Objective:To study the immune response of CD8+T cell from HLA-A2+ patients with Nasoparygeal cancer(NPC), induced by autologous dendritic cells(DC) loaded with HLA-A2-restricted epitope peptides from EBV latent membra...Objective:To study the immune response of CD8+T cell from HLA-A2+ patients with Nasoparygeal cancer(NPC), induced by autologous dendritic cells(DC) loaded with HLA-A2-restricted epitope peptides from EBV latent membrane protein 2(LMP-2).Methods:Get DCs by culturing plastic-adherent monocytes isolated from NPC patients of HLA-A2+ peripheral blood with cytokine. Stimulating autologous CD8+T with DCs pulsed with the peptides CLGGLLTMV(CLG) and LTAGFIFL(LTA), the HLA-A2-restricted epitope peptides from LMP-2 protein. After 2 weeks for culture, frequencies of CD8+T cells to secret IFN-garmma in response to peptides were detected by Elispot assay and peptide-specific CD8+T cells were measured by tetramer staining.Results:The frequencies of effector cells to secrete IFN-gamma in response to CLG and LTA before DC presentation were (2.67±1.97)/well and (5.33 ±1.86)/well, respectively. After DC stimulation, the number was (42.67±33.79)/well and (25.67±18.25)/well respectively. They all were increased significantly after DC presentation(P<0.05). The median frequencies of CLG-specific CD8+T cell and LTA-specific CD8+T cell were 0.135% and 1.140% respectively before DC presentation; after presenting they were 1.045% and 1.945% respectively. The frequencies of the two specific CD8+T cells were enhanced significantly after DC stimulation(P<0.05).Conclusion:The DC loaded with the epitope peptides of LMP-2 from EBV can induce CD8+T cells to cytotoxic T lymphocytes(CTLs), with clinical potential for immunotherapy.展开更多
文摘Objective:To study the immune response of CD8+T cell from HLA-A2+ patients with Nasoparygeal cancer(NPC), induced by autologous dendritic cells(DC) loaded with HLA-A2-restricted epitope peptides from EBV latent membrane protein 2(LMP-2).Methods:Get DCs by culturing plastic-adherent monocytes isolated from NPC patients of HLA-A2+ peripheral blood with cytokine. Stimulating autologous CD8+T with DCs pulsed with the peptides CLGGLLTMV(CLG) and LTAGFIFL(LTA), the HLA-A2-restricted epitope peptides from LMP-2 protein. After 2 weeks for culture, frequencies of CD8+T cells to secret IFN-garmma in response to peptides were detected by Elispot assay and peptide-specific CD8+T cells were measured by tetramer staining.Results:The frequencies of effector cells to secrete IFN-gamma in response to CLG and LTA before DC presentation were (2.67±1.97)/well and (5.33 ±1.86)/well, respectively. After DC stimulation, the number was (42.67±33.79)/well and (25.67±18.25)/well respectively. They all were increased significantly after DC presentation(P<0.05). The median frequencies of CLG-specific CD8+T cell and LTA-specific CD8+T cell were 0.135% and 1.140% respectively before DC presentation; after presenting they were 1.045% and 1.945% respectively. The frequencies of the two specific CD8+T cells were enhanced significantly after DC stimulation(P<0.05).Conclusion:The DC loaded with the epitope peptides of LMP-2 from EBV can induce CD8+T cells to cytotoxic T lymphocytes(CTLs), with clinical potential for immunotherapy.
