Sixteen o alkoxycarbonylphenyl sulfonamide derivatives were synthesized.Their structures were confirmed by elemental analysis and 1HNMR,some of which being identified by IR and MS.The preliminary biological tests show...Sixteen o alkoxycarbonylphenyl sulfonamide derivatives were synthesized.Their structures were confirmed by elemental analysis and 1HNMR,some of which being identified by IR and MS.The preliminary biological tests showed that some of them exhibited a degree of herbicidal and plant growth regulation activity.展开更多
目的:制备抗人内皮抑素的单克隆抗体(mAb),为深入研究内皮抑素的作用机制提供一种有用的试剂。方法:以毕赤酵母表达的内皮抑索为免疫原免疫BALB/c小鼠,采用B细胞杂交瘤技术,以间接ELISA法筛选分泌抗人内皮抑素的特异性mAb的杂交瘤细胞株...目的:制备抗人内皮抑素的单克隆抗体(mAb),为深入研究内皮抑素的作用机制提供一种有用的试剂。方法:以毕赤酵母表达的内皮抑索为免疫原免疫BALB/c小鼠,采用B细胞杂交瘤技术,以间接ELISA法筛选分泌抗人内皮抑素的特异性mAb的杂交瘤细胞株,并诱生腹水。腹水中的mAb采用 Protein A Sepharose CL-4B进行纯化。以标准的抗Ig血清做ELISA,鉴定mAb的Ig类、亚类及型;用免疫印迹法鉴定mAb的特异性。结果:获得1株可分泌抗人内皮抑素mAb的杂交瘤细胞株4E7,该株杂交细胞分泌的mAb属于IgGl(λ型)。腹水mAb的效价为1×10-4-1×10-6,纯化后大于1×10-10。免疫印迹结果显示,mAb 4E7可特异性地与酵母及大肠杆菌表达的内皮抑素起反应,而与bFGF等其他细胞因子不发生交叉反应。结论:分泌抗人内皮抑素mAb杂交瘤细胞株的建立,为进一步研究奠定了基础。展开更多
文摘Sixteen o alkoxycarbonylphenyl sulfonamide derivatives were synthesized.Their structures were confirmed by elemental analysis and 1HNMR,some of which being identified by IR and MS.The preliminary biological tests showed that some of them exhibited a degree of herbicidal and plant growth regulation activity.
文摘目的:制备抗人内皮抑素的单克隆抗体(mAb),为深入研究内皮抑素的作用机制提供一种有用的试剂。方法:以毕赤酵母表达的内皮抑索为免疫原免疫BALB/c小鼠,采用B细胞杂交瘤技术,以间接ELISA法筛选分泌抗人内皮抑素的特异性mAb的杂交瘤细胞株,并诱生腹水。腹水中的mAb采用 Protein A Sepharose CL-4B进行纯化。以标准的抗Ig血清做ELISA,鉴定mAb的Ig类、亚类及型;用免疫印迹法鉴定mAb的特异性。结果:获得1株可分泌抗人内皮抑素mAb的杂交瘤细胞株4E7,该株杂交细胞分泌的mAb属于IgGl(λ型)。腹水mAb的效价为1×10-4-1×10-6,纯化后大于1×10-10。免疫印迹结果显示,mAb 4E7可特异性地与酵母及大肠杆菌表达的内皮抑素起反应,而与bFGF等其他细胞因子不发生交叉反应。结论:分泌抗人内皮抑素mAb杂交瘤细胞株的建立,为进一步研究奠定了基础。
