An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branch...An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A fourfactor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L-1 6-BA, 0.3 mg · L-1 IBA and 1.5 mg · L-1 GA3.展开更多
基金Supported by the State Bureau of Foreign Experts 2013 Annual Talent Introduction of Foreign Technology,Project Management(20132300046)
文摘An excellent Lonicera edulis strain, L1-8 that was bred by Northeast Institute of Geography and Agro-ecology, Chinese Academy of Sciences, was used as material in this research. The axillary buds of its dormant branches were used as explants. A fourfactor and four-level orthogonal test was designed in order to choose the best differentiation medium for providing the technical support of Lonicera edulis micropropagation. The results showed that the culture medium and concentration of 6-BA were the main factors, and the best differentiation condition was MS culture medium containing 2.0 mg · L-1 6-BA, 0.3 mg · L-1 IBA and 1.5 mg · L-1 GA3.
