By successive chromatography on CM-Sepharose CL-6B,Sephadex G-100,Heparin-Sepharose CL-6B, a phosphodiesterase (PDE, EC3.1.4.1) was isolated and purified from Guangxi cobra venom. The final preparation was 89 fold pur...By successive chromatography on CM-Sepharose CL-6B,Sephadex G-100,Heparin-Sepharose CL-6B, a phosphodiesterase (PDE, EC3.1.4.1) was isolated and purified from Guangxi cobra venom. The final preparation was 89 fold pure and had high specific activity. The enzyme showed a single band on SDS-PAGE and IEF. The PDE showed a molecular weight of 110 kD and pI of 7.4. The enzyme consists of 840 amino acid residues and has only a methionine as N-terminal amino acid residues. It was rich in asparagine, leucine and glutamine and poor in sulphur-containing amino acid. It contains 5.38% neutral sugars. It has no acute toxicity and has analgesia effect.展开更多
目的:探讨激素性股骨头坏死(steroid induced osteonecrosis of the femoral head,SONFH)的血清蛋白质组学差异,寻找SONFH的血清特异性标志物。方法:分别收集SONFH患者(实验组)及正常体检者(对照组)各15例,同组血清等量混合,应用双向凝...目的:探讨激素性股骨头坏死(steroid induced osteonecrosis of the femoral head,SONFH)的血清蛋白质组学差异,寻找SONFH的血清特异性标志物。方法:分别收集SONFH患者(实验组)及正常体检者(对照组)各15例,同组血清等量混合,应用双向凝胶电泳串联基质辅助激光解离飞行时间质谱技术,寻找并鉴定差异表达的蛋白质。结果:共鉴定出9种差异蛋白质,与对照组比较,实验组中血清淀粉样蛋白A-2、凝血酶原、补体因子B、免疫球蛋白J链、富亮氨酸α-2-糖蛋白、补体C4、补体H等7种蛋白表达上调,肌动蛋白、载脂蛋白L1等2种蛋白表达下调。结论:运用蛋白质组学技术,成功发现了SONFH的血清差异表达蛋白质,为寻找SONFH特异性标志物及进一步阐明SONFH的发病机制提供了依据。展开更多
文摘By successive chromatography on CM-Sepharose CL-6B,Sephadex G-100,Heparin-Sepharose CL-6B, a phosphodiesterase (PDE, EC3.1.4.1) was isolated and purified from Guangxi cobra venom. The final preparation was 89 fold pure and had high specific activity. The enzyme showed a single band on SDS-PAGE and IEF. The PDE showed a molecular weight of 110 kD and pI of 7.4. The enzyme consists of 840 amino acid residues and has only a methionine as N-terminal amino acid residues. It was rich in asparagine, leucine and glutamine and poor in sulphur-containing amino acid. It contains 5.38% neutral sugars. It has no acute toxicity and has analgesia effect.
文摘目的:探讨通过增强树突状细胞(dendritic cells,DC)与T细胞的相互作用来进一步增强DC介导的抗肿瘤免疫效果。方法:体外培养的小鼠骨髓DC体外经携带人MIP-1β基因的重组腺病毒(adenovirus expressing human macrophoge irrflam—matory protein-1 beta,AdhMIP-1β)转染后(MIP-1β-DC),用小鼠CT26结肠腺癌细胞相关抗原冲击致敏,然后免疫正常同系小鼠,观察其体内诱导的细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)的保护性免疫反应;通过体内阻断试验探讨免疫细胞亚群及免疫分子在DC诱导抗肿瘤免疫应答中的作用。结果:经抗原致敏的MIP-1β—DC能更有效地诱导特异CTL活性,能使免疫动物产生更有效的免疫保护作用,抵抗肿瘤细胞的攻击。通过对其抗肿瘤免疫机理的分析发现,CD4+、CD8+T细胞共同参与了经抗原致敏的:MIP-1β—DC介导的抗肿瘤免疫反应,是主要的抗瘤效应细胞,NK细胞作用不明显。结论:通过基因修饰增强树突状细胞对T细胞的体内趋化活性,能更有效地诱导抗肿瘤免疫反应,为树突状细胞介导的肿瘤免疫基因治疗开辟了新的途径。
文摘目的:探讨激素性股骨头坏死(steroid induced osteonecrosis of the femoral head,SONFH)的血清蛋白质组学差异,寻找SONFH的血清特异性标志物。方法:分别收集SONFH患者(实验组)及正常体检者(对照组)各15例,同组血清等量混合,应用双向凝胶电泳串联基质辅助激光解离飞行时间质谱技术,寻找并鉴定差异表达的蛋白质。结果:共鉴定出9种差异蛋白质,与对照组比较,实验组中血清淀粉样蛋白A-2、凝血酶原、补体因子B、免疫球蛋白J链、富亮氨酸α-2-糖蛋白、补体C4、补体H等7种蛋白表达上调,肌动蛋白、载脂蛋白L1等2种蛋白表达下调。结论:运用蛋白质组学技术,成功发现了SONFH的血清差异表达蛋白质,为寻找SONFH特异性标志物及进一步阐明SONFH的发病机制提供了依据。
