目的研究扇贝裙边糖胺聚糖（glycosaminoglycan from Scallop Skirt，SS-GAG）体外抗单纯疱疹病毒Ⅰ型（HSV-Ⅰ）的作用。方法运用单纯疱疹病毒Ⅰ型（HSV-ⅠSM44株），并以非洲绿猴肾细胞（Vero细胞）为宿主细胞，通过观察病毒感染后的...目的研究扇贝裙边糖胺聚糖（glycosaminoglycan from Scallop Skirt，SS-GAG）体外抗单纯疱疹病毒Ⅰ型（HSV-Ⅰ）的作用。方法运用单纯疱疹病毒Ⅰ型（HSV-ⅠSM44株），并以非洲绿猴肾细胞（Vero细胞）为宿主细胞，通过观察病毒感染后的细胞变性反应（cytopathiceffect，CPE）和运用MTF比色法，检测不同浓度药物SS—GAG对Ⅰ型单纯疱疹病毒是否有直接的灭活作用、对HSV—Ⅰ感染复制的抑制活性以及对HSV—Ⅰ感染细胞的综合作用等，并观察药物对Vero细胞的毒性作用。结果与病毒对照组相比，SS-GAG各浓度组（100、50、25mg·L^-1）能有效地保护经HSV—Ⅰ感染的Vero细胞，使细胞活性增强（P〈0．01）；并减弱HSV—Ⅰ导致的病变效应，抑制病毒的复制。此作用随着药物浓度的增加而增强。但是SS—GAG对HSV—Ⅰ没有直接的灭活作用；SSGAG在50～1600mg·L^-1浓度范围内对Vero细胞无明显的细胞毒性。结论SS-GAG在体外实验系统中显示出明显的保护宿主细胞抵抗HSV-Ⅰ病毒感染的活性作用。展开更多
Aim:To study the antiatherosclerosis mechanism of scallop skirt glycosaminoglycan(SS-GAG)by observing the effect of SS-GAG on the oxidation of low density lipoprotein in macrophage and on the activities of glutathion ...Aim:To study the antiatherosclerosis mechanism of scallop skirt glycosaminoglycan(SS-GAG)by observing the effect of SS-GAG on the oxidation of low density lipoprotein in macrophage and on the activities of glutathion peroxidase(GSH-PX)and myeloperoxidase(MPO).Methods:The cell line was established by in vitro culturing mouse macrophage RAW264.7 was employed to study the effects of SS-GAG on the oxidation of LDL and the activities of relevant enzymes in macrophage via the assay of concentrations of malonaldehyde(MDA),conjugated diene,activity of GSH-PX,and MPO.Results:After macrophage incubation with LDL for 24 h,we found the enhanced activity of MPO,decreased activity of GSH-PX,and increased production of lipid peroxidation.Moreover,SS-GAG could increase the activity of GSH-PX but attenuate the activity of MPO.The decreased production of lipid peroxidation were also observed.There also existed dose-effect relationship in our findings.Conclusion:SS-GAG could depress the oxidation of LDL by degrading the activity of MPO and increasing the activity of GSH-PX.This may be one of its mechanisms of antiatherosclerosis action.展开更多
文摘目的研究扇贝裙边糖胺聚糖（glycosaminoglycan from Scallop Skirt，SS-GAG）体外抗单纯疱疹病毒Ⅰ型（HSV-Ⅰ）的作用。方法运用单纯疱疹病毒Ⅰ型（HSV-ⅠSM44株），并以非洲绿猴肾细胞（Vero细胞）为宿主细胞，通过观察病毒感染后的细胞变性反应（cytopathiceffect，CPE）和运用MTF比色法，检测不同浓度药物SS—GAG对Ⅰ型单纯疱疹病毒是否有直接的灭活作用、对HSV—Ⅰ感染复制的抑制活性以及对HSV—Ⅰ感染细胞的综合作用等，并观察药物对Vero细胞的毒性作用。结果与病毒对照组相比，SS-GAG各浓度组（100、50、25mg·L^-1）能有效地保护经HSV—Ⅰ感染的Vero细胞，使细胞活性增强（P〈0．01）；并减弱HSV—Ⅰ导致的病变效应，抑制病毒的复制。此作用随着药物浓度的增加而增强。但是SS—GAG对HSV—Ⅰ没有直接的灭活作用；SSGAG在50～1600mg·L^-1浓度范围内对Vero细胞无明显的细胞毒性。结论SS-GAG在体外实验系统中显示出明显的保护宿主细胞抵抗HSV-Ⅰ病毒感染的活性作用。
文摘Aim:To study the antiatherosclerosis mechanism of scallop skirt glycosaminoglycan(SS-GAG)by observing the effect of SS-GAG on the oxidation of low density lipoprotein in macrophage and on the activities of glutathion peroxidase(GSH-PX)and myeloperoxidase(MPO).Methods:The cell line was established by in vitro culturing mouse macrophage RAW264.7 was employed to study the effects of SS-GAG on the oxidation of LDL and the activities of relevant enzymes in macrophage via the assay of concentrations of malonaldehyde(MDA),conjugated diene,activity of GSH-PX,and MPO.Results:After macrophage incubation with LDL for 24 h,we found the enhanced activity of MPO,decreased activity of GSH-PX,and increased production of lipid peroxidation.Moreover,SS-GAG could increase the activity of GSH-PX but attenuate the activity of MPO.The decreased production of lipid peroxidation were also observed.There also existed dose-effect relationship in our findings.Conclusion:SS-GAG could depress the oxidation of LDL by degrading the activity of MPO and increasing the activity of GSH-PX.This may be one of its mechanisms of antiatherosclerosis action.
