To study effects of erythrocyte on T cell depletion by immutoxin (IT). MTT assays,trypan blue dyeexclusion assays, colony-forming assays were used to measure lymphocytestransformation capacity, recovery of nucleated c...To study effects of erythrocyte on T cell depletion by immutoxin (IT). MTT assays,trypan blue dyeexclusion assays, colony-forming assays were used to measure lymphocytestransformation capacity, recovery of nucleated cell, cell viability and the growth ofhematopoietic progenitor in the cord blood without separated erythrocytes and the cord bloodwith separated erythrocytes (the mononuclear cell group) before and after T cell depletion byCD5+:Ricin at the dosage of 10-9mmol/L. The results showed that lymphocytes transforma-tion capacity were no significant difference among three groups; the recovery of nucleatedcell, the viability of cell and all of the proliferative rate of hematopoietic progenitor weregreater than 90% ,90% and 70%, respectivity. Lymphocytes transformation capacity waseffectivety inhibited after CD5+: Ricin treated the cord blood without separated erythocytes.In conclusion, there is no effect of erythrocyte on the T cell depletion.展开更多
文摘To study effects of erythrocyte on T cell depletion by immutoxin (IT). MTT assays,trypan blue dyeexclusion assays, colony-forming assays were used to measure lymphocytestransformation capacity, recovery of nucleated cell, cell viability and the growth ofhematopoietic progenitor in the cord blood without separated erythrocytes and the cord bloodwith separated erythrocytes (the mononuclear cell group) before and after T cell depletion byCD5+:Ricin at the dosage of 10-9mmol/L. The results showed that lymphocytes transforma-tion capacity were no significant difference among three groups; the recovery of nucleatedcell, the viability of cell and all of the proliferative rate of hematopoietic progenitor weregreater than 90% ,90% and 70%, respectivity. Lymphocytes transformation capacity waseffectivety inhibited after CD5+: Ricin treated the cord blood without separated erythocytes.In conclusion, there is no effect of erythrocyte on the T cell depletion.
