Objective To investigate the causal relationships between plasma metabolites and osteoporosis via Mendelian randomization(MR) analysis.Methods Bidirectional MR was used to analyze pooled data from different genome-wid...Objective To investigate the causal relationships between plasma metabolites and osteoporosis via Mendelian randomization(MR) analysis.Methods Bidirectional MR was used to analyze pooled data from different genome-wide association studies(GWAS). The causal effect of plasma metabolites on osteoporosis was estimated using the inverse variance weighted method, intersections of statistically significant metabolites obtained from different sources of osteoporosis-related GWAS aggregated data was determined, and then sensitivity analysis was performed on these metabolites. Heterogeneity between single nucleotide polymorphisms was evaluated by Cochran's Q test. Horizontal pleiotropy was assessed through the application of the MR-Egger intercept method and the MRPRESSO method. The causal effect of osteoporosis on plasma metabolites was also evaluated using the inverse variance weighted method. Additionally, pathway analysis was conducted to identify potential metabolic pathways involved in the regulation of osteoporosis.Results Primary analysis and sensitivity analysis showed that 77 and 61 plasma metabolites had a causal relationship with osteoporosis from the GWAS data in the GCST90038656 and GCST90044600 datasets, respectively. Five common metabolites were identified via intersection. X-13684 levels and the glucose-to-maltose ratio were negatively associated with osteoporosis, whereas glycoursodeoxycholate levels and arachidoylcarnitine(C20) levels were positively associated with osteoporosis(all P < 0.05). The relationship between X-11299 levels and osteoporosis showed contradictory results(all P < 0.05). Pathway analysis indicated that glycine, serine, and threonine metabolism, valine, leucine, and isoleucine biosynthesis, galactose metabolism, arginine biosynthesis, and starch and sucrose metabolism pathways were participated in the development of osteoporosis.Conclusion We found a causal relationship between plasma metabolites and osteoporosis. These results offer novel perspectives with important implications for targeted metabolite-focused interventions in the management of osteoporosis.展开更多
目的观察养血活血、健脾补肾中药内服配合股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)内固定术治疗老年股骨粗隆间骨折的临床疗效。方法将56例股骨粗隆间骨折行手术治疗的老年患者随机分为观察组和对照组,每组28例。...目的观察养血活血、健脾补肾中药内服配合股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)内固定术治疗老年股骨粗隆间骨折的临床疗效。方法将56例股骨粗隆间骨折行手术治疗的老年患者随机分为观察组和对照组,每组28例。观察组采用加味桃红四物汤内服联合PFNA内固定术治疗,对照组仅行PFNA内固定术。采用Harris评分评价髋关节功能,观察并比较两组患者术后肿胀消退时间、骨折愈合时间和总并发症发生率。结果术后30、60 d,两组患者髋关节Harris评分逐渐升高,差异均有统计学意义(P<0.05),且观察组髋关节Harris评分显著高于对照组(P<0.05)。观察组患者骨折愈合时间和患肢肿胀消退时间较对照组明显缩短(P<0.05),总并发症发生率显著降低(P<0.05)。结论加味桃红四物汤内服配合PFNA内固定术可改善老年股骨粗隆间骨折患者髋关节功能,促进骨折愈合,减轻患肢肿胀,减少并发症发生率。展开更多
目的:系统评价活血通络类中药治疗神经根型颈椎病的有效性和安全性。方法:采用电子计算机检索Pub Med、CBM、CINK、VIP和WF等数据库,纳入活血通络类中药治疗神经根型颈椎病的随机对照试验(RCTs),所有检索均截止至2014年3月31日。由2名...目的:系统评价活血通络类中药治疗神经根型颈椎病的有效性和安全性。方法:采用电子计算机检索Pub Med、CBM、CINK、VIP和WF等数据库,纳入活血通络类中药治疗神经根型颈椎病的随机对照试验(RCTs),所有检索均截止至2014年3月31日。由2名评价者依据Cochrane系统评价员手册5.1中的风险偏倚评估工具独立评价纳入文献的方法学质量并提取有效数据并采用Rev Man 5.2软件分析数据。结果:本研究纳入13个RCTs,共1371例患者。Meta分析结果显示,研究主要以有效率为主要结局指标,结果显示口服活血通络类中药治疗神经根型颈椎病总有效率明显优于西药治疗,2组比较差异有统计学意义[n=13,RR=1.14,95%CI(1.09,1.19),P<0.00001]。结论:活血通络类中药治疗神经根型颈椎病疗效较西药治疗有一定优势,但鉴于本研究纳入的原始文献质量不高,有效性和安全性尚不能得肯定结论,开展更多大样本、多中心、随机双盲的方法科学和规范的高质量随机对照试验(RCT)非常必要。展开更多
