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黄大茶多糖的结构表征及其对3T3-L1前脂肪细胞分化的抑制作用 被引量:1
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作者 徐珊 谢海 +5 位作者 李敏妮 马妍 葛慧芳 李大祥 谢忠稳 王红燕 《食品工业科技》 北大核心 2025年第8期67-75,共9页
本文探究黄大茶多糖的结构特征和降脂活性,为夏秋茶资源的开发利用提供依据。以霍山黄大茶为试验材料提取黄大茶多糖,对其结构进行表征,分别采用高效液相凝胶渗透色谱(High-performance gel-permeation chromatography,HPGPC)、离子色谱... 本文探究黄大茶多糖的结构特征和降脂活性,为夏秋茶资源的开发利用提供依据。以霍山黄大茶为试验材料提取黄大茶多糖,对其结构进行表征,分别采用高效液相凝胶渗透色谱(High-performance gel-permeation chromatography,HPGPC)、离子色谱(Ion chromatography,IC)、气质联用色谱(Gas chromatography-mass spectrometry,GC-MS)、傅里叶红外光谱(Fourier transform infrared spectrometer,FTIR)、扫描电镜(Scanning electron microscope,SEM)等分析方法测定黄大茶多糖的纯度、分子质量、单糖组成、化学键组成以及糖苷键结构;同时基于3T3-L1前脂肪细胞构建成熟脂肪细胞分化模型,研究黄大茶多糖干预对3T3-L1细胞分化和脂滴累积的影响。结果表明,黄大茶多糖经水提醇沉以及DE-52纤维素阴离子交换层析柱和Sephadex G-100凝胶柱纯化分离获得LYP-W1组分,HPGPC、IC和GC-MS分析结果显示,LYP-W1的分子量为1.25×105 Da,单糖组成为Ara:Rha:Gal:Glc:Man:GalA:GlcA为22.22:8.20:27.59:14.88:5.90:4.09,糖苷键主要由Rhap-(→1、Araf-(1→、→2)-Rhap-(1→、Glcp-(1→、GlcpA-(1→、Galp-(→1、→5)-Araf-(1→、→2,4)-Rhap-(1→、→4)-Galp-(→1、→4)-GalpA-(1→、→4)-Glcp-(1→、→3,4)-Manp-(1→、→3,6)-Galp-(1→等连接方式构成。FTIR结合SEM分析结果显示,LYPW1中具有β-型吡喃糖环,表面光滑致密,呈不规则片层薄膜状分布。细胞实验结果显示,LYP-W1在50~200μg/mL浓度范围内呈剂量依赖性显著抑制3T3-L1细胞的分化并降低其脂质沉积(P<0.05),表明黄大茶多糖具有天然降脂的潜力。本研究可为其它茶多糖的提取、纯化及生物活性研究提供参考。 展开更多
关键词 黄大茶多糖 分离纯化 结构鉴定 降脂活性
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Preparation of Biodegradable Polylactic Acid/Ethyl Cellulose/Zein Composite Film and Its Effect on the Preservation of Chilled Fresh Meat
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作者 ZHOU Ling YU Ya +8 位作者 YUAN Mengting WU Dongxu CHEN Ya LIU Yanan SU Jingjing CHEN Sihan WANG Juhua SHENG Bulei XUE Xiuheng 《食品科学》 北大核心 2025年第15期324-337,共14页
In this study,composite films consisting of polylactic acid(PLA),ethyl cellulose(EC),and zein were prepared by solution casting method,and their performance and application in chilled fresh meat preservation were inve... In this study,composite films consisting of polylactic acid(PLA),ethyl cellulose(EC),and zein were prepared by solution casting method,and their performance and application in chilled fresh meat preservation were investigated.The results showed that the three materials had satisfactory compatibility in the composite film.Addition of EC and zein effectively improved the mechanical properties,thermodynamic properties,surface hydrophilicity,oxygen permeability,and degradation properties of PLA films.When the ratio of PLA to EC was 3:7,the tensile strength and elongation at break reached maximum values of 16.6 MPa and 30.5%,respectively.Moreover,under different conditions,the composite film exhibited better degradability than the PLA film.The composite film with a 3:7 ratio of PLA to EC had the best performance,with a degradation rate of 21.75%after 84 days.Chilled fresh meat wrapped with the composite film showed significantly improved antioxidant,antibacterial,and water-holding properties. 展开更多
关键词 biodegradable film polylactic acid ethyl cellulose ZEIN chilled fresh meat PRESERVATION
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