香榧细小卷蛾Lepteucosma torreyaeWu et Chen是危害香榧的主要害虫之一,在浙江省绍兴市1 a发生2代,以老熟幼虫在枯枝落叶中做茧越冬,翌年2月中旬化蛹,3月上旬成虫羽化并产卵,3月下旬第1代幼虫孵化,5月中旬化蛹,5月下旬成虫羽化,6月上...香榧细小卷蛾Lepteucosma torreyaeWu et Chen是危害香榧的主要害虫之一,在浙江省绍兴市1 a发生2代,以老熟幼虫在枯枝落叶中做茧越冬,翌年2月中旬化蛹,3月上旬成虫羽化并产卵,3月下旬第1代幼虫孵化,5月中旬化蛹,5月下旬成虫羽化,6月上旬产卵,6月中旬第2代幼虫孵化,11月上旬开始越冬。在第2代幼虫期,使用1.8%阿维菌素乳油、5%抑太保乳油、2%阿维苏云制剂、35%吡虫啉乳油及20%杀灭菊酯乳油等5种药剂10个浓度进行喷雾防治试验,施药9 d后,防治效果均达到100%。展开更多
The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis lar...The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis larvae was tested. The result showed that the optimum culture medium was PPDA,the optimum temperature for the mycelium growth and spore germination was 26 ℃,and A. glabripennis larvae had the highest mortality from the fungus at this temperature. The optimum RH for the mycelium growth ranged from 95%to 100%. The speed and rate of spore germination increased with increasing of RH. The larvae died from the fugus most quickly and the mortality reached the highest under 100% RH.展开更多
类受体激酶SOBIR1(suppressor of BIR1-1)在植物对病害的免疫应答中发挥重要作用。本研究在马铃薯中克隆了一个SOBIR1类似(StSOBIR1-like)基因,检测其在生物胁迫和非生物胁迫诱导下的表达特性;构建StSOBIR1-like基因沉默载体,并利用农...类受体激酶SOBIR1(suppressor of BIR1-1)在植物对病害的免疫应答中发挥重要作用。本研究在马铃薯中克隆了一个SOBIR1类似(StSOBIR1-like)基因,检测其在生物胁迫和非生物胁迫诱导下的表达特性;构建StSOBIR1-like基因沉默载体,并利用农杆菌介导法获得该基因的沉默株系;通过马铃薯块茎蛾生物测定实验和转录组测序,深入探究该基因沉默对马铃薯应答虫害的影响。结果表明:StSOBIR1-like基因在马铃薯叶片中高表达,马铃薯块茎蛾的模拟虫害、机械损伤以及外源施用茉莉酸甲酯、水杨酸、脱落酸等均能诱导该基因的表达,说明该基因可能参与马铃薯对生物胁迫和非生物胁迫诱导的生理反应。StSOBIR1-like沉默株系与野生型植株相比生长表型无明显差异,但马铃薯块茎蛾幼虫在前者上取食后的生物量比后者低,说明取食StSOBIR1-like沉默株系后马铃薯块茎蛾幼虫生长受到抑制。转录组测序结果表明,沉默StSOBIR1-like基因后,马铃薯植株的虫害应答在基因表达水平受到较大影响,特别是茉莉酸、水杨酸等防御激素合成通路相关基因的相对表达量总体呈上升趋势。综上所述,StSOBIR1-like基因可能负调控马铃薯对虫害的应答。本研究首次解析了StSOBIR1-like基因在马铃薯应答虫害中的功能,为进一步探究该基因调控作物抗虫的分子机制提供了依据。展开更多
文摘The effect of culture media,temperature and humidity on the mycelium growth,spores germination of Metarhizium MS01 strain,a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis larvae was tested. The result showed that the optimum culture medium was PPDA,the optimum temperature for the mycelium growth and spore germination was 26 ℃,and A. glabripennis larvae had the highest mortality from the fungus at this temperature. The optimum RH for the mycelium growth ranged from 95%to 100%. The speed and rate of spore germination increased with increasing of RH. The larvae died from the fugus most quickly and the mortality reached the highest under 100% RH.
文摘类受体激酶SOBIR1(suppressor of BIR1-1)在植物对病害的免疫应答中发挥重要作用。本研究在马铃薯中克隆了一个SOBIR1类似(StSOBIR1-like)基因,检测其在生物胁迫和非生物胁迫诱导下的表达特性;构建StSOBIR1-like基因沉默载体,并利用农杆菌介导法获得该基因的沉默株系;通过马铃薯块茎蛾生物测定实验和转录组测序,深入探究该基因沉默对马铃薯应答虫害的影响。结果表明:StSOBIR1-like基因在马铃薯叶片中高表达,马铃薯块茎蛾的模拟虫害、机械损伤以及外源施用茉莉酸甲酯、水杨酸、脱落酸等均能诱导该基因的表达,说明该基因可能参与马铃薯对生物胁迫和非生物胁迫诱导的生理反应。StSOBIR1-like沉默株系与野生型植株相比生长表型无明显差异,但马铃薯块茎蛾幼虫在前者上取食后的生物量比后者低,说明取食StSOBIR1-like沉默株系后马铃薯块茎蛾幼虫生长受到抑制。转录组测序结果表明,沉默StSOBIR1-like基因后,马铃薯植株的虫害应答在基因表达水平受到较大影响,特别是茉莉酸、水杨酸等防御激素合成通路相关基因的相对表达量总体呈上升趋势。综上所述,StSOBIR1-like基因可能负调控马铃薯对虫害的应答。本研究首次解析了StSOBIR1-like基因在马铃薯应答虫害中的功能,为进一步探究该基因调控作物抗虫的分子机制提供了依据。
