为了从猪血中分离纯化高纯度的猪血红蛋白,建立了通过超滤、DEAE-Sepharose Fast Flow离子交换色谱和Sephadex G-75凝胶排阻色谱三步法制备高纯度猪血红蛋白的方法,并通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效凝胶排阻色...为了从猪血中分离纯化高纯度的猪血红蛋白,建立了通过超滤、DEAE-Sepharose Fast Flow离子交换色谱和Sephadex G-75凝胶排阻色谱三步法制备高纯度猪血红蛋白的方法,并通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效凝胶排阻色谱和反相高效液相色谱方法,对纯化后的猪血红蛋白进行了鉴定。经三步分离纯化后,猪血红蛋白的纯度大于99%,含量为1.328g/L。展开更多
A mathematical model that described the strains growth, product formation and medium consumption was presented to predict the performance of batch fermentation of Zoogloea sp.GY3 which was screened from activated slud...A mathematical model that described the strains growth, product formation and medium consumption was presented to predict the performance of batch fermentation of Zoogloea sp.GY3 which was screened from activated sludge and could synthesize PHAs.A simple model was proposed by using the Logistic equation for growth, the Luedeking-Piret equation for PHAs production and the Luedeking-Piret-like equation for sucrose consumption.The model appeared to provide a reasonable description for each parameter during the growth phase.The production of PHAs was growth-associated.The models were in good agreement with the measured data in experiments.展开更多
文摘为了从猪血中分离纯化高纯度的猪血红蛋白,建立了通过超滤、DEAE-Sepharose Fast Flow离子交换色谱和Sephadex G-75凝胶排阻色谱三步法制备高纯度猪血红蛋白的方法,并通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效凝胶排阻色谱和反相高效液相色谱方法,对纯化后的猪血红蛋白进行了鉴定。经三步分离纯化后,猪血红蛋白的纯度大于99%,含量为1.328g/L。
文摘A mathematical model that described the strains growth, product formation and medium consumption was presented to predict the performance of batch fermentation of Zoogloea sp.GY3 which was screened from activated sludge and could synthesize PHAs.A simple model was proposed by using the Logistic equation for growth, the Luedeking-Piret equation for PHAs production and the Luedeking-Piret-like equation for sucrose consumption.The model appeared to provide a reasonable description for each parameter during the growth phase.The production of PHAs was growth-associated.The models were in good agreement with the measured data in experiments.
