Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This resu...Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This result implied that the SH was the first example of the ion free artificial DNA cleavage agent that could split DNA by hydrolysis mechanism.展开更多
Nucleoside reverse transcriptase inhibitors are the only drugs so far approved for the treatment of AIDS. Several nucleoside analogs are potent inhibitors of human immunodeficiency virus(HIV) in cell culture. However,...Nucleoside reverse transcriptase inhibitors are the only drugs so far approved for the treatment of AIDS. Several nucleoside analogs are potent inhibitors of human immunodeficiency virus(HIV) in cell culture. However, in many cases the nucleoside derivatives have a poor affinity for nucleoside kinases. Nucleoside 5′-phosphorothioates is relatively resistant to enzymatic transformations. In this paper, 2′,3′-O-alkoxymethylidene adenosine 5′-thiophosphoramidates were synthesized through a highly efficient approach. The new compounds were characterized by NMR, IR and ESI-MS.展开更多
文摘Linear and supercoiled DNA were cleaved by HPLC purified seryl histidine dipeptide(SH). It was found that the DNA fragments produced by the reaction of SH and DNA could be ligated together by T 4 DNA ligase. This result implied that the SH was the first example of the ion free artificial DNA cleavage agent that could split DNA by hydrolysis mechanism.
文摘Nucleoside reverse transcriptase inhibitors are the only drugs so far approved for the treatment of AIDS. Several nucleoside analogs are potent inhibitors of human immunodeficiency virus(HIV) in cell culture. However, in many cases the nucleoside derivatives have a poor affinity for nucleoside kinases. Nucleoside 5′-phosphorothioates is relatively resistant to enzymatic transformations. In this paper, 2′,3′-O-alkoxymethylidene adenosine 5′-thiophosphoramidates were synthesized through a highly efficient approach. The new compounds were characterized by NMR, IR and ESI-MS.
