目的:调查广西黑衣壮族人群4个短串联重复序列(short tandem repeats,STR)基因座:D6S477、D18S865、D19S400、D20S161的群体遗传分布资料。方法:应用PCR扩增结合聚丙烯酰胺凝胶电泳技术分析180名广西黑衣壮族无关个体。结果:4个STR基因...目的:调查广西黑衣壮族人群4个短串联重复序列(short tandem repeats,STR)基因座:D6S477、D18S865、D19S400、D20S161的群体遗传分布资料。方法:应用PCR扩增结合聚丙烯酰胺凝胶电泳技术分析180名广西黑衣壮族无关个体。结果:4个STR基因座平均观察杂合度分别为0.8361;平均多态信息总量为0.8047;累积个体识别力达0.999 988 391,累积非父排除率达0.988 207。在D19S400、D20S161基因座上广西黑衣壮族与广西武鸣壮族、广州汉族与青岛汉族的等位基因频率分布差异均无统计学意义;广西黑衣壮族与广西武鸣壮族遗传距离为0.0304。结论:D6S477的等位基因15、D18S865的等位基因11、D19SA00的等位基因12、D20S161的等位基因18可能是广西黑衣壮族群体中相应STR基因座最原始的等位基因。广西黑衣壮族的4个STR基因座属高度遗传多态性标记。同一民族内不同支系的遗传关系近于不同民族间的遗传关系。展开更多
The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the frac...The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.展开更多
文摘The metabolites and metabolic passways of nitrazepam in rat were confirmed.Wistar rats were feed a pill of nitrazepam,24 h urine reactions were collected.After β-Glucuronidase hydrolysis of the urine samples,the fractions were extracted by Oasis HLB3cc solid-phase column and analyzed by gas chromatography-mass spectrometry with DB-35 MS column.7-Acetylaminonitrazepam,7-aminonitrazepam and 2-amino-5-nitrophenylphenylmethanone were identified as nitrazepam metabolites.The results suggested that two metabolic passways for nitrazepam may be operative in rat.The first passway leads to the corresponding 7-aminonimetazepam in which the amino group is subsequently acetylated.The second passway is open the parent compounds rings to 2-amino-5-nitrophenylphenylmethanone.Nitrazepam was metabolized quickly in rats and 7-acetylaminonitrazepam were the main metabolites in urine.
