The pps A gene encoding PpsA was obtained by polymerase chain reaction(PCR) and expressed in E.coli BL21 strain.SDS PAGE demonstrated that the band at 87 kD was more intensive than the same bands of the constract.The ...The pps A gene encoding PpsA was obtained by polymerase chain reaction(PCR) and expressed in E.coli BL21 strain.SDS PAGE demonstrated that the band at 87 kD was more intensive than the same bands of the constract.The specific activity of PpsA in crude extracts was increased 3.9 fold.展开更多
文摘The pps A gene encoding PpsA was obtained by polymerase chain reaction(PCR) and expressed in E.coli BL21 strain.SDS PAGE demonstrated that the band at 87 kD was more intensive than the same bands of the constract.The specific activity of PpsA in crude extracts was increased 3.9 fold.
