A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,t...A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.展开更多
A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to ...A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercritical carbon dioxide,and HPLC was used to analyze the transferred sample.The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum.The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined,which accorded with the result obtained with off-line extraction.Using this system,the whole process can be monitored and the optimum extraction time can be determined with single run.In addition,it has many other advantages,such as low sample loading,high sensitivity and accuracy,short analysis time,avoiding sample pollution and easily operation etc.The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.展开更多
本实验研究了昆仑雪菊总黄酮(Kunlun chrysanthemum total flavonoids,KCTF)的抗氧化活性、抑菌性、对四氯化碳(CCl4)诱导的小鼠肝损伤的保护作用,及对肿瘤细胞增殖的抑制能力。通过分析KCTF的自由基清除能力及对体内超氧化物歧化酶活...本实验研究了昆仑雪菊总黄酮(Kunlun chrysanthemum total flavonoids,KCTF)的抗氧化活性、抑菌性、对四氯化碳(CCl4)诱导的小鼠肝损伤的保护作用,及对肿瘤细胞增殖的抑制能力。通过分析KCTF的自由基清除能力及对体内超氧化物歧化酶活力和丙二醛含量的影响评价其抗氧化能力;采用CCl4诱导建立小鼠急性肝损伤模型检测KCTF的解毒作用;选取HeLa细胞和Eca-109食管癌细胞观察KCTF对肿瘤细胞增殖的抑制能力。结果表明:KCTF具有显著的抗氧化活性;KCTF能够抑制革兰氏阳性和革兰氏阴性菌的生长;然而KCTF对CCl4诱导的小鼠急性肝损伤没有明显的保护作用;KCTF能够抑制HeLa细胞和Eca-109细胞的增殖,半抑制质量浓度平均值分别为(133.60±0.12)、(192.00±0.08)μg/mL。结论:KCTF由于其天然的抗氧化、抑菌和抑制肿瘤细胞增殖作用,在功能性食品工业中具有潜在的应用价值。展开更多
文摘A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.
文摘A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercritical carbon dioxide,and HPLC was used to analyze the transferred sample.The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum.The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined,which accorded with the result obtained with off-line extraction.Using this system,the whole process can be monitored and the optimum extraction time can be determined with single run.In addition,it has many other advantages,such as low sample loading,high sensitivity and accuracy,short analysis time,avoiding sample pollution and easily operation etc.The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.
文摘本实验研究了昆仑雪菊总黄酮(Kunlun chrysanthemum total flavonoids,KCTF)的抗氧化活性、抑菌性、对四氯化碳(CCl4)诱导的小鼠肝损伤的保护作用,及对肿瘤细胞增殖的抑制能力。通过分析KCTF的自由基清除能力及对体内超氧化物歧化酶活力和丙二醛含量的影响评价其抗氧化能力;采用CCl4诱导建立小鼠急性肝损伤模型检测KCTF的解毒作用;选取HeLa细胞和Eca-109食管癌细胞观察KCTF对肿瘤细胞增殖的抑制能力。结果表明:KCTF具有显著的抗氧化活性;KCTF能够抑制革兰氏阳性和革兰氏阴性菌的生长;然而KCTF对CCl4诱导的小鼠急性肝损伤没有明显的保护作用;KCTF能够抑制HeLa细胞和Eca-109细胞的增殖,半抑制质量浓度平均值分别为(133.60±0.12)、(192.00±0.08)μg/mL。结论:KCTF由于其天然的抗氧化、抑菌和抑制肿瘤细胞增殖作用,在功能性食品工业中具有潜在的应用价值。
