On-line monitoring of self-flocculating yeast cell p articles (SFYCPs) that were characterized by their weak strength, irregular morp hology, special size distribution, and so on, was realized by using the Focused Bea...On-line monitoring of self-flocculating yeast cell p articles (SFYCPs) that were characterized by their weak strength, irregular morp hology, special size distribution, and so on, was realized by using the Focused Beam Reflectance Measurement (FBRM) technique.The experimental results showed go od correlation between the total counts of the FBRM chord length and the biomass concentration of SFYCPs, which could be used to measure the biomass concentrati on for the SFYCP system quantitatively.Furthermore, the mode of FBRM chord lengt h distribution was linearly correlated to both the mean (R2=0.986) and th e 95th percentile (R2=0.988)of the length-weighted chord length distribu tion, which could be used to evaluate SFYCP size distribution effectively.The CO 2 bubbles produced during cell growth/fermentation were observed and proved to create disturbance on on-line measurement of the SFYCP system.This disturbance could be excluded by estimating SFYCP size distribution based on the mode of FB RM chord length distribution.展开更多
文摘On-line monitoring of self-flocculating yeast cell p articles (SFYCPs) that were characterized by their weak strength, irregular morp hology, special size distribution, and so on, was realized by using the Focused Beam Reflectance Measurement (FBRM) technique.The experimental results showed go od correlation between the total counts of the FBRM chord length and the biomass concentration of SFYCPs, which could be used to measure the biomass concentrati on for the SFYCP system quantitatively.Furthermore, the mode of FBRM chord lengt h distribution was linearly correlated to both the mean (R2=0.986) and th e 95th percentile (R2=0.988)of the length-weighted chord length distribu tion, which could be used to evaluate SFYCP size distribution effectively.The CO 2 bubbles produced during cell growth/fermentation were observed and proved to create disturbance on on-line measurement of the SFYCP system.This disturbance could be excluded by estimating SFYCP size distribution based on the mode of FB RM chord length distribution.
