Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CD...Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art. Methods MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting. Results Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells. Conclusion Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating cell cycle through CDC25A down-regulation.展开更多
目的:探讨miR-200c在胃癌组织中的表达水平与胃癌患者临床病理特征及无病生存期(diease free survial,DFS)的关系。方法:收集河北医科大学第四医院普外科2012年5月至2013年1月64例胃癌手术切除的标本及相关临床资料,采用实时荧光定量PC...目的:探讨miR-200c在胃癌组织中的表达水平与胃癌患者临床病理特征及无病生存期(diease free survial,DFS)的关系。方法:收集河北医科大学第四医院普外科2012年5月至2013年1月64例胃癌手术切除的标本及相关临床资料,采用实时荧光定量PCR技术检测miR-200c在胃癌组织和配对癌旁非癌组织中的表达。回顾性分析miR-200c表达水平与胃癌患者的临床病理特征及DFS相关性。结果:胃癌组织中miR-200c的表达水平显著低于癌旁非癌组织(3.29 vs 5.91,P<0.01)。miR-200c的表达水平与肿瘤TNM分期、浸润深度、转移和脉管瘤栓呈显著负相关(均P<0.01)。miR-200c高表达组患者中位DFS明显长于低表达组患者(22.0 vs 13.5个月,P<0.01),其表达水平与患者DFS呈正相关(P<0.01)。结论:miR-200c在胃癌组织中低表达,其表达水平与肿瘤TNM分期、肿瘤浸润深度和脉管瘤栓呈负相关,与DFS呈正相关,在胃癌的发生发展及预后中具有重要作用。展开更多
文摘Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art. Methods MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting. Results Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells. Conclusion Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating cell cycle through CDC25A down-regulation.
文摘目的:检测人食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织及细胞中MicroRNA-203(miR-203)的表达及其基因的甲基化状态,探讨miR-203在ESCC发生及发展中的作用。方法:选取河北医科大学第四医院2008—2011年间手术切除的83例ESCC原发灶组织及癌旁组织标本,实时定量PCR与甲基化特异性PCR(methylation specific PCR,MSP)分别检测其miR-203的表达及其编码基因的甲基化状态。用DNA甲基化转移酶抑制剂5-氮杂-2’-脱氧胞苷(5-aza-2’-deoxycitydine,5-Aza-dC)处理食管癌细胞系(TE1、TE13、YES-2、EC109、T.TN),实时定量PCR与MSP分别检测5-Aza-dC处理对食管癌细胞中miR-203的表达及其基因甲基化状态的影响。结果:五种食管癌细胞中miR-203的表达均相对较低,且呈高甲基化状态。5-Aza-dC处理后,miR-203的表达均显著升高(P<0.05或P<0.01);YES-2细胞中miR-203编码基因的甲基化程度显著降低,其余4种细胞均转变为非甲基化状态。miR-203在ESCC组织中的表达显著低于癌旁组织(0.54±0.11 vs 1.00±0.01,P<0.01),启动子区甲基化率显著高于癌旁组织[62.65%(52/83)vs 7.23%(6/83),P<0.01],并且两者均与TNM分期和组织分化程度有关(P<0.05)。发生miR-203编码基因甲基化的ESCC组织中miR-203的表达显著低于未发生甲基化的ESCC组织(P<0.05)。结论:miR-203在ESCC组织与细胞中呈低表达,与食管鳞癌的发生、发展有关,且其启动子区甲基化可能是导致其表达沉默的机制之一。
文摘目的:探讨miR-200c在胃癌组织中的表达水平与胃癌患者临床病理特征及无病生存期(diease free survial,DFS)的关系。方法:收集河北医科大学第四医院普外科2012年5月至2013年1月64例胃癌手术切除的标本及相关临床资料,采用实时荧光定量PCR技术检测miR-200c在胃癌组织和配对癌旁非癌组织中的表达。回顾性分析miR-200c表达水平与胃癌患者的临床病理特征及DFS相关性。结果:胃癌组织中miR-200c的表达水平显著低于癌旁非癌组织(3.29 vs 5.91,P<0.01)。miR-200c的表达水平与肿瘤TNM分期、浸润深度、转移和脉管瘤栓呈显著负相关(均P<0.01)。miR-200c高表达组患者中位DFS明显长于低表达组患者(22.0 vs 13.5个月,P<0.01),其表达水平与患者DFS呈正相关(P<0.01)。结论:miR-200c在胃癌组织中低表达,其表达水平与肿瘤TNM分期、肿瘤浸润深度和脉管瘤栓呈负相关,与DFS呈正相关,在胃癌的发生发展及预后中具有重要作用。
