The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially exp...The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially expressed genes between K562 cells and the matrine treated cells. The sequences of the fragments of the differentially expressed genes were obtained by sequencing following the T A cloned fragments identified by restriction endonuclease, and analyzed with bioinformatics. The results indicated that the multiple gene expression known already changed in the process of induced cell differentiation and an unknown gene fragment was cloned. It suggested that the differentially expressed genes acted as cell differentiation related genes involved in the early phase of the induced cell differentiation.展开更多
妊娠相关血浆蛋白A(Pregnancy-as-sociated plasma protein A,PAPPA)是存在于孕血中的一种大分子糖蛋白,1974年由Lin等首次报道并从孕血中分离纯化。该蛋白质也有其它名称:妊娠特异性a_2迁移蛋白、人精浆免疫抑制物、男性抑制物等,但以P...妊娠相关血浆蛋白A(Pregnancy-as-sociated plasma protein A,PAPPA)是存在于孕血中的一种大分子糖蛋白,1974年由Lin等首次报道并从孕血中分离纯化。该蛋白质也有其它名称:妊娠特异性a_2迁移蛋白、人精浆免疫抑制物、男性抑制物等,但以PAPPA最常用。迄今,人们对PAP-PA的理化性质、合成部位、生理功能以及临床意义等方面的研究已有一定进展,分别综述如下。展开更多
文摘The differentiation of human leukemia K562 cells can be induced by matrine. To investigate the mechanism of the induced cell differentiation, an improved DDRT PCR technique was applied to screen the differentially expressed genes between K562 cells and the matrine treated cells. The sequences of the fragments of the differentially expressed genes were obtained by sequencing following the T A cloned fragments identified by restriction endonuclease, and analyzed with bioinformatics. The results indicated that the multiple gene expression known already changed in the process of induced cell differentiation and an unknown gene fragment was cloned. It suggested that the differentially expressed genes acted as cell differentiation related genes involved in the early phase of the induced cell differentiation.
文摘妊娠相关血浆蛋白A(Pregnancy-as-sociated plasma protein A,PAPPA)是存在于孕血中的一种大分子糖蛋白,1974年由Lin等首次报道并从孕血中分离纯化。该蛋白质也有其它名称:妊娠特异性a_2迁移蛋白、人精浆免疫抑制物、男性抑制物等,但以PAPPA最常用。迄今,人们对PAP-PA的理化性质、合成部位、生理功能以及临床意义等方面的研究已有一定进展,分别综述如下。
文摘本文采用硫酸铵盐析、DE-52离子交换层析、Sephadex G-200凝胶过滤及抗SP_1免疫亲和柱层析等方法从胎盘后血分离妊娠特异性β_1民糖蛋白(SP_1)所得SP_1纯化约207倍.纯化的SP_1与德国Behringwerke AG的SP_1抗血清在双向免疫扩散时有沉淀反应;免疫电泳时纯化SP_1移动的位置与β蛋白相当.聚丙烯酰胺凝胶电泳时纯化的SP_1呈现一条主带其分子量为90,000左右.免疫家免所得的抗血清经血浆蛋白吸收后得特异SP_1抗血清在双向免疫扩散时与正常男子或非孕妇女血清均无沉淀反应;与孕血反应形成的沉淀线与德国Behring werke AG的SP_1抗血清所形成的沉淀线相连;交义免疫电泳时呈现一个峰.
