Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH...Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ℃) . Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R=40.0,42.4, 48.0, 62.8, 107.0).. The results indicated that the most important factor was the nature of the strain (R= 107.0),. while the change in temperature and time of cultivation produced the lowest effect (R= 40.0).. Each factor variance was significant and A 3B 4C 2D 1E 3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most.展开更多
Trichoderma aureoviride mutant T1010 was cultured in media containing different nutrients. Statistics showed significant differences and the results described below: The C/N tested were 3∶1, 6∶1, 12∶1, 24∶1,48∶1....Trichoderma aureoviride mutant T1010 was cultured in media containing different nutrients. Statistics showed significant differences and the results described below: The C/N tested were 3∶1, 6∶1, 12∶1, 24∶1,48∶1. The best C/N was 24∶1. The amount of spores was 4.0×109 cfu/plate. The best carbon source among glucose, sucrose, maltose, lactose, cellulose and starch was the last one. With a starch-containing medium, T1010 produced 5.3×109 cfu/plate. The asparagine was the best nitrogen source. T1010 absorbed it easily and produced 9.6×109 cfu/plate. K, Mg, P, S, Fe were important to support T1010 growth and sporulation. In the absence of K, P, and Fe, Trichoderma grew poorly, while Mg didn’t seem to help Trichoderma colony development. A little amount of vitamins allwed T1010 to grow better. This data allowed us to improve the cultivation of Trichoderma at industrial level.展开更多
文摘Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ℃) . Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R=40.0,42.4, 48.0, 62.8, 107.0).. The results indicated that the most important factor was the nature of the strain (R= 107.0),. while the change in temperature and time of cultivation produced the lowest effect (R= 40.0).. Each factor variance was significant and A 3B 4C 2D 1E 3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most.
文摘Trichoderma aureoviride mutant T1010 was cultured in media containing different nutrients. Statistics showed significant differences and the results described below: The C/N tested were 3∶1, 6∶1, 12∶1, 24∶1,48∶1. The best C/N was 24∶1. The amount of spores was 4.0×109 cfu/plate. The best carbon source among glucose, sucrose, maltose, lactose, cellulose and starch was the last one. With a starch-containing medium, T1010 produced 5.3×109 cfu/plate. The asparagine was the best nitrogen source. T1010 absorbed it easily and produced 9.6×109 cfu/plate. K, Mg, P, S, Fe were important to support T1010 growth and sporulation. In the absence of K, P, and Fe, Trichoderma grew poorly, while Mg didn’t seem to help Trichoderma colony development. A little amount of vitamins allwed T1010 to grow better. This data allowed us to improve the cultivation of Trichoderma at industrial level.
