In this paper, by applying fluorescence quenching method and energy transfer technique, the interaction of serum albumin(BSA and HSA) with ketorolac and feprazone was investigated. The binding sites and binding consta...In this paper, by applying fluorescence quenching method and energy transfer technique, the interaction of serum albumin(BSA and HSA) with ketorolac and feprazone was investigated. The binding sites and binding constants were obtained. According to the thermodynamic parameters, the main sorts of binding force can be known. The results show that two drugs and protein have strong interactions. The mechanism of quenching belongs to static quenching and the main sort of binding force is static electricity gravitation.展开更多
Inclusion complex of ferrocene(Fc) with β-cyclodextrin(β-CD) has been synthesized in ethylene glycol. It was unsolvable in water and had been successfully used in the preparation of β-CD-Fc inclusion complex modifi...Inclusion complex of ferrocene(Fc) with β-cyclodextrin(β-CD) has been synthesized in ethylene glycol. It was unsolvable in water and had been successfully used in the preparation of β-CD-Fc inclusion complex modified carbon paste electrode (CFCPE). Using the inclusion complex as the electroactive substance greatly increased the stability and reproducibility of CFCPE than using Fc.展开更多
A new fluorescent reagent, salicylaldehyde-5-bromo-salicyloyhydrazone (SABSH ), was synthesized and its ionization constants were established spectropho-tometrically. The fluorescent reaction of this reagent with scan...A new fluorescent reagent, salicylaldehyde-5-bromo-salicyloyhydrazone (SABSH ), was synthesized and its ionization constants were established spectropho-tometrically. The fluorescent reaction of this reagent with scandium was studied in detail.Based on this chelation reaction, a spectrofluorimetric method was developed for the determi-nation of scandium in a water-ethanol(1:1, V/V) medium at pH 4. 8. Under these condi-tions, the Sc-SABSH complex has excitation and emission maxima at 392 and 466 nm, re-spectively. The linear range of the method is from 0.0 to 1.0μg/10 mL, the relative stan-dard deviation of the method is 0.95% and the detection limit is o. 15 ng/mL of scandium when a standard additions method is used in the assay. The molar ratlo of scandium to reagent is 1: 1. Interferences of forty other ions were studied. The method has been suc-cessfully applied to the determination of trace scandium in geologic samples-展开更多
In this paper, thermograms of eight bacteria and growth ctirves in exponential phase of growth are presented. Growth rate constants and their temperature dependent non-llnear equations axe also established. The optimu...In this paper, thermograms of eight bacteria and growth ctirves in exponential phase of growth are presented. Growth rate constants and their temperature dependent non-llnear equations axe also established. The optimu-m growth temperattire of these bacteria can be easily caJculated from such equations. It is very informative for the study of microorganism growth.展开更多
文摘In this paper, by applying fluorescence quenching method and energy transfer technique, the interaction of serum albumin(BSA and HSA) with ketorolac and feprazone was investigated. The binding sites and binding constants were obtained. According to the thermodynamic parameters, the main sorts of binding force can be known. The results show that two drugs and protein have strong interactions. The mechanism of quenching belongs to static quenching and the main sort of binding force is static electricity gravitation.
文摘Inclusion complex of ferrocene(Fc) with β-cyclodextrin(β-CD) has been synthesized in ethylene glycol. It was unsolvable in water and had been successfully used in the preparation of β-CD-Fc inclusion complex modified carbon paste electrode (CFCPE). Using the inclusion complex as the electroactive substance greatly increased the stability and reproducibility of CFCPE than using Fc.
文摘A new fluorescent reagent, salicylaldehyde-5-bromo-salicyloyhydrazone (SABSH ), was synthesized and its ionization constants were established spectropho-tometrically. The fluorescent reaction of this reagent with scandium was studied in detail.Based on this chelation reaction, a spectrofluorimetric method was developed for the determi-nation of scandium in a water-ethanol(1:1, V/V) medium at pH 4. 8. Under these condi-tions, the Sc-SABSH complex has excitation and emission maxima at 392 and 466 nm, re-spectively. The linear range of the method is from 0.0 to 1.0μg/10 mL, the relative stan-dard deviation of the method is 0.95% and the detection limit is o. 15 ng/mL of scandium when a standard additions method is used in the assay. The molar ratlo of scandium to reagent is 1: 1. Interferences of forty other ions were studied. The method has been suc-cessfully applied to the determination of trace scandium in geologic samples-
文摘In this paper, thermograms of eight bacteria and growth ctirves in exponential phase of growth are presented. Growth rate constants and their temperature dependent non-llnear equations axe also established. The optimu-m growth temperattire of these bacteria can be easily caJculated from such equations. It is very informative for the study of microorganism growth.
