为确定蛋白磷酸酶-1和-2A(protein phosphatase-1 and phosphatase-2A,PP-1,PP-2A)在水生低等脊椎动物眼球不同组织/细胞中的表达模式,提取金鱼眼球中4种组织视网膜、晶体上皮细胞、晶体纤维和角膜的RNA和蛋白,利用RT-PCR和Western印迹...为确定蛋白磷酸酶-1和-2A(protein phosphatase-1 and phosphatase-2A,PP-1,PP-2A)在水生低等脊椎动物眼球不同组织/细胞中的表达模式,提取金鱼眼球中4种组织视网膜、晶体上皮细胞、晶体纤维和角膜的RNA和蛋白,利用RT-PCR和Western印迹技术进行检测.同时运用荧光免疫组织化学技术进行了定位表达研究.结果表明:1)在mRNA水平上,PP-1催化亚基α和PP-2A催化亚基β在金鱼视网膜、晶体上皮和角膜中表达强烈,与其在高等哺乳类脊椎动物如小鼠中的表达形成鲜明的对比;2)在蛋白水平上,PP-1和PP-2A催化亚基在金鱼视网膜和晶体上皮细胞中的表达较高,而在角膜中的表达最低.这与它们在小鼠眼球视网膜和角膜中同时具备高表达的模式形成对比;3)在金鱼发育48h的眼球中,PP-1和PP-2A催化亚基定位于视网膜的色素视网膜和神经视网膜细胞中,晶体上皮和纤维细胞中及角膜上皮细胞中,而在性成熟金鱼眼球中,此二酶催化亚基主要定位于视网膜的色素视网膜,神经视网膜及晶体上皮细胞中.这些结果为探讨PP-1和PP-2A在金鱼眼球不同组织中的功能打下了重要基础。展开更多
Age-related macular degeneration(AMD) is a complex disease with multiple risk factors.Although the pathogenesis and etiology remain largely unclear,systematic investigation into the genetics of this disease has cumula...Age-related macular degeneration(AMD) is a complex disease with multiple risk factors.Although the pathogenesis and etiology remain largely unclear,systematic investigation into the genetics of this disease has cumulated during the past two decades that leads to putative mechanisms and genes associated with AMD.In this review,the development of genetic studies on AMD was summarized.While we intend to perform a comprehensive citation of all published works on AMD genetics,we find that we have to filter out some reference papers and focus on those reports bearing more information and insight into AMD genetics and its molecular pathology.We have worked on a selection of observations and supporting references that illustrate a picture of the genetic processes that eventually lead to the development of AMD and related complications.展开更多
文摘为确定蛋白磷酸酶-1和-2A(protein phosphatase-1 and phosphatase-2A,PP-1,PP-2A)在水生低等脊椎动物眼球不同组织/细胞中的表达模式,提取金鱼眼球中4种组织视网膜、晶体上皮细胞、晶体纤维和角膜的RNA和蛋白,利用RT-PCR和Western印迹技术进行检测.同时运用荧光免疫组织化学技术进行了定位表达研究.结果表明:1)在mRNA水平上,PP-1催化亚基α和PP-2A催化亚基β在金鱼视网膜、晶体上皮和角膜中表达强烈,与其在高等哺乳类脊椎动物如小鼠中的表达形成鲜明的对比;2)在蛋白水平上,PP-1和PP-2A催化亚基在金鱼视网膜和晶体上皮细胞中的表达较高,而在角膜中的表达最低.这与它们在小鼠眼球视网膜和角膜中同时具备高表达的模式形成对比;3)在金鱼发育48h的眼球中,PP-1和PP-2A催化亚基定位于视网膜的色素视网膜和神经视网膜细胞中,晶体上皮和纤维细胞中及角膜上皮细胞中,而在性成熟金鱼眼球中,此二酶催化亚基主要定位于视网膜的色素视网膜,神经视网膜及晶体上皮细胞中.这些结果为探讨PP-1和PP-2A在金鱼眼球不同组织中的功能打下了重要基础。
文摘Age-related macular degeneration(AMD) is a complex disease with multiple risk factors.Although the pathogenesis and etiology remain largely unclear,systematic investigation into the genetics of this disease has cumulated during the past two decades that leads to putative mechanisms and genes associated with AMD.In this review,the development of genetic studies on AMD was summarized.While we intend to perform a comprehensive citation of all published works on AMD genetics,we find that we have to filter out some reference papers and focus on those reports bearing more information and insight into AMD genetics and its molecular pathology.We have worked on a selection of observations and supporting references that illustrate a picture of the genetic processes that eventually lead to the development of AMD and related complications.
