Objective:To anatomically and phenotypically characterize the insular cortex(IC)-nucleus tractus soli-tari(NTS)neural pathway.Methods:Adult male Sprague-Dawley rats were divided into three experimental cohorts for neu...Objective:To anatomically and phenotypically characterize the insular cortex(IC)-nucleus tractus soli-tari(NTS)neural pathway.Methods:Adult male Sprague-Dawley rats were divided into three experimental cohorts for neural circuit tracing.Anterograde labeling was achieved by injecting anterograde self-complementary adeno-associated viruses(scAAVs)into the IC.Retrograde tracing involved NTS injections of either retrograde scAAVs or FluoroGold(FG),combined with immunofluorescence histochemical staining to identify IC-originating projection neurons.For postsynaptic neurochemical phenotype characterization,IC was injected with AAV2/1-CaMKII-Cre,while a mixture of AAV2/9-Syn-DIO-mCherry and AAV2/9-VGAT1-EGFP was injected into the NTS.The rats were allowed to survive for one week following scAAVs or FG injection or four weeks after recombinase-dependent systems injection.Then the rats were sacrificed,and serial brain sections were prepared for immunofluorescence histochemical staining(brain section containing FG)and subsequent fluorescence/confocal microscopic analysis.Results:(1)Anterograde viral tracing re-vealed dense axonal terminals from the IC projecting to the medial subnucleus of the NTS,while retrograde tracing re-vealed that IC neurons projecting to the NTS were predominantly localized within the dysgranular layer;(2)IC-NTS projection neurons were exclusive glutamatergic(100%,n=3);(3)NTS neurons receiving IC inputs were mainly lo-calized in the medial subnucleus,and were predominantly GABAergic(79.8±3.2%,n=3).Conclusion:The pres-ent results indicate that a descending pathway from excitatory neurons of the IC terminates onto inhibitory neurons of the NTS,which might represent a potential neuromodulatory target for visceral pain disorders.展开更多
由空军军医大学人体解剖与组织胚胎学教研室李云庆教授、空军军医大学西京医院神经内科赵刚教授、复旦大学中山医院神经内科汪昕教授和大连医科大学人体解剖教研室隋鸿锦教授主译的《临床神经解剖学:病例解析》(Neuroanatomy through Cl...由空军军医大学人体解剖与组织胚胎学教研室李云庆教授、空军军医大学西京医院神经内科赵刚教授、复旦大学中山医院神经内科汪昕教授和大连医科大学人体解剖教研室隋鸿锦教授主译的《临床神经解剖学:病例解析》(Neuroanatomy through Clinical Cases,第2版)已于2021年3月由天津出版传媒集团出版社出版发行。展开更多
目的:观察脊髓背角P物质受体(substance P receptor,SPR)阳性神经元向臂旁核及中线/板内核群的分支投射并为其参与痒觉信息传递提供证据。方法:利用小动物脑立体定位仪向10只8周龄C57BL/6小鼠的臂旁核及丘脑中线/板内核群内分别注射四...目的:观察脊髓背角P物质受体(substance P receptor,SPR)阳性神经元向臂旁核及中线/板内核群的分支投射并为其参与痒觉信息传递提供证据。方法:利用小动物脑立体定位仪向10只8周龄C57BL/6小鼠的臂旁核及丘脑中线/板内核群内分别注射四甲基罗达明(tetramethylrhodamine-dextran,TMR)及荧光金(fluoro-gold,FG),观察小鼠脊髓背角的TMR/FG双标神经元。结合免疫荧光组织化学染色方法观察双标神经元表达SPR和在急性痒模型下表达Fos的情况。结果:脊髓背角I层、脊髓外侧核(lateral spinal nucleus,LSN)及脊髓背角IV～V层均可观察到TMR/FG双标神经元,且部分双标神经元分别表达SPR及Fos蛋白。结论:脊髓背角存在同时向臂旁核及丘脑中线/板内核群发出分支投射的神经元,部分双标神经元表达SPR并参与痒觉信息的传递。展开更多
由中国解剖学会副理事长、国际解剖学工作者联盟(IFAA)会议主席、国际形态学大会(ISMS)和亚太地区解剖学会(APICA)执行委员、《神经解剖学杂志》主编、空军军医大学(原第四军医大学)人体解剖与组织胚胎学教研室李云庆教授主译(史娟老师...由中国解剖学会副理事长、国际解剖学工作者联盟(IFAA)会议主席、国际形态学大会(ISMS)和亚太地区解剖学会(APICA)执行委员、《神经解剖学杂志》主编、空军军医大学(原第四军医大学)人体解剖与组织胚胎学教研室李云庆教授主译(史娟老师和陈晶老师副主译)的《人脑图谱》(Atlas of the Human Brain,第4版)已于2020年7月由河南科学技术出版社出版发行。展开更多
