The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the frac...The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.展开更多
A rapid method for the determination of morphine-type drugs in urine was established by SPE/GC-MS,and was applied in the real samples with satisfactory results.
To establish a convenient and efficient SPME/GC-MS method for the determination of terbufos in mulberry leaf.Samples were extracted by HS/SPME and analyzed by GC-MS.Mean recoveries ranged from(90±5.7)%.The method...To establish a convenient and efficient SPME/GC-MS method for the determination of terbufos in mulberry leaf.Samples were extracted by HS/SPME and analyzed by GC-MS.Mean recoveries ranged from(90±5.7)%.The method was simple,rapid,accurate,and was suitable for qualititative analysis of terbufos residue in mulberry leaf.展开更多
文摘The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.
文摘A rapid method for the determination of morphine-type drugs in urine was established by SPE/GC-MS,and was applied in the real samples with satisfactory results.
文摘To establish a convenient and efficient SPME/GC-MS method for the determination of terbufos in mulberry leaf.Samples were extracted by HS/SPME and analyzed by GC-MS.Mean recoveries ranged from(90±5.7)%.The method was simple,rapid,accurate,and was suitable for qualititative analysis of terbufos residue in mulberry leaf.
