The interaction between silicotungstic heteropolyacid and bovine hemoglobin (BHb) was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic ...The interaction between silicotungstic heteropolyacid and bovine hemoglobin (BHb) was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a major role in stabilizing the complex. The effect of silicotungstic heteropolyacid on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy.展开更多
The magnetic gelatin microspheres were prepared by means of reverse phase suspension method using Fe3O4 as core and gelatin as coating material.The magnetic Fe3O4 was prepared by precipitation.The products were charac...The magnetic gelatin microspheres were prepared by means of reverse phase suspension method using Fe3O4 as core and gelatin as coating material.The magnetic Fe3O4 was prepared by precipitation.The products were characterized by SEM,IR,and XRD.The results demonstrated that the diameter of Fe3O4 was in the range from 0.2 to 1.5 μm and the magnetic gelatin microspheres were spherical and smooth with the mean particle size in the range of 5～20μm.The sizes of microspheres were increased with an increase of the gelatin concentration.Some microspheres has irregular spherical.The magnetic gelatin microspheres had better magnetic quality with the saturation magnetization σs of 26.14emu.g-1,the coercivity Hc of 160Oe and residual magnetism σr of 0.26emu.g.展开更多
文摘The interaction between silicotungstic heteropolyacid and bovine hemoglobin (BHb) was investigated using fluorescence and UV-Vis. The experimental results show that the fluorescence quenching of BHb by silicotungstic heteropolyacid is a result of the formation of SiW-BHb complex; and the quenching mechanism is mainly static quenching. The binding site number n, apparent binding constant KA and corresponding thermodynamic parameters were measured at different temperatures. The process of binding SiW molecule on BHb was a spontaneous molecular interaction procedure in which entropy increased and Gibbs free energy decreased. Electrostatic interaction plays a major role in stabilizing the complex. The effect of silicotungstic heteropolyacid on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy.
文摘The magnetic gelatin microspheres were prepared by means of reverse phase suspension method using Fe3O4 as core and gelatin as coating material.The magnetic Fe3O4 was prepared by precipitation.The products were characterized by SEM,IR,and XRD.The results demonstrated that the diameter of Fe3O4 was in the range from 0.2 to 1.5 μm and the magnetic gelatin microspheres were spherical and smooth with the mean particle size in the range of 5～20μm.The sizes of microspheres were increased with an increase of the gelatin concentration.Some microspheres has irregular spherical.The magnetic gelatin microspheres had better magnetic quality with the saturation magnetization σs of 26.14emu.g-1,the coercivity Hc of 160Oe and residual magnetism σr of 0.26emu.g.
