Background Traffic accidents,anti-terrorism,gas and chemical dangerous goods explosions,earthquake shock wave damage,and falling impacts in daily life and other events involving impact loads cause great harm to human ...Background Traffic accidents,anti-terrorism,gas and chemical dangerous goods explosions,earthquake shock wave damage,and falling impacts in daily life and other events involving impact loads cause great harm to human organs and tissues,and even life-threatening.Such injuries are called impact damage.Although during the previous wars,the treatment of impact injuries has been greatly improved,and its treatment has been widely used in clinical practice.However,under the current development of society,the impact damage incident has not only been limited to the battlefield.Extreme organizations,frequent industrial production accidents,aircraft trains and other accidents have extended the impact damage incidents into daily society,seriously jeopardizing the health of civilians.Therefore,in order to better treat the injured organs under the impact load,such as the reconstruction and recovery of organ tissues,it is necessary to establish a corresponding system of clinical treatment methods for impact damage.In vitro models of traumatic injury are helping elucidate the pathobiological mechanisms responsible for dysfunction and delayed cell functional variation after mechanical stimulation of the single waveform pressure.It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as amplitudes and durations.However,the influence of impulsive pressure on endothelial function has not yet been fully studied in vitro.In this study,we developed a pressure loading device that produced positive by modifying an in vitro fluid percussion model and examined the effects of the pressures on macrophages’basic functions.Methods To model variations in the biomechanical injury parameters and simplify the experiment,single-use syringe was chosen to be the cell container and a drop hammer driven fluid percussion injury system(FPI)was designed and built to generate a single waveform with adjustable peak pressure and durations.Mice macrophage cells(Raw 264.7)were subjected to three types of the single positive pressure(120 kPa,550 kPa and 1 100 kPa).Every 12 hours we detected its basic functions(including phagocytosis and proliferative capacity)during the following 48 hours,also the immediate cell death.Results This single waveform pressure loading device could produce positive pressure with amplitudes of 70~1 200 kPa.After the pressure loading,there is no significant differences between the control cells and experiment cells.However,it does have a notable effect on its basic functions.The results showed that its phagocytosis and proliferative capacity were getting increased with a peak value on36 h and suddenly decreasing on 48 h.Moreover,these 4 regular curves are in proportion to the pressure.And the experimental results also indicate that the cell impact platform can achieve a single impact loading on the cells.The impact mainly causes the functional changes of RAW264.7 cells instead of directly causing its death.The cell proliferation activity and phagocytosis function are enhanced to some extent.Conclusions Those results indicate that single waveform pressure have a main effect on cell’s biological functions,not on cell death.And these effects on functions did have a regular functional rela-tionship.To explore more regular curves and the mechanism,we need more experiments such as genomics technical.展开更多
基金financially supported by the National Natural Science Foundation of China ( 11722220, 11672246,11472224)
文摘Background Traffic accidents,anti-terrorism,gas and chemical dangerous goods explosions,earthquake shock wave damage,and falling impacts in daily life and other events involving impact loads cause great harm to human organs and tissues,and even life-threatening.Such injuries are called impact damage.Although during the previous wars,the treatment of impact injuries has been greatly improved,and its treatment has been widely used in clinical practice.However,under the current development of society,the impact damage incident has not only been limited to the battlefield.Extreme organizations,frequent industrial production accidents,aircraft trains and other accidents have extended the impact damage incidents into daily society,seriously jeopardizing the health of civilians.Therefore,in order to better treat the injured organs under the impact load,such as the reconstruction and recovery of organ tissues,it is necessary to establish a corresponding system of clinical treatment methods for impact damage.In vitro models of traumatic injury are helping elucidate the pathobiological mechanisms responsible for dysfunction and delayed cell functional variation after mechanical stimulation of the single waveform pressure.It is likely that injury outcome is related to the biomechanical parameters of the traumatic event such as amplitudes and durations.However,the influence of impulsive pressure on endothelial function has not yet been fully studied in vitro.In this study,we developed a pressure loading device that produced positive by modifying an in vitro fluid percussion model and examined the effects of the pressures on macrophages’basic functions.Methods To model variations in the biomechanical injury parameters and simplify the experiment,single-use syringe was chosen to be the cell container and a drop hammer driven fluid percussion injury system(FPI)was designed and built to generate a single waveform with adjustable peak pressure and durations.Mice macrophage cells(Raw 264.7)were subjected to three types of the single positive pressure(120 kPa,550 kPa and 1 100 kPa).Every 12 hours we detected its basic functions(including phagocytosis and proliferative capacity)during the following 48 hours,also the immediate cell death.Results This single waveform pressure loading device could produce positive pressure with amplitudes of 70~1 200 kPa.After the pressure loading,there is no significant differences between the control cells and experiment cells.However,it does have a notable effect on its basic functions.The results showed that its phagocytosis and proliferative capacity were getting increased with a peak value on36 h and suddenly decreasing on 48 h.Moreover,these 4 regular curves are in proportion to the pressure.And the experimental results also indicate that the cell impact platform can achieve a single impact loading on the cells.The impact mainly causes the functional changes of RAW264.7 cells instead of directly causing its death.The cell proliferation activity and phagocytosis function are enhanced to some extent.Conclusions Those results indicate that single waveform pressure have a main effect on cell’s biological functions,not on cell death.And these effects on functions did have a regular functional rela-tionship.To explore more regular curves and the mechanism,we need more experiments such as genomics technical.
