从沙田柚自交花粉管中提取与自交不亲和相关蛋白,应用免疫胶体金标记抗体技术检测沙田柚Citrus grandis var. Shatinyu Hort.自花授粉后3 d 1/2花柱中花粉管特异蛋白的定位分布。结果表明,花粉管特异蛋白在花粉管中依次定位于内质网、...从沙田柚自交花粉管中提取与自交不亲和相关蛋白,应用免疫胶体金标记抗体技术检测沙田柚Citrus grandis var. Shatinyu Hort.自花授粉后3 d 1/2花柱中花粉管特异蛋白的定位分布。结果表明,花粉管特异蛋白在花粉管中依次定位于内质网、细胞质和细胞纤维壁上。展开更多
A method for immobilizing Bromophenol Blue(BPB)on glassy carbon electrode(GCE)was studied.BPB can be deposited on pretreated GCE by cyclic voltammetry.High acidity is favorable for the deposition.The formed electrode(...A method for immobilizing Bromophenol Blue(BPB)on glassy carbon electrode(GCE)was studied.BPB can be deposited on pretreated GCE by cyclic voltammetry.High acidity is favorable for the deposition.The formed electrode(BPB/GCE)gives a pair of well-defined peaks when scanned in phosphate buffer solution(PBS).Two electrons and two protons are involved in this process.The BPB/GCE shows an good stability and electroctalytic activity toward the oxidation of NADH in phosphate buffer solution.展开更多
文摘A method for immobilizing Bromophenol Blue(BPB)on glassy carbon electrode(GCE)was studied.BPB can be deposited on pretreated GCE by cyclic voltammetry.High acidity is favorable for the deposition.The formed electrode(BPB/GCE)gives a pair of well-defined peaks when scanned in phosphate buffer solution(PBS).Two electrons and two protons are involved in this process.The BPB/GCE shows an good stability and electroctalytic activity toward the oxidation of NADH in phosphate buffer solution.