DNA extracted directly from the living nodules of Casuarina cunninghamiana, C.collina, C.glauca, Alnus cremastogyne, A.trabeculosa and Myrica rubra and also from 21 Frankia strains isolated from the root nodules of th...DNA extracted directly from the living nodules of Casuarina cunninghamiana, C.collina, C.glauca, Alnus cremastogyne, A.trabeculosa and Myrica rubra and also from 21 Frankia strains isolated from the root nodules of the actinorhizal plants in Fujian, including C. cunninghamiana, C.equisetifolia, C.glauca, A.cremastogyne and M.rubra. PCR amplification was conducted with the primers targeting the 3’ end of the 16S rDNA, the IGS, and the 5’ part of the 23S rDNA (i.e.,rrn region). PCR products were then analyzed by using a set of restriction endonucleases. Two distinct genetic groups were recognized on the basis of these restriction patterns. All Frankia strains associated with the host species of Casuarina were assigned to the same group. Frankia living in the nodules of Myrica and Alnus belonged to the other group. In Myrica-Alnus group, there was two sub-group which one included A.cremastogyme and the other contained A.trabeculosa and M.rubra. The results of RFLP analysis showed that the genetic diversity of Frankia associated with Casuarina could be lower, but Frankia existed in the soils of Fujian Province would have more richness in genetic diversity. The results also reflected that host plant has an ability to choose the strains to form a symbiont.展开更多
To provide potential implications of species selection for carbon plantation, differences in carbon sequestration pattern and net ecosystem production (NEP) were determined between two 36-year-old plantations of broad...To provide potential implications of species selection for carbon plantation, differences in carbon sequestration pattern and net ecosystem production (NEP) were determined between two 36-year-old plantations of broadleaved species, Castanopsis kawakamii and Cunninghamia lanceolata, in Sanming, Fujian. Annual net carbon sequestration was 13.639 and 6.599 t C·hm -2 a -1 , respectively, in the C. kawakamii and the C. lanceolata, among which the annual biomass C increment and the litterfall production was evenly distributed. To the annual net carbon sequestration, the contribution of stem (wood plus bark) increment was much lower, and that of branch increment was much higher in the C. kawakamii than in the C. lanceolata (26.6% versus 40.3%, and 11.5% versus 0.3%). In both plantation, the above and belowground litterfall accounted 60% and 40% respectively for the annual litterfall production, which was estimated 7.183 t C·hm -2 a -1 in the C. kawakamii and 3.554 t C·hm -2 a -1 in the C. lanceolata. Annual soil heterotrophic respiration was responsible for a C loss of 5.983 and 2.984 t·hm -2 a -1 from soil to atmosphere in the C. kawakamii and the C. lanceolata, respectively. Carbon balance analysis showed there were a positive net ecosystem production (C sink), 7.656 and 3.615 t C·hm -2 a -1 , for the C. kawakamii and the C. lanceolata, respectively. For the purpose of carbon management, C. kawakamii might be a more suitable species than C. lanceolata in local region.展开更多
为探讨软腐病菌侵染下菜心转录组功能基因信息,采用BGISEQ-500高通量测序技术对软腐病菌侵染下菜心叶片进行转录组测序,平均每个样获得47.27 M clean reads,Q20值均大于95%,共检测到表达的Unigene为36760个,其中已知的有35327个,预测新U...为探讨软腐病菌侵染下菜心转录组功能基因信息,采用BGISEQ-500高通量测序技术对软腐病菌侵染下菜心叶片进行转录组测序,平均每个样获得47.27 M clean reads,Q20值均大于95%,共检测到表达的Unigene为36760个,其中已知的有35327个,预测新Unigene有1433个;共检测出19549个新转录本,其长度主要集中在300~2000 nt。功能注释结果显示,有32047个Unigene在Nr数据库获得注释,其中注释到芸薹属白菜上的Unigene最多;GO功能共注释到12588个Unigene;KEGG数据库注释到18583个Unigene,涉及到137个代谢通路。共获得21776个组间差异表达基因(differential expression genes,DEGs),其中对照与发病前期组间的DEGs有15007个,6137个上调表达,8870个下调表达;发病前期与发病中期组间DEGs有13118个,10278个上调表达,2840个下调表达;发病中期与发病后期组间DEGs有11293个,1790个上调表达,9503个下调表达。DEGs的Ven图分析显示5110个基因在每组间均存在差异,DEGs功能分析显示DEGs参与泛素蛋白降解途径、过氧化物酶体途径、光合碳固定途径、糖酵解途径等多种生命活动。本研究结果为深入开展菜心抗软腐病基因组学和分子生物学研究奠定基础。展开更多
文摘DNA extracted directly from the living nodules of Casuarina cunninghamiana, C.collina, C.glauca, Alnus cremastogyne, A.trabeculosa and Myrica rubra and also from 21 Frankia strains isolated from the root nodules of the actinorhizal plants in Fujian, including C. cunninghamiana, C.equisetifolia, C.glauca, A.cremastogyne and M.rubra. PCR amplification was conducted with the primers targeting the 3’ end of the 16S rDNA, the IGS, and the 5’ part of the 23S rDNA (i.e.,rrn region). PCR products were then analyzed by using a set of restriction endonucleases. Two distinct genetic groups were recognized on the basis of these restriction patterns. All Frankia strains associated with the host species of Casuarina were assigned to the same group. Frankia living in the nodules of Myrica and Alnus belonged to the other group. In Myrica-Alnus group, there was two sub-group which one included A.cremastogyme and the other contained A.trabeculosa and M.rubra. The results of RFLP analysis showed that the genetic diversity of Frankia associated with Casuarina could be lower, but Frankia existed in the soils of Fujian Province would have more richness in genetic diversity. The results also reflected that host plant has an ability to choose the strains to form a symbiont.
文摘To provide potential implications of species selection for carbon plantation, differences in carbon sequestration pattern and net ecosystem production (NEP) were determined between two 36-year-old plantations of broadleaved species, Castanopsis kawakamii and Cunninghamia lanceolata, in Sanming, Fujian. Annual net carbon sequestration was 13.639 and 6.599 t C·hm -2 a -1 , respectively, in the C. kawakamii and the C. lanceolata, among which the annual biomass C increment and the litterfall production was evenly distributed. To the annual net carbon sequestration, the contribution of stem (wood plus bark) increment was much lower, and that of branch increment was much higher in the C. kawakamii than in the C. lanceolata (26.6% versus 40.3%, and 11.5% versus 0.3%). In both plantation, the above and belowground litterfall accounted 60% and 40% respectively for the annual litterfall production, which was estimated 7.183 t C·hm -2 a -1 in the C. kawakamii and 3.554 t C·hm -2 a -1 in the C. lanceolata. Annual soil heterotrophic respiration was responsible for a C loss of 5.983 and 2.984 t·hm -2 a -1 from soil to atmosphere in the C. kawakamii and the C. lanceolata, respectively. Carbon balance analysis showed there were a positive net ecosystem production (C sink), 7.656 and 3.615 t C·hm -2 a -1 , for the C. kawakamii and the C. lanceolata, respectively. For the purpose of carbon management, C. kawakamii might be a more suitable species than C. lanceolata in local region.
