In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU con...In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33ING1 were measured in vitro.The results were as follows: p33ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.The MTT results also proved that high expression of p33ING1 mRNA can increase cell’s sensitivity to chemical drug 5-FU.The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.展开更多
通过考察水与表面活性剂的摩尔比(R),TEOS的量、氨水的量及包壳次数对基于Triton X 100/环已烷/正已醇/水反相微乳液体系制备二氧化硅纳米颗粒尺寸的影响,开展了基于反相微乳液法的尺寸可控性二氧化硅纳米颗粒制备研究.结果表明:在其他...通过考察水与表面活性剂的摩尔比(R),TEOS的量、氨水的量及包壳次数对基于Triton X 100/环已烷/正已醇/水反相微乳液体系制备二氧化硅纳米颗粒尺寸的影响,开展了基于反相微乳液法的尺寸可控性二氧化硅纳米颗粒制备研究.结果表明:在其他参数都恒定的情况下,通过改变微乳液体系中上述某一组分的量,可以在一定程度上实现二氧化硅纳米颗粒的尺寸可控性合成.首先,水与表面活性剂的摩尔比(R)对二氧化硅纳米颗粒的尺寸影响最大,随着R值的增大,颗粒的粒径逐渐减小,当R值达到18时,二氧化硅纳米颗粒的形貌变得不再是很规则的球形结构,并且分散性降低,团聚现象明显;其次是氨水的量,随着氨水量的增多,颗粒的粒径先减小,之后不再发生明显变化;另外随着包壳次数的增多,颗粒的粒径随之增大,并且颗粒之间的分散性也有所提高;但是TEOS的量对颗粒粒径的影响不明显.展开更多
文摘In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay.During the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33ING1 were measured in vitro.The results were as follows: p33ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.The MTT results also proved that high expression of p33ING1 mRNA can increase cell’s sensitivity to chemical drug 5-FU.The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.
文摘通过考察水与表面活性剂的摩尔比(R),TEOS的量、氨水的量及包壳次数对基于Triton X 100/环已烷/正已醇/水反相微乳液体系制备二氧化硅纳米颗粒尺寸的影响,开展了基于反相微乳液法的尺寸可控性二氧化硅纳米颗粒制备研究.结果表明:在其他参数都恒定的情况下,通过改变微乳液体系中上述某一组分的量,可以在一定程度上实现二氧化硅纳米颗粒的尺寸可控性合成.首先,水与表面活性剂的摩尔比(R)对二氧化硅纳米颗粒的尺寸影响最大,随着R值的增大,颗粒的粒径逐渐减小,当R值达到18时,二氧化硅纳米颗粒的形貌变得不再是很规则的球形结构,并且分散性降低,团聚现象明显;其次是氨水的量,随着氨水量的增多,颗粒的粒径先减小,之后不再发生明显变化;另外随着包壳次数的增多,颗粒的粒径随之增大,并且颗粒之间的分散性也有所提高;但是TEOS的量对颗粒粒径的影响不明显.
