The inhibitory effects of methyl cinnamate on the monophenolase activity and diphenolase activity of tyrosinase were studied by enzymological kinetic method in Na2HPO4-NaH2PO4 buffer solution(pH=6.8) at 30 ℃.Methyl c...The inhibitory effects of methyl cinnamate on the monophenolase activity and diphenolase activity of tyrosinase were studied by enzymological kinetic method in Na2HPO4-NaH2PO4 buffer solution(pH=6.8) at 30 ℃.Methyl cinnamate was found to inhibit the monophenolase activity and diphenolase activity of tyrosinase.The methyl cinnamate concentration leading to 50 % inhibitory rate(IC50) were 0.61 mmol/L for monophenolase activity and 1.49 mmol/L for diphenolase activity,respectively.Methyl cinnamate coued extend the lag time of monophenolase.0.8 mmol/L of methyl cinnamate resulted in the lag time extension from 1.1 min to 3.2 min.The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that methyl cinnamate was a noncompetitive inhibitor to diphenolase,and the inhibition constant KI for inhibitor binding with enzyme(E)was 0.66 mmol/L.展开更多
将罗非鱼的酶解产物及其超滤组分（〈5 k D酶解肽）加入罗非鱼鱼糜制品中,研究其在冷藏（0~4℃）期间对油脂和蛋白质的抗氧化效果。结果表明：酶解产物及超滤组分均可以改善鱼糜的色泽（P〈0.05）,尤其是超滤组分的效果接近于一定剂量的...将罗非鱼的酶解产物及其超滤组分（〈5 k D酶解肽）加入罗非鱼鱼糜制品中,研究其在冷藏（0~4℃）期间对油脂和蛋白质的抗氧化效果。结果表明：酶解产物及超滤组分均可以改善鱼糜的色泽（P〈0.05）,尤其是超滤组分的效果接近于一定剂量的VC;酶解产物对鱼糜中的油脂、蛋白质均表现出较强的抗氧化能力（P〈0.05）,其中超滤组分优于酶解液;酶解肽可以有效地保持鱼糜在冷藏期间的品质,超滤分离富集的〈5 k D的小分子肽显示出更好的抗氧化效果。展开更多
文摘The inhibitory effects of methyl cinnamate on the monophenolase activity and diphenolase activity of tyrosinase were studied by enzymological kinetic method in Na2HPO4-NaH2PO4 buffer solution(pH=6.8) at 30 ℃.Methyl cinnamate was found to inhibit the monophenolase activity and diphenolase activity of tyrosinase.The methyl cinnamate concentration leading to 50 % inhibitory rate(IC50) were 0.61 mmol/L for monophenolase activity and 1.49 mmol/L for diphenolase activity,respectively.Methyl cinnamate coued extend the lag time of monophenolase.0.8 mmol/L of methyl cinnamate resulted in the lag time extension from 1.1 min to 3.2 min.The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that methyl cinnamate was a noncompetitive inhibitor to diphenolase,and the inhibition constant KI for inhibitor binding with enzyme(E)was 0.66 mmol/L.
文摘将罗非鱼的酶解产物及其超滤组分（〈5 k D酶解肽）加入罗非鱼鱼糜制品中,研究其在冷藏（0~4℃）期间对油脂和蛋白质的抗氧化效果。结果表明：酶解产物及超滤组分均可以改善鱼糜的色泽（P〈0.05）,尤其是超滤组分的效果接近于一定剂量的VC;酶解产物对鱼糜中的油脂、蛋白质均表现出较强的抗氧化能力（P〈0.05）,其中超滤组分优于酶解液;酶解肽可以有效地保持鱼糜在冷藏期间的品质,超滤分离富集的〈5 k D的小分子肽显示出更好的抗氧化效果。
