A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(...A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1 0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.展开更多
文摘A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1 0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.
