Chromatographic method was invented about 80 years ago.Since thengreat development has been made and it is now widely used as an analyti-cal tool in chemistry,biology,medicine and environmental sciences.Since 1970,hig...Chromatographic method was invented about 80 years ago.Since thengreat development has been made and it is now widely used as an analyti-cal tool in chemistry,biology,medicine and environmental sciences.Since 1970,highly precise HPLC(high performance liquid chromatography)pumps,various kinds of columns and high sensitive detectors have beenmanufactured.The computerizing equipment can implement the automaticanalysis under optimal conditions and provide more precise and convenientdata acquisition and processing.The HPLC technique has become moreand more important for the biologists and medical scientists in展开更多
ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused....ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused. The solvent System was petroleum ether-ethyl acetate-glacial acetic acid (8o:20:1) and the spra-ying reagent was a solution of sulphuric acid and vanillin. Under the selected con-ditions, the peak area was linearly related to the cholesterol amount for the range between 80～700 ng per spot. The intraplate and interplate coefficients were 2.4% and 7.4% respectively. The recovery of cholesterol was 101.6%. The method presented was simple, rapid and accurate. The results of experi-mental investigation and clinical application were satisfactory.展开更多
A high-performance liquid chromatography was describ-ed to measure the concentration of human 3-methylhistidine in urine witho-phthaldialdehyde pre-column derivatization.This assay system resultedin a clear separation...A high-performance liquid chromatography was describ-ed to measure the concentration of human 3-methylhistidine in urine witho-phthaldialdehyde pre-column derivatization.This assay system resultedin a clear separation of 3-methylhistidine derivative from other derivativ-es in urine within 25 min.Linearity,recovery and coefficients of vari-ation were studied.The method has been successfully applied to routineanalysis for clinical studies and diagnosis.展开更多
文摘Chromatographic method was invented about 80 years ago.Since thengreat development has been made and it is now widely used as an analyti-cal tool in chemistry,biology,medicine and environmental sciences.Since 1970,highly precise HPLC(high performance liquid chromatography)pumps,various kinds of columns and high sensitive detectors have beenmanufactured.The computerizing equipment can implement the automaticanalysis under optimal conditions and provide more precise and convenientdata acquisition and processing.The HPLC technique has become moreand more important for the biologists and medical scientists in
文摘ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused. The solvent System was petroleum ether-ethyl acetate-glacial acetic acid (8o:20:1) and the spra-ying reagent was a solution of sulphuric acid and vanillin. Under the selected con-ditions, the peak area was linearly related to the cholesterol amount for the range between 80～700 ng per spot. The intraplate and interplate coefficients were 2.4% and 7.4% respectively. The recovery of cholesterol was 101.6%. The method presented was simple, rapid and accurate. The results of experi-mental investigation and clinical application were satisfactory.
文摘A high-performance liquid chromatography was describ-ed to measure the concentration of human 3-methylhistidine in urine witho-phthaldialdehyde pre-column derivatization.This assay system resultedin a clear separation of 3-methylhistidine derivative from other derivativ-es in urine within 25 min.Linearity,recovery and coefficients of vari-ation were studied.The method has been successfully applied to routineanalysis for clinical studies and diagnosis.
