The change of glutamine synthetase (GS) isoform was determined by Native PAGE and the activity staining as well as Western blotting. The activity changes of GS, NADH dependent glutamate synthase (NADH GOGAT), and NADH...The change of glutamine synthetase (GS) isoform was determined by Native PAGE and the activity staining as well as Western blotting. The activity changes of GS, NADH dependent glutamate synthase (NADH GOGAT), and NADH dependent glutamate dehydrogenase (NADH GDH) were also analyzed by enzyme assay. The results indicateded that only one form of GS isozyme was detected during the germination of cushaw seed and the different development stages of the cotyledon grown in light or in darkness. GS activity was increaseed with the germination of the seed untill the cotyledon to begin green and then was reduced gradually. The tendency of the activity change of NADH GOGAT was similar to GS. The activity change of NADH GDH was contrary to that of GS and NADH GOGAT. The results suggested that the expression of GS isoform was not effected by the treatments of light and darkness in the experiment conditions. GS/GOGAT cycle may play a main role for assimilating ammonium during the germination of the seeds and the initial stage of the cotyledon development, and the cycle was weaken and GDH pathway was strengthened after the green of the cotyledon by comparing these enzyme activities.展开更多
莲藕组织富含多糖、脂质、酚类等物质,用一般的方法较难提取高质量的RNA。在改进前人方法的基础上,建立了一种高效、简单的CTAB-L iC l提取法,能快速提取高质量的莲藕组织总RNA,并且产率高、完整性好、纯度高,能进一步满足RT-PCR等分子...莲藕组织富含多糖、脂质、酚类等物质,用一般的方法较难提取高质量的RNA。在改进前人方法的基础上,建立了一种高效、简单的CTAB-L iC l提取法,能快速提取高质量的莲藕组织总RNA,并且产率高、完整性好、纯度高,能进一步满足RT-PCR等分子生物学实验的需要。此外,该方法也适用于其它富含多糖、脂质、酚类等物质的植物组织总RNA的提取。展开更多
文摘The change of glutamine synthetase (GS) isoform was determined by Native PAGE and the activity staining as well as Western blotting. The activity changes of GS, NADH dependent glutamate synthase (NADH GOGAT), and NADH dependent glutamate dehydrogenase (NADH GDH) were also analyzed by enzyme assay. The results indicateded that only one form of GS isozyme was detected during the germination of cushaw seed and the different development stages of the cotyledon grown in light or in darkness. GS activity was increaseed with the germination of the seed untill the cotyledon to begin green and then was reduced gradually. The tendency of the activity change of NADH GOGAT was similar to GS. The activity change of NADH GDH was contrary to that of GS and NADH GOGAT. The results suggested that the expression of GS isoform was not effected by the treatments of light and darkness in the experiment conditions. GS/GOGAT cycle may play a main role for assimilating ammonium during the germination of the seeds and the initial stage of the cotyledon development, and the cycle was weaken and GDH pathway was strengthened after the green of the cotyledon by comparing these enzyme activities.
文摘莲藕组织富含多糖、脂质、酚类等物质,用一般的方法较难提取高质量的RNA。在改进前人方法的基础上,建立了一种高效、简单的CTAB-L iC l提取法,能快速提取高质量的莲藕组织总RNA,并且产率高、完整性好、纯度高,能进一步满足RT-PCR等分子生物学实验的需要。此外,该方法也适用于其它富含多糖、脂质、酚类等物质的植物组织总RNA的提取。
