormone signal transduction were enriched among the identified DEGs or DEPs.Detail analysis of the DEGs or DEPs revealed that complex signaling pathways,such as abscisic acid(ABA)and jasmonic acid(JA)signaling,calcium signaling,mitogen-activated protein kinase(MAPK)signaling cascade,transcription factors,activation of antioxidant and ion transporters,were participated in regulating salt response in cotton.Conclusions:Our research not only contributed to understand the mechanism of cotton response to salt stress,but also identified nine candidate genes,which might be useful for molecular breeding to improve salt-toleranee in cotton.展开更多
基金This work was supported by National R&D Project of Transgenic Crops of Ministry of Science and Technology of China(2016ZX08005–004-002).
文摘Background:Soil salt stress seriously restricts the yield and quality of cotton worldwide.To investigate the molecular mechanism of cotton response to salt stress,a main cultivated variety Gossypium hirsutum L.acc.Xinluzhong 54 was used to perform transcriptome and proteome integrated analysis.Results:Through transcriptome analysis in cotton leaves under salt stress for 0 h(T0),3 h(T3)and 12 h(T12),we identified 8436,11628 and 6311 differentially expressed genes(DEGs)in T3 vs.T0,T12 vs.T0 and T12 vs.T3,respectively.A total of 459 differentially expressed proteins(DEPs)were identified by proteomic analysis,of which 273,99 and 260 DEPs were identified in T3 vs.T0,T12 vs.T0 and T12 vs.T3,respectively.Metabolic pathways,biosynthesis of secondary metabolites,photosynthesis and plant hormone signal transduction were enriched among the identified DEGs or DEPs.Detail analysis of the DEGs or DEPs revealed that complex signaling pathways,such as abscisic acid(ABA)and jasmonic acid(JA)signaling,calcium signaling,mitogen-activated protein kinase(MAPK)signaling cascade,transcription factors,activation of antioxidant and ion transporters,were participated in regulating salt response in cotton.Conclusions:Our research not only contributed to understand the mechanism of cotton response to salt stress,but also identified nine candidate genes,which might be useful for molecular breeding to improve salt-toleranee in cotton.
