Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus...Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus caracteristicus collected from South China Sea was studied in this work.A new conotoxin,cr5a,was isolated from the venom through Sephadex G-25 chromatography and HPLC,and then was identified by（amino） acid analysis,mass spectrometry,N-terminal sequence.The primary structure of cr5a was CCKFQFLNFCCNE,it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues（CC-CC）.Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved,and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys1-Cys10, Cys2-Cys11.So cr5a is a new member of the T-superfamily conotoxins.展开更多
文摘本文利用笼形超分子材料穴番-A对甲烷分子的特异性包合作用,提出了一种能够在室温下工作的采用穴番-A敏感膜的新型声表面波瓦斯传感器。设计研制了中心频率为300 MHz的低损耗、高Q值SAW谐振器,并以所研制的谐振器构成双通道差分振荡器。分别以点涂法和旋涂法在传感通道SAW谐振器表面进行了敏感膜的镀膜。通过实验观察对比了采用两种镀膜方式的SAW瓦斯传感器在常温下对5%甲烷气体的响应。实验结果显示点涂镀膜的传感器响应约为1 k Hz,远大于旋涂镀膜方式。AFM表面形貌表征显示了敏感膜表面粗糙度是造成这一差异的主要原因。实验还研究了传感器对不同浓度甲烷气体的响应,结果表明传感器响应随气体浓度降低而减小,两者存在良好的线性关系,测试灵敏度为205 Hz/%,检测下限约为0.2%。
文摘Cone snails are tropical marine predatory molluscs,and there is an unprecedented variety of neuropharmacologically active peptide.Over seventy living species of cone snails are distributed in China.The ve-nom of Conus caracteristicus collected from South China Sea was studied in this work.A new conotoxin,cr5a,was isolated from the venom through Sephadex G-25 chromatography and HPLC,and then was identified by（amino） acid analysis,mass spectrometry,N-terminal sequence.The primary structure of cr5a was CCKFQFLNFCCNE,it shared a conserved T-superfamily of conotoxins arrangement of cysteine residues（CC-CC）.Total chemical synthesis of the two possible disulfide linkages of cr5a was achieved,and the result of HPLC co-elution showed that cr5a was a tridecapeptide with a disulfide connectivity of Cys1-Cys10, Cys2-Cys11.So cr5a is a new member of the T-superfamily conotoxins.
