A simple and rapid capillary electrophoresis(CE) method is firstly established for the separation and determination of rutin and quercetin in Gansu particle.After a series of experiments,10 mmol·L-1 borate buffer...A simple and rapid capillary electrophoresis(CE) method is firstly established for the separation and determination of rutin and quercetin in Gansu particle.After a series of experiments,10 mmol·L-1 borate buffer containing 5% acetonitrile at pH 9.4 is selected as the optimum,the separation voltage and temperature are 20 kV and 25℃ respectively.In the tested concentration range,good linear relationships between the peak area and the concentration of the analytes are revealed(correlation coefficients:Rutin 0.9996 and Quercetin 0.9995).Finally,the method is successfully applied to the analysis of the two compounds in Gansu Particle sample.展开更多
文摘A simple and rapid capillary electrophoresis(CE) method is firstly established for the separation and determination of rutin and quercetin in Gansu particle.After a series of experiments,10 mmol·L-1 borate buffer containing 5% acetonitrile at pH 9.4 is selected as the optimum,the separation voltage and temperature are 20 kV and 25℃ respectively.In the tested concentration range,good linear relationships between the peak area and the concentration of the analytes are revealed(correlation coefficients:Rutin 0.9996 and Quercetin 0.9995).Finally,the method is successfully applied to the analysis of the two compounds in Gansu Particle sample.