由中国解剖学会副理事长、国际解剖学工作者联盟(IFAA)会议主席、国际形态学大会(ISMS)和亚太地区解剖学会(APICA)执行委员、《神经解剖学杂志》主编、空军军医大学(原第四军医大学)人体解剖与组织胚胎学教研室李云庆教授主译(史娟老师...由中国解剖学会副理事长、国际解剖学工作者联盟(IFAA)会议主席、国际形态学大会(ISMS)和亚太地区解剖学会(APICA)执行委员、《神经解剖学杂志》主编、空军军医大学(原第四军医大学)人体解剖与组织胚胎学教研室李云庆教授主译(史娟老师和陈晶老师副主译)的《人脑图谱》(Atlas of the Human Brain,第4版)已于2020年7月由河南科学技术出版社出版发行。展开更多
文摘Objective:To anatomically and phenotypically characterize the insular cortex(IC)-nucleus tractus soli-tari(NTS)neural pathway.Methods:Adult male Sprague-Dawley rats were divided into three experimental cohorts for neural circuit tracing.Anterograde labeling was achieved by injecting anterograde self-complementary adeno-associated viruses(scAAVs)into the IC.Retrograde tracing involved NTS injections of either retrograde scAAVs or FluoroGold(FG),combined with immunofluorescence histochemical staining to identify IC-originating projection neurons.For postsynaptic neurochemical phenotype characterization,IC was injected with AAV2/1-CaMKII-Cre,while a mixture of AAV2/9-Syn-DIO-mCherry and AAV2/9-VGAT1-EGFP was injected into the NTS.The rats were allowed to survive for one week following scAAVs or FG injection or four weeks after recombinase-dependent systems injection.Then the rats were sacrificed,and serial brain sections were prepared for immunofluorescence histochemical staining(brain section containing FG)and subsequent fluorescence/confocal microscopic analysis.Results:(1)Anterograde viral tracing re-vealed dense axonal terminals from the IC projecting to the medial subnucleus of the NTS,while retrograde tracing re-vealed that IC neurons projecting to the NTS were predominantly localized within the dysgranular layer;(2)IC-NTS projection neurons were exclusive glutamatergic(100%,n=3);(3)NTS neurons receiving IC inputs were mainly lo-calized in the medial subnucleus,and were predominantly GABAergic(79.8±3.2%,n=3).Conclusion:The pres-ent results indicate that a descending pathway from excitatory neurons of the IC terminates onto inhibitory neurons of the NTS,which might represent a potential neuromodulatory target for visceral pain disorders.
文摘由空军军医大学人体解剖与组织胚胎学教研室李云庆教授、空军军医大学西京医院神经内科赵刚教授、复旦大学中山医院神经内科汪昕教授和大连医科大学人体解剖教研室隋鸿锦教授主译的《临床神经解剖学:病例解析》(Neuroanatomy through Clinical Cases,第2版)已于2021年3月由天津出版传媒集团出版社出版发行。
文摘目的:观察脊髓背角P物质受体(substance P receptor,SPR)阳性神经元向臂旁核及中线/板内核群的分支投射并为其参与痒觉信息传递提供证据。方法:利用小动物脑立体定位仪向10只8周龄C57BL/6小鼠的臂旁核及丘脑中线/板内核群内分别注射四甲基罗达明(tetramethylrhodamine-dextran,TMR)及荧光金(fluoro-gold,FG),观察小鼠脊髓背角的TMR/FG双标神经元。结合免疫荧光组织化学染色方法观察双标神经元表达SPR和在急性痒模型下表达Fos的情况。结果:脊髓背角I层、脊髓外侧核(lateral spinal nucleus,LSN)及脊髓背角IV～V层均可观察到TMR/FG双标神经元,且部分双标神经元分别表达SPR及Fos蛋白。结论:脊髓背角存在同时向臂旁核及丘脑中线/板内核群发出分支投射的神经元,部分双标神经元表达SPR并参与痒觉信息的传递。
文摘由中国解剖学会副理事长、国际解剖学工作者联盟(IFAA)会议主席、国际形态学大会(ISMS)和亚太地区解剖学会(APICA)执行委员、《神经解剖学杂志》主编、空军军医大学(原第四军医大学)人体解剖与组织胚胎学教研室李云庆教授主译(史娟老师和陈晶老师副主译)的《人脑图谱》(Atlas of the Human Brain,第4版)已于2020年7月由河南科学技术出版社出版发行。
文摘由中国解剖学会副理事长、国际解剖学工作者联盟(IFAA)会议主席、国际形态学大会(ISMS)和亚太地区解剖学会(APICA)执行委员、《神经解剖学杂志》主编、空军军医大学(原第四军医大学)人体解剖与组织胚胎学教研室李云庆教授主译(史娟老师和陈晶老师副主译)的《人脑图谱》(Atlas of the Human Brain,第4版)已于2020年7月由河南科学技术出版社出版发行。
