[目的]以京尼平苷为原料,在自然pH下,通过产β-葡萄糖苷酶的邬衣黑曲霉发酵将京尼平苷转化为京尼平。[方法]通过单因素和正交试验考察了京尼平苷液浓度、发酵温度、发酵时间、邬衣黑曲霉孢子的接入量、摇瓶装量对京尼平生成量的影响。[...[目的]以京尼平苷为原料,在自然pH下,通过产β-葡萄糖苷酶的邬衣黑曲霉发酵将京尼平苷转化为京尼平。[方法]通过单因素和正交试验考察了京尼平苷液浓度、发酵温度、发酵时间、邬衣黑曲霉孢子的接入量、摇瓶装量对京尼平生成量的影响。[结果]最终确定最佳工艺为:在自然pH条件下,以0.20%京尼平苷液为发酵培养基,按菌株孢子与发酵培养基3.0∶10.0(V/V)的比例接入邬衣黑曲霉孢子,摇瓶装量为100 m L/250 m L,28℃,200 r/min发酵66 h。发酵结束后高效液相色谱检测京尼平,结果显示京尼平苷的转化率可达99%以上,转化产物京尼平纯度可达98%以上。[结论]该研究所用方法安全、高效,是生产京尼平的可行方法。展开更多
[目的]获得大孔吸附树脂法纯化红皮云杉多酚的最佳工艺条件。[方法]通过静态试验筛选大孔吸附树脂,然后再进行动态试验,通过单因素和正交试验,得到纯化红皮云杉多酚的最佳工艺条件。[结果]以D-101为固定相介质,红皮云杉多酚的最佳纯化...[目的]获得大孔吸附树脂法纯化红皮云杉多酚的最佳工艺条件。[方法]通过静态试验筛选大孔吸附树脂,然后再进行动态试验,通过单因素和正交试验,得到纯化红皮云杉多酚的最佳工艺条件。[结果]以D-101为固定相介质,红皮云杉多酚的最佳纯化工艺条件为上样浓度1.5 mg/m L、上样量25 m L、径长比1∶25、洗脱剂浓度70%、洗脱流速2.0 m L/min,多酚得率为56.88%。[结论]该研究为红皮云杉的开发利用提供了理论依据。展开更多
Six coordination polymers based on 9,10-di(pyridine-4-yl)-anthracene(DPA)and 1,6-di(1H-imidazol-1-yl)pyrene(DIP)were obtained by solvothermal reactions.{[Zn(DPA)Cl_(2)]·DMF·2H_(2)O}n(1)and{[Zn_(1.5)(DPA)_(1....Six coordination polymers based on 9,10-di(pyridine-4-yl)-anthracene(DPA)and 1,6-di(1H-imidazol-1-yl)pyrene(DIP)were obtained by solvothermal reactions.{[Zn(DPA)Cl_(2)]·DMF·2H_(2)O}n(1)and{[Zn_(1.5)(DPA)_(1.5)Cl_(3)]·5H_(2)O}n(2)are framework isomers,which both contain zigzag chains formed by DPA,Zn^(2+),and Cl-.The zigzag chains in 1 are further assembled by C—H…Cl interactions into layers,and these layers exhibit two different orientations,displaying a rare 2D to 3D interpenetration mode.The zigzag chains in 2 are parallelly arranged.{[Zn_(3)(DPA)_(3)Br_(6)]·2DMF·_(1.5)H_(2)O}n(3)is isostructural to 2.3 was obtained using ZnBr_(2)instead of ZnCl_(2).[M(DPA)(formate)_(2)(H_(2)O)_(2)]n[M=Co(4),Cu(5)]are isostructural,contain chain structures formed by DPA,Cu^(2+)/Co^(2+),and for-mate ions,which were formed in situ in the solvothermal reaction.{[Zn(DIP)_(2)Cl]ClO_(4)}n(6)contains a layer structure formed by DIP and Zn^(2+).Free DPA and DIP ligands exhibited high fluorescence at room temperature,and coordina-tion polymers 3 and 6 displayed enhanced fluorescent emissions.展开更多
文摘[目的]以京尼平苷为原料,在自然pH下,通过产β-葡萄糖苷酶的邬衣黑曲霉发酵将京尼平苷转化为京尼平。[方法]通过单因素和正交试验考察了京尼平苷液浓度、发酵温度、发酵时间、邬衣黑曲霉孢子的接入量、摇瓶装量对京尼平生成量的影响。[结果]最终确定最佳工艺为:在自然pH条件下,以0.20%京尼平苷液为发酵培养基,按菌株孢子与发酵培养基3.0∶10.0(V/V)的比例接入邬衣黑曲霉孢子,摇瓶装量为100 m L/250 m L,28℃,200 r/min发酵66 h。发酵结束后高效液相色谱检测京尼平,结果显示京尼平苷的转化率可达99%以上,转化产物京尼平纯度可达98%以上。[结论]该研究所用方法安全、高效,是生产京尼平的可行方法。
文摘[目的]获得大孔吸附树脂法纯化红皮云杉多酚的最佳工艺条件。[方法]通过静态试验筛选大孔吸附树脂,然后再进行动态试验,通过单因素和正交试验,得到纯化红皮云杉多酚的最佳工艺条件。[结果]以D-101为固定相介质,红皮云杉多酚的最佳纯化工艺条件为上样浓度1.5 mg/m L、上样量25 m L、径长比1∶25、洗脱剂浓度70%、洗脱流速2.0 m L/min,多酚得率为56.88%。[结论]该研究为红皮云杉的开发利用提供了理论依据。
文摘Six coordination polymers based on 9,10-di(pyridine-4-yl)-anthracene(DPA)and 1,6-di(1H-imidazol-1-yl)pyrene(DIP)were obtained by solvothermal reactions.{[Zn(DPA)Cl_(2)]·DMF·2H_(2)O}n(1)and{[Zn_(1.5)(DPA)_(1.5)Cl_(3)]·5H_(2)O}n(2)are framework isomers,which both contain zigzag chains formed by DPA,Zn^(2+),and Cl-.The zigzag chains in 1 are further assembled by C—H…Cl interactions into layers,and these layers exhibit two different orientations,displaying a rare 2D to 3D interpenetration mode.The zigzag chains in 2 are parallelly arranged.{[Zn_(3)(DPA)_(3)Br_(6)]·2DMF·_(1.5)H_(2)O}n(3)is isostructural to 2.3 was obtained using ZnBr_(2)instead of ZnCl_(2).[M(DPA)(formate)_(2)(H_(2)O)_(2)]n[M=Co(4),Cu(5)]are isostructural,contain chain structures formed by DPA,Cu^(2+)/Co^(2+),and for-mate ions,which were formed in situ in the solvothermal reaction.{[Zn(DIP)_(2)Cl]ClO_(4)}n(6)contains a layer structure formed by DIP and Zn^(2+).Free DPA and DIP ligands exhibited high fluorescence at room temperature,and coordina-tion polymers 3 and 6 displayed enhanced fluorescent emissions.
