目的:筛查弱精子症患者精子中差异表达蛋白质,进行生物信息学分析,阐明重要的生物学过程、分子功能及相关的代谢通路,为进一步开展机制研究提供方向与思路。方法:分别收集正常人和弱精子症患者各30例的精子样本,利用串联质谱标签技术(Ta...目的:筛查弱精子症患者精子中差异表达蛋白质,进行生物信息学分析,阐明重要的生物学过程、分子功能及相关的代谢通路,为进一步开展机制研究提供方向与思路。方法:分别收集正常人和弱精子症患者各30例的精子样本,利用串联质谱标签技术(Tandem Mass Tag,TMT)筛查和鉴定弱精子症患者精子中的差异蛋白,并对其进行GO和KEGG分析。结果:以P<0.05,且表达倍数≥1.2或≤0.833为标准,与正常人组精子相比表达有差异的蛋白共1020种,上调蛋白606种,下调蛋白414种。GO分析结果显示上述差异表达蛋白主要参与mRNA分解过程、终止翻译过程,在核糖体和部分胞质均有分布,且具有结合功能、受体活性功能等;差异蛋白参与的KEGG信号通路为代谢通路、腺苷酸活化蛋白激酶(AMPK)信号通路和氧化磷酸化通路等257条通路。结论:弱精子症患者差异表达蛋白质涉及到复杂的生物学过程、功能与通路,为进一步开展弱精子症发病机制的研究提供了重要的方向与生物信息。展开更多
During the dissection of a 72-year old male cadaver,the hemiazygos vein(HAV)coursing the left side that drains into the persistent left superior vena cava was observed.The HAV was formed at the junction of the 9th to ...During the dissection of a 72-year old male cadaver,the hemiazygos vein(HAV)coursing the left side that drains into the persistent left superior vena cava was observed.The HAV was formed at the junction of the 9th to 11th right posterior intercostal veins,right subcostal vein,5th to 11th left posterior intercostal veins,and left subcostal vein;then ascended posteriorly to the thoracic aorta.After collecting the accessory hemiazygos vein,it crossed over the aorta and the pedicle of the left lung via the hemiazygos arch,then converged with a communicative branch(vein of Marshall)that emerged from the left brachiocephalic vein to form the persistent left superior vena cava and entered the pericardium.展开更多
文摘目的:筛查弱精子症患者精子中差异表达蛋白质,进行生物信息学分析,阐明重要的生物学过程、分子功能及相关的代谢通路,为进一步开展机制研究提供方向与思路。方法:分别收集正常人和弱精子症患者各30例的精子样本,利用串联质谱标签技术(Tandem Mass Tag,TMT)筛查和鉴定弱精子症患者精子中的差异蛋白,并对其进行GO和KEGG分析。结果:以P<0.05,且表达倍数≥1.2或≤0.833为标准,与正常人组精子相比表达有差异的蛋白共1020种,上调蛋白606种,下调蛋白414种。GO分析结果显示上述差异表达蛋白主要参与mRNA分解过程、终止翻译过程,在核糖体和部分胞质均有分布,且具有结合功能、受体活性功能等;差异蛋白参与的KEGG信号通路为代谢通路、腺苷酸活化蛋白激酶(AMPK)信号通路和氧化磷酸化通路等257条通路。结论:弱精子症患者差异表达蛋白质涉及到复杂的生物学过程、功能与通路,为进一步开展弱精子症发病机制的研究提供了重要的方向与生物信息。
文摘During the dissection of a 72-year old male cadaver,the hemiazygos vein(HAV)coursing the left side that drains into the persistent left superior vena cava was observed.The HAV was formed at the junction of the 9th to 11th right posterior intercostal veins,right subcostal vein,5th to 11th left posterior intercostal veins,and left subcostal vein;then ascended posteriorly to the thoracic aorta.After collecting the accessory hemiazygos vein,it crossed over the aorta and the pedicle of the left lung via the hemiazygos arch,then converged with a communicative branch(vein of Marshall)that emerged from the left brachiocephalic vein to form the persistent left superior vena cava and entered the pericardium.