目的构建桃红四物汤干预腰椎间盘突出症的“药物-靶基因-疾病”预测模型,探究桃红四物汤治疗腰椎间盘突出症的核心靶点及作用通路。方法通过中药系统药理学数据库与分析平台收集桃红四物汤中有效的化学成分及靶点信息,运用UniProt蛋白...目的构建桃红四物汤干预腰椎间盘突出症的“药物-靶基因-疾病”预测模型,探究桃红四物汤治疗腰椎间盘突出症的核心靶点及作用通路。方法通过中药系统药理学数据库与分析平台收集桃红四物汤中有效的化学成分及靶点信息,运用UniProt蛋白质数据库实现基因靶点名称规范化;从GeneCards、疗效靶点数据库、人类孟德尔遗传在线、DRUGBANK数据库中查找腰椎间盘突出症的靶点。构建“成分-靶点-疾病”网络,并对蛋白相互作用(protein-protein interaction,PPI)网络进行可视化分析,分析排名前20个靶点的生物过程、分子功能、细胞组成和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)中信号通路。结果筛选出69个桃红四物汤的有效化学成分,235个潜在基因靶点,其中与腰椎间盘突出症共同的基因靶点有82个。PPI网络分析显示,肿瘤坏死因子(tumor necrosis factor,TNF)、表皮生长因子(epidermal growth factor,EGF)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、激活蛋白1(activator protein 1,JUN)、丝氨酸/苏氨酸蛋白激酶(serine/threonine-protein kinase 1,AKT1)是桃红四物汤干预腰椎间盘突出症的核心靶点。基因本体论的富集分析结果显示,桃红四物汤干预腰椎间盘突出症的过程涉及对DNA结合转录因子活性的调节、细胞对生物刺激的反应、氧化应激反应以及对脂多糖、细菌来源分子的调节;KEGG的通路富集分析结果显示,丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)通路、晚期糖基化终末产物(advanced glycation end product,AGE)-AGE受体(receptor for AGE,RAGE)通路、TNF通路的差异蛋白表达水平较高。结论桃红四物汤可能是通过抗炎、提高免疫、调控激素、延缓细胞凋亡等作用多靶点、多通路调控腰椎间盘突出症。展开更多
文摘Objective To investigate the causal relationships between plasma metabolites and osteoporosis via Mendelian randomization(MR) analysis.Methods Bidirectional MR was used to analyze pooled data from different genome-wide association studies(GWAS). The causal effect of plasma metabolites on osteoporosis was estimated using the inverse variance weighted method, intersections of statistically significant metabolites obtained from different sources of osteoporosis-related GWAS aggregated data was determined, and then sensitivity analysis was performed on these metabolites. Heterogeneity between single nucleotide polymorphisms was evaluated by Cochran's Q test. Horizontal pleiotropy was assessed through the application of the MR-Egger intercept method and the MRPRESSO method. The causal effect of osteoporosis on plasma metabolites was also evaluated using the inverse variance weighted method. Additionally, pathway analysis was conducted to identify potential metabolic pathways involved in the regulation of osteoporosis.Results Primary analysis and sensitivity analysis showed that 77 and 61 plasma metabolites had a causal relationship with osteoporosis from the GWAS data in the GCST90038656 and GCST90044600 datasets, respectively. Five common metabolites were identified via intersection. X-13684 levels and the glucose-to-maltose ratio were negatively associated with osteoporosis, whereas glycoursodeoxycholate levels and arachidoylcarnitine(C20) levels were positively associated with osteoporosis(all P < 0.05). The relationship between X-11299 levels and osteoporosis showed contradictory results(all P < 0.05). Pathway analysis indicated that glycine, serine, and threonine metabolism, valine, leucine, and isoleucine biosynthesis, galactose metabolism, arginine biosynthesis, and starch and sucrose metabolism pathways were participated in the development of osteoporosis.Conclusion We found a causal relationship between plasma metabolites and osteoporosis. These results offer novel perspectives with important implications for targeted metabolite-focused interventions in the management of osteoporosis.
