A liquid chsomatographic-tandem mass spectrometric method was used to simultaneously determine four tobacco-specific nitrosamines in cigarette mainstream smoke.Cigarette smoke particulate,obtained under standardized m...A liquid chsomatographic-tandem mass spectrometric method was used to simultaneously determine four tobacco-specific nitrosamines in cigarette mainstream smoke.Cigarette smoke particulate,obtained under standardized machine smoking,was collected on a Cambridge filter pad.The particulate was co-extracted with citric acid-phosphate buffer and cyclohexane and further purified by solid-phase extraction.Analyte identification and quantification were performed using multiple reaction monitoring with one precursor ion and two product ions as identifiers and electrospray ionization in positive mode.The limits of detection were in the range of 0.05～0.24ng/mL using an injection volume of 20μL,linear range over 2.5～1000ng/mL,linear correlation coefficients above 0.998,and recoveries 90.2%～95.1%.展开更多
Polyphenols in seventeen Yunnan flue-cured tobacco leaves were assayed by high performance liquid chromatography(HPLC).The major seven tobacco polyphenols were quantitatively determined.HPLC polyphenol profiles were p...Polyphenols in seventeen Yunnan flue-cured tobacco leaves were assayed by high performance liquid chromatography(HPLC).The major seven tobacco polyphenols were quantitatively determined.HPLC polyphenol profiles were processed by pattern recognition(PRM).The values of color similarity criterion TK among these tobacco leaves were calculated and compared with human organoleptic color assessment.It was shown that data from PRM based on HPLC profiles of polyphenols could be used to quantitatively characterize color similarity among Yunnan tobacco leaves of different grades.The results by PRM and by organoleptic assessment were compatible.The former was more objective.展开更多
文摘A liquid chsomatographic-tandem mass spectrometric method was used to simultaneously determine four tobacco-specific nitrosamines in cigarette mainstream smoke.Cigarette smoke particulate,obtained under standardized machine smoking,was collected on a Cambridge filter pad.The particulate was co-extracted with citric acid-phosphate buffer and cyclohexane and further purified by solid-phase extraction.Analyte identification and quantification were performed using multiple reaction monitoring with one precursor ion and two product ions as identifiers and electrospray ionization in positive mode.The limits of detection were in the range of 0.05～0.24ng/mL using an injection volume of 20μL,linear range over 2.5～1000ng/mL,linear correlation coefficients above 0.998,and recoveries 90.2%～95.1%.
文摘Polyphenols in seventeen Yunnan flue-cured tobacco leaves were assayed by high performance liquid chromatography(HPLC).The major seven tobacco polyphenols were quantitatively determined.HPLC polyphenol profiles were processed by pattern recognition(PRM).The values of color similarity criterion TK among these tobacco leaves were calculated and compared with human organoleptic color assessment.It was shown that data from PRM based on HPLC profiles of polyphenols could be used to quantitatively characterize color similarity among Yunnan tobacco leaves of different grades.The results by PRM and by organoleptic assessment were compatible.The former was more objective.
