The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and c...The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in vivo . FRET is an ideal technology for detection of protein conformation and protein-protein interaction by using fluorescence protein, traditional organic dyes and other dyes as probes. It uses fluorescence protein, traditional organic dyes and other dyes as its probes. The application of FRET in the determination of intracellular events would be helpful for us to understand the structure and function of biology molecules. [展开更多
NF-κB is thought of as a genetic switch to control expressions of many target genes and directly participates in pathogenesis of infection, inflammation, stress, immunoresponse, cellular apoptosis, toxic shock and tu...NF-κB is thought of as a genetic switch to control expressions of many target genes and directly participates in pathogenesis of infection, inflammation, stress, immunoresponse, cellular apoptosis, toxic shock and tumor as well as cell-cycle regulation and cell differentiation. The overactivation of NF-κB is intimately involved in many human diseases. Various therapeutic strategies against NF-κB, to date, include anti-inflammatory drugs, antioxidants, immunosuppressive agents, inhibitors of protease and proteasome, prostaglandings, nitric oxide, IL-10, microbial products, synthetic inhibitors, antisense oligonucleotides and decoy deoxyoligonucleotides. Studies are underway to develop NF-κB member-specific and cell type-specific drugs that can inhibit the activation of NF-κB only in target cells and that may become a novel way to treat the human diseases.展开更多
文摘The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in vivo . FRET is an ideal technology for detection of protein conformation and protein-protein interaction by using fluorescence protein, traditional organic dyes and other dyes as probes. It uses fluorescence protein, traditional organic dyes and other dyes as its probes. The application of FRET in the determination of intracellular events would be helpful for us to understand the structure and function of biology molecules. [
文摘NF-κB is thought of as a genetic switch to control expressions of many target genes and directly participates in pathogenesis of infection, inflammation, stress, immunoresponse, cellular apoptosis, toxic shock and tumor as well as cell-cycle regulation and cell differentiation. The overactivation of NF-κB is intimately involved in many human diseases. Various therapeutic strategies against NF-κB, to date, include anti-inflammatory drugs, antioxidants, immunosuppressive agents, inhibitors of protease and proteasome, prostaglandings, nitric oxide, IL-10, microbial products, synthetic inhibitors, antisense oligonucleotides and decoy deoxyoligonucleotides. Studies are underway to develop NF-κB member-specific and cell type-specific drugs that can inhibit the activation of NF-κB only in target cells and that may become a novel way to treat the human diseases.
