利用基因工程菌甲醇毕赤酵母(PMAD16/pMETαA-Lcc1)培养产生的重组漆酶,研究了重组漆酶对蒽醌染料Remazol Brilliant Blue R脱色的影响。结果表明:重组漆酶对蒽醌染料RBBR脱色的最佳pH值为4.5,温度为45℃,当溶液中漆酶活力为10.0U/mL时...利用基因工程菌甲醇毕赤酵母(PMAD16/pMETαA-Lcc1)培养产生的重组漆酶,研究了重组漆酶对蒽醌染料Remazol Brilliant Blue R脱色的影响。结果表明:重组漆酶对蒽醌染料RBBR脱色的最佳pH值为4.5,温度为45℃,当溶液中漆酶活力为10.0U/mL时,在最适脱色条件下作用14h,粗漆酶和纯化漆酶对蒽醌染料RBBR(80mg/L)的脱色率分别为95.2%和87.5%,重组漆酶可有效地使蒽醌染料RBBR脱色。展开更多
Objective,Used the TG/DTA curves and XRD patterns to identify five kinds of free anthraquinones.Method,The thermograms,differential thermograms and X-ray powder diffraction patterns were obtained by thermal analyzer a...Objective,Used the TG/DTA curves and XRD patterns to identify five kinds of free anthraquinones.Method,The thermograms,differential thermograms and X-ray powder diffraction patterns were obtained by thermal analyzer and X-ray powder diffraction analyzer.Result,By analyzing the TG/DTA curves and XRD patterns of the free anthraquinones(Rhein,Emodin,Aloe-emodin,Chrysophanol and Physcion)from Rheum,the results showed that they had the similar TG curves,however,due to the substituents attached on the 1.8-dihydric anthraquinones are different,every sample had different weight lose.All the DTA curves of free anthraquinones had two obviously characteristic peaks,but there were obviously differences on curvilinear shapes,peak location and peak values.The X-ray powder diffraction patterns of free anthraquinones have their own characteristic and very obvious.Conclusion: According to the differences in the thermal and XRD properties of five free anthraquinones,the kind of free anthraquinones could be easily identified and they can be used as correspondence free anthraquinones’s characteristic fingerprint patterns.展开更多
文摘利用基因工程菌甲醇毕赤酵母(PMAD16/pMETαA-Lcc1)培养产生的重组漆酶,研究了重组漆酶对蒽醌染料Remazol Brilliant Blue R脱色的影响。结果表明:重组漆酶对蒽醌染料RBBR脱色的最佳pH值为4.5,温度为45℃,当溶液中漆酶活力为10.0U/mL时,在最适脱色条件下作用14h,粗漆酶和纯化漆酶对蒽醌染料RBBR(80mg/L)的脱色率分别为95.2%和87.5%,重组漆酶可有效地使蒽醌染料RBBR脱色。
文摘Objective,Used the TG/DTA curves and XRD patterns to identify five kinds of free anthraquinones.Method,The thermograms,differential thermograms and X-ray powder diffraction patterns were obtained by thermal analyzer and X-ray powder diffraction analyzer.Result,By analyzing the TG/DTA curves and XRD patterns of the free anthraquinones(Rhein,Emodin,Aloe-emodin,Chrysophanol and Physcion)from Rheum,the results showed that they had the similar TG curves,however,due to the substituents attached on the 1.8-dihydric anthraquinones are different,every sample had different weight lose.All the DTA curves of free anthraquinones had two obviously characteristic peaks,but there were obviously differences on curvilinear shapes,peak location and peak values.The X-ray powder diffraction patterns of free anthraquinones have their own characteristic and very obvious.Conclusion: According to the differences in the thermal and XRD properties of five free anthraquinones,the kind of free anthraquinones could be easily identified and they can be used as correspondence free anthraquinones’s characteristic fingerprint patterns.