文摘目的观察养血活血、健脾补肾中药内服配合股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)内固定术治疗老年股骨粗隆间骨折的临床疗效。方法将56例股骨粗隆间骨折行手术治疗的老年患者随机分为观察组和对照组,每组28例。观察组采用加味桃红四物汤内服联合PFNA内固定术治疗,对照组仅行PFNA内固定术。采用Harris评分评价髋关节功能,观察并比较两组患者术后肿胀消退时间、骨折愈合时间和总并发症发生率。结果术后30、60 d,两组患者髋关节Harris评分逐渐升高,差异均有统计学意义(P<0.05),且观察组髋关节Harris评分显著高于对照组(P<0.05)。观察组患者骨折愈合时间和患肢肿胀消退时间较对照组明显缩短(P<0.05),总并发症发生率显著降低(P<0.05)。结论加味桃红四物汤内服配合PFNA内固定术可改善老年股骨粗隆间骨折患者髋关节功能,促进骨折愈合,减轻患肢肿胀,减少并发症发生率。
文摘目的:系统评价活血通络类中药治疗神经根型颈椎病的有效性和安全性。方法:采用电子计算机检索Pub Med、CBM、CINK、VIP和WF等数据库,纳入活血通络类中药治疗神经根型颈椎病的随机对照试验(RCTs),所有检索均截止至2014年3月31日。由2名评价者依据Cochrane系统评价员手册5.1中的风险偏倚评估工具独立评价纳入文献的方法学质量并提取有效数据并采用Rev Man 5.2软件分析数据。结果:本研究纳入13个RCTs,共1371例患者。Meta分析结果显示,研究主要以有效率为主要结局指标,结果显示口服活血通络类中药治疗神经根型颈椎病总有效率明显优于西药治疗,2组比较差异有统计学意义[n=13,RR=1.14,95%CI(1.09,1.19),P<0.00001]。结论:活血通络类中药治疗神经根型颈椎病疗效较西药治疗有一定优势,但鉴于本研究纳入的原始文献质量不高,有效性和安全性尚不能得肯定结论,开展更多大样本、多中心、随机双盲的方法科学和规范的高质量随机对照试验(RCT)非常必要。
文摘目的构建桃红四物汤干预腰椎间盘突出症的“药物-靶基因-疾病”预测模型,探究桃红四物汤治疗腰椎间盘突出症的核心靶点及作用通路。方法通过中药系统药理学数据库与分析平台收集桃红四物汤中有效的化学成分及靶点信息,运用UniProt蛋白质数据库实现基因靶点名称规范化;从GeneCards、疗效靶点数据库、人类孟德尔遗传在线、DRUGBANK数据库中查找腰椎间盘突出症的靶点。构建“成分-靶点-疾病”网络,并对蛋白相互作用(protein-protein interaction,PPI)网络进行可视化分析,分析排名前20个靶点的生物过程、分子功能、细胞组成和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)中信号通路。结果筛选出69个桃红四物汤的有效化学成分,235个潜在基因靶点,其中与腰椎间盘突出症共同的基因靶点有82个。PPI网络分析显示,肿瘤坏死因子(tumor necrosis factor,TNF)、表皮生长因子(epidermal growth factor,EGF)、表皮生长因子受体(epidermal growth factor receptor,EGFR)、激活蛋白1(activator protein 1,JUN)、丝氨酸/苏氨酸蛋白激酶(serine/threonine-protein kinase 1,AKT1)是桃红四物汤干预腰椎间盘突出症的核心靶点。基因本体论的富集分析结果显示,桃红四物汤干预腰椎间盘突出症的过程涉及对DNA结合转录因子活性的调节、细胞对生物刺激的反应、氧化应激反应以及对脂多糖、细菌来源分子的调节;KEGG的通路富集分析结果显示,丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)通路、晚期糖基化终末产物(advanced glycation end product,AGE)-AGE受体(receptor for AGE,RAGE)通路、TNF通路的差异蛋白表达水平较高。结论桃红四物汤可能是通过抗炎、提高免疫、调控激素、延缓细胞凋亡等作用多靶点、多通路调控腰椎间盘突出症。
