Transcription factor NF-κB has attracted attention due to its important role in the regulation of the expression of a number of cellular genes involved in host defense,inflammation/immune responses,cytokine and growt...Transcription factor NF-κB has attracted attention due to its important role in the regulation of the expression of a number of cellular genes involved in host defense,inflammation/immune responses,cytokine and growth factor regulation,cell survival,proliferation,and also in embryonic development and programmed cell death. In this review,we provide an overview of established TNF-α,TLR/IL-1R,TCR and BCR signaling pathways to NF-κB,the alternative NF-κB pathway,and the regulation of NF-κB transcriptional activity. Furthermore,we discuss on emphasis the cross-talks between NF-κB and other signaling pathways (TGF-β,p53,nuclear receptor family,Ras/MEK/MAPK...),which are very complex and remain to be understood. The activation or inhibition of NF-κB has effect on other signaling pathways. They compose a complex signaling network,in which many regulatory signals integrate systematically and coordinate transcriptional responses to the stimulations. So when NF-κB is used as an important target of drugs in human diseases,the pathological and physiological functions of related signal pathways also should be understood sufficiently.展开更多
【目的】了解唾液抗幽门螺杆菌(Hp)抗体诊断 Hp 感染的价值。【方法】对93例病人同时进行唾液抗 HpIgG测定、快速尿素酶试验(RUT)、组织涂片 HE 染色及病理 WS 银染色。【结果】唾液抗 HpIgG 检测阳性率近似于 RUT 法(P>0.05),高于...【目的】了解唾液抗幽门螺杆菌(Hp)抗体诊断 Hp 感染的价值。【方法】对93例病人同时进行唾液抗 HpIgG测定、快速尿素酶试验(RUT)、组织涂片 HE 染色及病理 WS 银染色。【结果】唾液抗 HpIgG 检测阳性率近似于 RUT 法(P>0.05),高于组织涂片 HE 染色及病理 WS 银染色法(P<0.01)。其诊断 Hp 感染的敏感性为97.0%,特异性为88.2%,准确性为95.2%,阳性预期值97.0%,阴性预期值88.2%。【结论】本方法与组织学方法比较具有更高的敏感性,且简便、快速、可靠。展开更多
目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PC...目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PCR检测NPAS2 m RNA在40例结直肠癌及癌旁新鲜组织中的表达;采用免疫组织化学SABC法检测NPAS2蛋白在120例肿瘤及癌旁石蜡标本中的表达;利用其中78例随访患者资料,K-M生存法分析NPAS2蛋白表达水平与患者生存率之间的相关性。结果 RT-q PCR结果提示,结直肠癌中NPAS2 m RNA表达显著低于相对应的癌旁组织(P<0.01);免疫组织化学结果提示,NPAS2蛋白表达在结直肠癌组织中的阳性率为19.2%,显著低于癌旁中的62.5%(P<0.05)。NPAS2的低表达与结直肠癌的肿瘤大小、淋巴结转移及TNM分期密切相关(P<0.05),而与患者年龄、性别、分化、浸润深度及远处转移无显著相关性(P>0.05)。K-M生存分析结果表明,NPAS2高表达组患者5年生存率显著高于低表达组,差异具有统计学意义(P<0.01)。结论NPAS2在结直肠癌组织中低表达,且与肿瘤部分恶性生物学行为及患者5年生存率密切相关,提示检测NPAS2的表达对预测结直肠癌患者预后具有重要意义。展开更多
文摘Transcription factor NF-κB has attracted attention due to its important role in the regulation of the expression of a number of cellular genes involved in host defense,inflammation/immune responses,cytokine and growth factor regulation,cell survival,proliferation,and also in embryonic development and programmed cell death. In this review,we provide an overview of established TNF-α,TLR/IL-1R,TCR and BCR signaling pathways to NF-κB,the alternative NF-κB pathway,and the regulation of NF-κB transcriptional activity. Furthermore,we discuss on emphasis the cross-talks between NF-κB and other signaling pathways (TGF-β,p53,nuclear receptor family,Ras/MEK/MAPK...),which are very complex and remain to be understood. The activation or inhibition of NF-κB has effect on other signaling pathways. They compose a complex signaling network,in which many regulatory signals integrate systematically and coordinate transcriptional responses to the stimulations. So when NF-κB is used as an important target of drugs in human diseases,the pathological and physiological functions of related signal pathways also should be understood sufficiently.
文摘【目的】了解唾液抗幽门螺杆菌(Hp)抗体诊断 Hp 感染的价值。【方法】对93例病人同时进行唾液抗 HpIgG测定、快速尿素酶试验(RUT)、组织涂片 HE 染色及病理 WS 银染色。【结果】唾液抗 HpIgG 检测阳性率近似于 RUT 法(P>0.05),高于组织涂片 HE 染色及病理 WS 银染色法(P<0.01)。其诊断 Hp 感染的敏感性为97.0%,特异性为88.2%,准确性为95.2%,阳性预期值97.0%,阴性预期值88.2%。【结论】本方法与组织学方法比较具有更高的敏感性,且简便、快速、可靠。
文摘目的检测生物节律基因NPAS2在结直肠癌组织及相应癌旁组织中的表达情况;明确NPAS2的表达与患者临床病理学参数之间的相关性,分析NPAS2表达与结直肠癌患者5年生存率之间的相关性,明确其在判断结直肠癌患者预后中的作用。方法采用RT-q PCR检测NPAS2 m RNA在40例结直肠癌及癌旁新鲜组织中的表达;采用免疫组织化学SABC法检测NPAS2蛋白在120例肿瘤及癌旁石蜡标本中的表达;利用其中78例随访患者资料,K-M生存法分析NPAS2蛋白表达水平与患者生存率之间的相关性。结果 RT-q PCR结果提示,结直肠癌中NPAS2 m RNA表达显著低于相对应的癌旁组织(P<0.01);免疫组织化学结果提示,NPAS2蛋白表达在结直肠癌组织中的阳性率为19.2%,显著低于癌旁中的62.5%(P<0.05)。NPAS2的低表达与结直肠癌的肿瘤大小、淋巴结转移及TNM分期密切相关(P<0.05),而与患者年龄、性别、分化、浸润深度及远处转移无显著相关性(P>0.05)。K-M生存分析结果表明,NPAS2高表达组患者5年生存率显著高于低表达组,差异具有统计学意义(P<0.01)。结论NPAS2在结直肠癌组织中低表达,且与肿瘤部分恶性生物学行为及患者5年生存率密切相关,提示检测NPAS2的表达对预测结直肠癌患者预后具有重要意义。
