The effects of different concentrations of 5-fluorouracil, rifampicin and tetracycline on the phytoplasmas in the Paulownia tomentosa×Paulownia fortunei seedling and the pathogenic protein related to the witches...The effects of different concentrations of 5-fluorouracil, rifampicin and tetracycline on the phytoplasmas in the Paulownia tomentosa×Paulownia fortunei seedling and the pathogenic protein related to the witches’ broom symptom were studied with PCR based on 16S rRNA gene of the phytoplasmas and 2-dimensional electrophoresis of proteins. The results indicated that the plantlets in vitro culture treated with 0.5～300 mg·L -1 5-fluorouracil had little effect on the symptom remission, but those plantlets treated with 100 and 150 mg·L -1 rifampicin for 30 d showed obvious effect on the symptom remission respectively, and the phytoplasmas in the plantlets treated with 150 mg·L -1 rifampicin could not be detected with PCR of 16S rDNA of the phytoplasmas. Moreover, the protein (pI6.8,m 24 ku) which appeared in both the healthy seedling and diseases ones treated with 150 mg·L -1 rifampicin disappeared in the infected seedling and the those without the symptom after treating with rifampicin and tetracycline, and in which existed with phytoplasma through PCR detection.展开更多
The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the ...The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling（CK）,even the seedlings treated with lethal oxytetracycline concentration.展开更多
植物多倍化既是对自然环境适应的结果,也是推动其进化和物种形成的重要因素。自然界大约70%的被子植物在进化史中经历过一次或多次多倍化过程（Masterson,1994;Wendel,2000）。多倍体植物具有器官和生物量增大的特征及较强适应生物和非...植物多倍化既是对自然环境适应的结果,也是推动其进化和物种形成的重要因素。自然界大约70%的被子植物在进化史中经历过一次或多次多倍化过程（Masterson,1994;Wendel,2000）。多倍体植物具有器官和生物量增大的特征及较强适应生物和非生物胁迫的能力（Hilu,1993;Liu et al.,2002）。植物多倍化过程中,在染色体结构（Swapna et al.,展开更多
文摘The effects of different concentrations of 5-fluorouracil, rifampicin and tetracycline on the phytoplasmas in the Paulownia tomentosa×Paulownia fortunei seedling and the pathogenic protein related to the witches’ broom symptom were studied with PCR based on 16S rRNA gene of the phytoplasmas and 2-dimensional electrophoresis of proteins. The results indicated that the plantlets in vitro culture treated with 0.5～300 mg·L -1 5-fluorouracil had little effect on the symptom remission, but those plantlets treated with 100 and 150 mg·L -1 rifampicin for 30 d showed obvious effect on the symptom remission respectively, and the phytoplasmas in the plantlets treated with 150 mg·L -1 rifampicin could not be detected with PCR of 16S rDNA of the phytoplasmas. Moreover, the protein (pI6.8,m 24 ku) which appeared in both the healthy seedling and diseases ones treated with 150 mg·L -1 rifampicin disappeared in the infected seedling and the those without the symptom after treating with rifampicin and tetracycline, and in which existed with phytoplasma through PCR detection.
文摘The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling（CK）,even the seedlings treated with lethal oxytetracycline concentration.
基金National Natural Science Foundation of China(U1204309)Outstanding Talents Project of Henan Province(122101110700)+1 种基金Outstanding Talents Project of Henan Province(174200510001)Natural Science Foundation of Henan Province of China(162300410158)
文摘植物多倍化既是对自然环境适应的结果,也是推动其进化和物种形成的重要因素。自然界大约70%的被子植物在进化史中经历过一次或多次多倍化过程（Masterson,1994;Wendel,2000）。多倍体植物具有器官和生物量增大的特征及较强适应生物和非生物胁迫的能力（Hilu,1993;Liu et al.,2002）。植物多倍化过程中,在染色体结构（Swapna et al.,
